First trimester plasma microRNAs levels predict Matsuda Index-estimated insulin sensitivity between 24th and 29th week of pregnancy

Abstract

IntroductionGestational diabetes mellitus (GDM) is a consequence of an imbalance between insulin sensitivity (IS) and secretion during pregnancy. MicroRNAs (miRNAs) are small and secreted RNA molecules stable in blood and known to regulate physiological processes including glucose homeostasis. The aim of this study was to identify plasmatic miRNAs detectable in early pregnancy predicting IS at 24th-29th week of pregnancy.Research design and methodsWe quantified circulating miRNAs in 421 women in plasma collected at 9.6±2.2 weeks of pregnancy using next-generation sequencing.Resultswe detected 2170 miRNAs: 39 (35 positively and 4 negatively) were associated with IS as estimated by the Matsuda Index at 26.4±1.0 weeks of pregnancy. Lasso regression identified 18 miRNAs independently predicting Matsuda Index-estimated IS. Together with gestational age, maternal age and body mass index at first trimester, they explain 36% of IS variance in late second trimester of pregnancy. These miRNAs regulate fatty acid biosynthesis and metabolism among other pathways.ConclusionsIn summary, we have identified first trimester plasmatic miRNAs predictive of Matsuda Index-estimated IS in late second trimester of pregnancy. These miRNAs could also contribute to initiate and support IS adaptation to pregnancy potentially through lipid metabolism regulation.

Analysis of Circulating C19MC MicroRNA as an Early Marker of Hypertension and Preeclampsia in Pregnant Patients: A Systematic Review

“…Regarding year of publication, 3.33% of the articles were published in 2013 [ 1 ], 2014 [ 19 ], or 2015 [ 13 ], and 6.67% of the reports were published in 2016 [ 22 , 23 ] and 2019 [ 2 , 12 ]. In addition, 10% of the studies were published in 2017 [ 4 , 24 , 25 ] and 2018 [ 15 , 21 , 26 ], while 13.33% of the articles were published in 2021 [ 3 , 8 , 14 , 17 ] and 2022 [ 6 , 10 , 27 , 28 ]. Finally, 30% of the studies were published in 2020 [ 5 , 7 , 9 , 11 , 16 , 18 , 20 , 29 , 30 ].…”

“…Consequently, subsequent large-scale investigations are required to evaluate the positive predictive value, specificity, and sensitivity of C19MC microRNAs for hypertension or pre-eclampsia. The effectiveness of placental-specific microRNAs for diagnosing disease severity should be assessed in connection with Doppler ultrasonography characteristics, delivery needs, and clinical symptoms [ 10 ]. The research produced intriguing results, showing that up-regulation of circulating C19MC microRNAs is a hallmark of early pregnancy, which is predisposed to developing issues linked to gestational hypertension and placental insufficiency.…”

“…Although combinations of second-trimester biochemical indicators, markers, and ultrasonography have been proposed, none has yet shown findings that are sound enough to be used in a therapeutic setting [ 19 ]. In the first trimester of maternal serum/plasma samples collected from patients with hypertension or pre-eclampsia, a number of the hypothesized targets of C19MC microRNAs in which the current study was interested were previously shown to be enhanced [ 10 ]. Several miRNAs may control the same gene.…”

Maternal Body Mass Index Is Associated with Profile Variation in Circulating MicroRNAs at First Trimester of Pregnancy

“…In another previous study from our group and using the same Gen3G microtranscriptomic dataset, we identified miRNAs in the first trimester of pregnancy associated with and predictive of insulin sensitivity in the second trimester of pregnancy. Among our replicated miRNAs, 15 were also associated with insulin sensitivity [ 17 ]. Similar analyses were also conducted to identify miRNAs associated and predictive of GDM still using this microtranscriptomics and Gen3G datasets.…”

“…Indeed, miRNAs are suspected of having an important role in pregnancy, its maintenance as well as adaptation to its very specific physiologic needs [ 10 , 11 ]. Accordingly, dysregulation of the C19MC and C14MC has been associated with pregnancy complications, including PE, intrauterine growth restriction and insulin sensitivity regulation [ 13 , 14 , 15 , 16 , 17 ].…”