Fish Detection of Campylobacter and Arcobacter Adhered to Stainless Steel Coupons

Šilhová, Lucie; Moťková, Petra; Šilha, David; Vytřasová, Jarmila

doi:10.15414/jmbfs.2015.4.4.347-351

Cited by 2 publications

(2 citation statements)

References 3 publications

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“…FISH was used by Almeida, Azevedo, Santos, Keevil, and Vieira [ 158 ] to characterize and quantify the biofilm formation of S. enterica , L. monocytogenes , and E. coli on different surfaces (e.g., glass, stainless steel) using PNA probes. Stainless steel was also used as a surface to capture biofilm formation of various Arcobacter species using FISH in a study by Šilhová, Moťková, Šilha, and Vytřasová [ 161 ]. Bragança, Azevedo, Simões, Keevil, and Vieira [ 159 ] screened natural biofilms in a drinking water distribution system for the occurrence of H. pylori using PNA-FISH and evaluated the composition of natural biofilms from conveyors in breweries.…”

Section: Specific State-of-the-art Flow-fish Methods and Applications For Monitoring And Detectionmentioning

confidence: 99%

Potential of Flow Cytometric Approaches for Rapid Microbial Detection and Characterization in the Food Industry—A Review

Zand

Froehling

Schoenher

et al. 2021

Foods

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As microbial contamination is persistent within the food and bioindustries and foodborne infections are still a significant cause of death, the detection, monitoring, and characterization of pathogens and spoilage microorganisms are of great importance. However, the current methods do not meet all relevant criteria. They either show (i) inadequate sensitivity, rapidity, and effectiveness; (ii) a high workload and time requirement; or (iii) difficulties in differentiating between viable and non-viable cells. Flow cytometry (FCM) represents an approach to overcome such limitations. Thus, this comprehensive literature review focuses on the potential of FCM and fluorescence in situ hybridization (FISH) for food and bioindustry applications. First, the principles of FCM and FISH and basic staining methods are discussed, and critical areas for microbial contamination, including abiotic and biotic surfaces, water, and air, are characterized. State-of-the-art non-specific FCM and specific FISH approaches are described, and their limitations are highlighted. One such limitation is the use of toxic and mutagenic fluorochromes and probes. Alternative staining and hybridization approaches are presented, along with other strategies to overcome the current challenges. Further research needs are outlined in order to make FCM and FISH even more suitable monitoring and detection tools for food quality and safety and environmental and clinical approaches.

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Section: Specific State-of-the-art Flow-fish Methods and Applications For Monitoring And Detectionmentioning

confidence: 99%

Potential of Flow Cytometric Approaches for Rapid Microbial Detection and Characterization in the Food Industry—A Review

Zand

Froehling

Schoenher

et al. 2021

Foods

View full text Add to dashboard Cite

show abstract

“…Arcobacter spp. tem sido também relatados como já isolados frequentemente de miúdos e carne de frango ,no geral (Fernandes et al, 2015, Villarrruel-Lopes et al, 2003, Fernandes et al, 2010, Karadas et al, 2013.Também já foram reportados em peito de frango (Fallas-Padilha et al, 2014. Oliveira et al, 2018 e em carcaças de frango (Oliveira et al ,2001, Eifert et al, 2003, Ávila et al, 2011 cryaerophilus (Oliveira et al, 2018).…”

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Ocorrência de <i>Arcobacter</i> spp. em carne de frango

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Occurence of Arcobacter spp. in poultry meat Arcobacter spp., previously known as aerotolerant Campylobacter, is considered a bacterial genus that includes species considered emerging pathogens that can be transmitted by food. Arcobacter has been associated with gastroenteritis, persistent diarrhea and bacteremia in humans. It is a gram negative, thermosensitive bacterium, although it can survive at 4 o C. In Brazil, there are few studies of the occurrence of Arcobacter in animal products including those of animal origin, especially those most consumed, such as poultry and pork. There are occasional studies of their occurrence in cooled products, such as cuts and in refrigerated chicken carcasses. The objective of this study was to evaluate the occurrence of Arcobacter spp. in refrigerated chicken cuts and carcasses of the retail and in chicken thighs free of antibiotic and organic refrigerated from slaughterhouse by conventional isolation and genotyping by polymerase chain reaction (PCR). A total of 153 chicken meat samples were analyzed, of which 39.21% (59/153) were positive for the Arcobacter genus. A total of sixty-four isolates positive for Arcobacter spp., corresponding 89.06% (57/64) of A. lacus, 4.7% (3/64) of A. thereius, 3.12% (2/64) of A. butzleri, and 3.12% (2/64) of Arcobacter spp. species not yet identified. Phenotypic resistance analyzes were performed on 12 antibiotics with 34 isolates, previously selected from four different sources of chicken meat obtained in this study, and three strains used as positive control of Arcobacter spp. Phenotypic resistance to antimicrobials were 100% for nalidixic acid and clindamycin , 29.73% for erythromycin, 24.32% for kanamycin, 21.62% for tetracycline, 18.42% for chloramphenicol, 13.51% for gentamycin, 8.11% for streptomycin, 5.41% for azithromycin and ciprofloxacin, 2.10% for vancomycin and 0.00% for ampicillin.

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Fish Detection of Campylobacter and Arcobacter Adhered to Stainless Steel Coupons

Cited by 2 publications

References 3 publications

Potential of Flow Cytometric Approaches for Rapid Microbial Detection and Characterization in the Food Industry—A Review

Potential of Flow Cytometric Approaches for Rapid Microbial Detection and Characterization in the Food Industry—A Review

Ocorrência de <i>Arcobacter</i> spp. em carne de frango

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