First Steps towards a near Real-Time Modelling System of Vibrio vulnificus in the Baltic Sea

Schütt, Eike M.; Hundsdörfer, Marie; Hoyningen-Huene, Avril Jean Elisabeth von; Lange, Xaver; Koschmider, Agnes; Oppelt, Natascha

doi:10.3390/ijerph20085543

Cited by 3 publications

(1 citation statement)

References 74 publications

(144 reference statements)

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“…distribution is an accurate identification of Vibrio species that most frequently cause infections in humans, but especially of the most harmful V. vulnificus . Pinpointing a timeframe of increased risk of Vibrio infection is the next step in curbing the increasing numbers of infections and also has wide-ranging ecological and economic effects [ 2 , 19 ]. In the context of the Baltic Sea, where diverse Vibrio species coexist, precise as well as simple and cost-effective identification becomes more critical.…”

Section: Introductionmentioning

confidence: 99%

Combined TCBS and CHROMagar Analyses Allow for Basic Identification of Vibrio vulnificus within a 48 h Incubation Period in the Coastal Baltic Sea

Glackin,

Dupke,

Chandra

et al. 2024

Microorganisms

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With rising infection rates in recent years, Vibrio vulnificus poses an increasing threat to public safety in the coastal brackish Baltic Sea. It is therefore important to monitor this organism and assess the V. vulnificus infection risk on a more regular basis. However, as the coastline of the Baltic Sea is 8000 km long and shared by nine nations, a convenient, fast, inexpensive, yet efficient V. vulnificus identification method is essential. We evaluated the effectiveness of a two-step agar-based approach consisting of successive Vibrio isolation and cultivation on thiosulphate-citrate-bile salt sucrose (TCBS) agar and CHROMagar™ Vibrio for V. vulnificus in comparison with V. cholerae, V. parahaemolyticus, and V. alginolyticus. Our study contains isolates from water and sediment across a broad expanse of the Baltic Sea including 13 locations and two different summers, the time of year during which Vibrio infections are usually much more frequent. Confirmation of isolate species identity was carried out using molecular analyses. The two-step agar plating method performed well across different locations and timeframes in correctly identifying V. vulnificus by more than 80%, but the sensitivity in other Vibrio species varied. Thus, our approach yielded promising results as a potential tool for early V. vulnificus detection across a broad timeframe and transect of the Baltic Sea and potentially other brackish environments.

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Section: Introductionmentioning

confidence: 99%

Combined TCBS and CHROMagar Analyses Allow for Basic Identification of Vibrio vulnificus within a 48 h Incubation Period in the Coastal Baltic Sea

Glackin,

Dupke,

Chandra

et al. 2024

Microorganisms

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Bakterielle Infektionen der Haut im Kontext von Klimawandel und Migration

Klapproth,

Ruegamer

et al. 2023

Dermatologie

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The ecology of potentially pathogenicVibriospp. in a seagrass meadow ecosystem

Gebbe,

Kesy,

Hallier

et al. 2024

Preprint

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Seagrass meadow ecosystems offer several valuable ecosystem services in coastal regions around the world. Recent studies have suggested that one such important service is reduction of pathogenic bacteria and specificallyVibriospp. in adjacent waters. The specific mechanisms of pathogen reduction remain unclear, although increased sedimentation has been suggested as one likely process for pathogens to be quenched from the water column. WhetherVibriospp. persist in the sediment or in other compartments of the seagrass meadow is currently not known, but it has been shown that marine surface biofilms can function as reservoirs of pathogenic vibrios. This general feature may also apply to seagrass and sediment surfaces. In this study, we investigated the relative abundance and community ecology ofVibriospp. bacteria in Baltic Sea seagrass meadows using both culturing and culture-independent methods. While we did not detect a significant reduction ofVibriospp., the highest relative abundances ofVibriospp. were observed in the water column above unvegetated sites as compared to seagrass meadows. We also detected high relative abundances ofVibriospp. on seagrass roots, supporting previous observations that marine surfaces are selectively colonized byVibriospp., implying that these habitats are important for the persistence and possibly release ofVibriospp. into the water column. Our results emphasize the need to understand the interactions of pathogenic bacteria with coastal habitats, including interactions with host organisms such as seagrasses that provide biofilm microenvironments, in order to understand how diseases associated with these organisms develop.

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First Steps towards a near Real-Time Modelling System of Vibrio vulnificus in the Baltic Sea

Cited by 3 publications

References 74 publications

Combined TCBS and CHROMagar Analyses Allow for Basic Identification of Vibrio vulnificus within a 48 h Incubation Period in the Coastal Baltic Sea

Combined TCBS and CHROMagar Analyses Allow for Basic Identification of Vibrio vulnificus within a 48 h Incubation Period in the Coastal Baltic Sea

Bakterielle Infektionen der Haut im Kontext von Klimawandel und Migration

The ecology of potentially pathogenicVibriospp. in a seagrass meadow ecosystem

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