Fish bone versus fish demineralized bone matrix (vertebra) effects on healing of experimental radial defect in rat model

Abstract

Surgeons conducting bone grafting procedures have been in search of a bone graft substitute to avoid autogenous bone harvesting because of its associated complications. In the present study, the authors used fish bone and fish DBM in healing of a radial bone defect in a rat model. The 16 female Sprague-Dawley rats used in the present study were divided into the following four groups: autograft (positive control), untreated defect (negative control), and fish DBM, and fish bone graft as the experimental groups. Radiographs were taken in the 1st, 35th, and 56th post-operative days. Histopathological and biomechanical evaluations were performed in 56th post-operative day. The radiological, histopathological, and biomechanical evaluations revealed a superior rate of bone formation in the fish bone graft and autograft groups compared to those of the control. This study showed that the fish bone graft is effective in healing of the radial bone defect in rat.

Toxicity of Natural Hydroxyapatite

“…Oryan et al [19] evaluated salmon fish bone and demineralized bone matrix implanted in the radial bone defect model (murine study). Radiographic, histopathological and biochemical evaluation was found to be favorable.…”

From bone to nanoparticles: development of a novel generation of bone derived nanoparticles for image guided orthopedic regeneration

“…[1][2][3][4][5] Although bone is a living tissue that is capable of continuous regeneration, disturbances in bone homeostasis can lead to impaired bone healing. 6,7 Pharmacologic therapy is the primary clinical treatment for such bone diseases; 8 however, unlike soft organs such as the liver or kidney, bone is highly mineralized and less vascularized, which ultimately decreases the efficiency of drug delivery to bone. 9,10 Another limitation of current pharmacologic therapies is that many drugs have a short circulation half-life, resulting in poor assimilation and a low bioavailability in the diseased bone.…”

“…13,14 Bone is a heterogeneous composite tissue, composed of a dynamic extracellular matrix (ECM) network that consists of 50-70% inorganic minerals, 20-40% organic matrix, and 5-10% water. 6,7 The inorganic minerals, primarily consisting of calcium phosphate (CaP), specifically hydroxyapatite (HA), are the source for hardness and strength of the bone, while the organic matrix, including collagens and non-collagenous proteins, provide the tissue with flexibility and a template to regulate new bone formation. 15,16 Collagens, especially collagen type I, are the most abundant proteins found in bone and play a critical role in their structure, tissue organization, and mechanical support.…”