First study of pathogen load and localisation of ovine footrot using fluorescence in situ hybridisation (FISH)

Witcomb, Luci A.; Green, Laura; Calvo-Bado, Leonides; Russell, Claire; Smith, Edward M.; Grogono-Thomas, Rosemary; Wellington, Elizabeth M. H.

doi:10.1016/j.vetmic.2015.01.022

Cited by 24 publications

(30 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Studies using whole biopsies reported eubacteria presence in healthy ovine interdigital skin (Calvo-Bado et al 2011;Maboni et al 2016;Witcomb et al 2015). Interestingly, we detected eubacterial DNA down to depths of 3mm in healthy samples.…”

Section: Discussionmentioning

confidence: 53%

“…Previous studies based on Giemsa stain and fluorescent in situ hybridisation (FISH) have suggested D. nodosus was primarily localised in superficial epidermis of ovine interdigital skin (Egerton et al 1969;Witcomb et al 2015). However, Witcomb and colleagues reported a single cell of D.…”

Section: Discussionmentioning

confidence: 99%

“…nodosus in the dermis of footrot infected ovine skin (Witcomb et al 2015). In contrast to FISH, qPCR technique is able to amplify target DNA, thereby detecting low levels of D. nodosus.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Peer Review #2 of "Novel inflammatory cell infiltration scoring system to investigate healthy and footrot affected ovine interdigital skin (v0.1)"

2018

View full text Add to dashboard Cite

Ovine footrot is a degenerative disease of sheep feet leading to the separation of hoofhorn from the underlying skin and lameness. This study quantitatively examined histological features of the ovine interdigital skin as well as their relationship with proinflammatory cytokine (IL-1β) and virulent Dichelobacter nodosus in footrot. From 55 healthy and 30 footrot ovine feet, parallel biopsies (one fixed for histology) were collected post-slaughter and analysed for lesions and histopathological analysis using haematoxylin and eosin and Periodic Acid-Schiff. Histological lesions were similar in both conditions while inflammatory scores mirror IL-1β expression levels. Increased inflammatory score corresponded with high virulent D. nodosus load and was significant (p<0.0001) in footrot feet with an inflammatory score of 3 compared to scores 1 and 2. In addition, in contrast to healthy tissues, localisation of eubacterial load extended beyond follicular depths in footrot samples. The novel inflammatory cell infiltration scoring system in this study may be used to grade inflammatory response in the ovine feet and demonstrated an association between severity of inflammatory response and increased virulent D. nodosus load.

show abstract

Section: Discussionmentioning

confidence: 53%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Peer Review #2 of "Novel inflammatory cell infiltration scoring system to investigate healthy and footrot affected ovine interdigital skin (v0.1)"

2018

View full text Add to dashboard Cite

show abstract

“…Variability of reduction within disinfectant groups was observed and is explainable by various factors influencing the ex vivo experiment. D. nodosus loads vary naturally among feet due to individual differences and the different clinical status of footrot affected feet, leading to different prevalues [28]. Furthermore, the total number of microorganisms can also affect the activity of a disinfectant.…”

Section: Discussionmentioning

confidence: 99%

In vitro and ex vivo testing of alternative disinfectants to currently used more harmful substances in footbaths against Dichelobacter nodosus

Hidber

Pauli²,

Steiner

et al. 2020

PLoS ONE

View full text Add to dashboard Cite

A footbath-based control program for ovine footrot, a contagious disease caused by Dichelobacter nodosus, will be implemented in Switzerland. The currently used footbath disinfectants formaldehyde, zinc sulfate and copper sulfate are carcinogenic or environmental pollutants. Hence, the aim of this study was to identify alternative disinfectants, which are highly effective, non-carcinogenic, environmentally acceptable, inexpensive, available as concentrate and suitable for licensing. The antimicrobial effect of a series of potential chemicals such as lactic acid, propionic acid, hydrogen peroxide, sodium hypochlorite, octenidine dihydrochloride, chlorocresol, Ampholyt 20 and the registered biocide DESINTEC® Hoof Care Special D (Desintec) were investigated by culture based in vitro testing. The microcidal effect of various Desintec concentrations were then compared against routinely used 4% formaldehyde and 10% zinc sulfate in ex vivo assays on sheep feet from slaughter. For this purpose a newly established PMA (propidium monoazid) real-time PCR using the improved dye PMAxx™ was applied that allows discrimination of viable and dead D. nodosus. In the ex vivo experiments, 4% formaldehyde was significantly more effective than 10% zinc sulfate and was chosen as positive control for assessing the new disinfectant. The disinfectant effect of Desintec in a minimal concentration of 6% was equally effective as 4% formaldehyde, meaning that it offers a comparable antimicrobial effect against virulent D. nodosus. In conclusion, Desintec is a promising disinfectant for replacing formaldehyde, copper sulfate and zinc sulfate in footbaths against footrot.

show abstract

“…Until recently the pathogenesis of footrot was thought to be due to an initial infection by F. necrophorum followed by a secondary infection with D. nodosus (11), but recent studies suggest otherwise. Regardless, both bacteria play an important role in footrot infection, with D. nodosus possibly driving the pathogenesis of footrot from the initiation of interdigital dermatitis through to the development of severe footrot, and F. necrophorum likely attributing to the severity and duration of the infection (12,13). In cases of foot abscesses, F. necrophorum has been associated in conjunction with T. pyogenes (8,14).…”

Section: Introductionmentioning

confidence: 99%

Infectious lameness among migratory sheep and goats in north-west India, with particular focus on anaerobes

Wani¹,

Verma²,

Sharma³

et al. 2015

Rev. Sci. Tech. OIE

View full text Add to dashboard Cite

Various bacterial species, particularly Dichelobacter nodosus, Fusobacterium necrophorum, Trueperella pyogenes (previously Arcanobacterium pyogenes) and Treponema spp., have been implicated in infectious conditions manifesting in lameness in sheep and goat populations. The current study reports the causes of infectious lameness in the north-western Himalayan region, particularly Himachal Pradesh (HP), where no such study has been conducted in the past. Among a total surveyed population of 27,586 animals, comprised of 15,006 sheep and 12,580 goats, 216 samples were collected from foot lesions. A total of 6.48% (14/216) samples were positive for D. nodosus, 20.83% (45/216) for F. necrophorum and 20.37% (44/216) for T. pyogenes. In three instances, all of the three aforementioned bacteria were present in a single foot lesion. Most of the positive samples were from areas in the state of HP adjoining the state of Jammu and Kashmir (J&K), where footrot is endemic. The confirmation of footrot in HP and the fact that F. necrophorum and T. pyogenes were detected from cases of foot/hoof infection in high numbers emphasises that these organisms play an important role in inducing morbidity in migratory sheep and goats. The present investigation also confirms the detection of D. nodosus from cases of footrot for the first time from HP. With new reports identifying F. necrophorum as a pathogen with a potential role in aggravating infection caused by footrot, the development of a combined vaccine to prevent lameness in sheep and goats in the north-western Himalayan region has been suggested.

show abstract

First study of pathogen load and localisation of ovine footrot using fluorescence in situ hybridisation (FISH)

Cited by 24 publications

References 32 publications

Peer Review #2 of "Novel inflammatory cell infiltration scoring system to investigate healthy and footrot affected ovine interdigital skin (v0.1)"

Peer Review #2 of "Novel inflammatory cell infiltration scoring system to investigate healthy and footrot affected ovine interdigital skin (v0.1)"

In vitro and ex vivo testing of alternative disinfectants to currently used more harmful substances in footbaths against Dichelobacter nodosus

Infectious lameness among migratory sheep and goats in north-west India, with particular focus on anaerobes

Contact Info

Product

Resources

About