First Steps in the Development of a Functional Assay for Human Sperm Using Pig Oocytes

Cánovas, Sebastián; Coy, Pilar; Gómez, E.

doi:10.2164/jandrol.106.001081

Cited by 9 publications

(6 citation statements)

References 61 publications

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“…So, ICSI is presented as one alternative way for in vitro production of monospermic zygotes. Moreover, recent findings show that heterologous ICSI could represent a powerful tool to study human sperm functionality using the pig as oocyte donor (Canovas et al. 2007).…”

Section: Origins and Applications Of Icsimentioning

confidence: 99%

Intracytoplasmic Sperm Injection in Livestock Species: An Update

García-Roselló

García-Mengual

Coy

et al. 2009

Reprod Domestic Animals

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Intracytoplasmic sperm injection (ICSI) is a powerful technique in the field of assisted reproduction (ART) and provides exciting opportunities for studying the basic mechanisms of fertilization and early embryo development. Nevertheless, its application in agriculture and conservation biology has been greatly hampered by the low success rate reported for this method in respect of economically important species. Specifically, the rates of blastocyst formation and live newborn are greatly reduced when zygotes are generated by ICSI. Except for humans, ICSI remains a low efficiency technology in comparison with alternatives such as in vitro fertilization (IVF) and its application is less widespread. In this paper, we discuss the present status, applications and factors affecting ICSI in pigs and other species.

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Section: Origins and Applications Of Icsimentioning

confidence: 99%

Intracytoplasmic Sperm Injection in Livestock Species: An Update

García-Roselló

García-Mengual

Coy

et al. 2009

Reprod Domestic Animals

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“…These authors suggested that this difference correlates with the difference in the structure and composition of mouse and human or rat ZP. In addition, human spermatozoa have the capacity to bind to porcine ZP [47] but not to mouse ZP [17]. Many equine spermatozoa are able to bind to bovine ZP [48], but few bound to porcine ZP in our experiment.…”

Section: Discussionmentioning

confidence: 53%

“…Previous studies showed that bovine cytoplasm was able to decondense equine and human sperm chromatin after ICSI [66,67] and that porcine cytoplasm was able to decondense ovine, mouse, and human sperm chromatin after ICSI [47,68]. These data suggest that the molecules involved in sperm decondensation are at least partially conserved between mammalian species.…”

Section: Discussionmentioning

confidence: 62%

New Insights into the Mechanisms of Fertilization: Comparison of the Fertilization Steps, Composition, and Structure of the Zona Pellucida Between Horses and Pigs1

Mugnier

Dell’Aquila

Peláez

et al. 2009

Biology of Reproduction

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The mechanism of fertilization remains largely enigmatic in mammals. Most studies exploring the molecular mechanism underlying fertilization have been restricted to a single species, generally the mouse, without a comparative approach. However, the identification of divergences between species could allow us to highlight key components in the mechanism of fertilization. In the pig, in vitro fertilization (IVF) and polyspermy rates are high, and spermatozoa penetrate easily through the zona pellucida (ZP). In contrast, IVF rates are low in the horse, and polyspermy is scarce. Our objective was to develop a comparative strategy between these two divergent models. First, we compared the role of equine and porcine gametes in the following five functions using intraspecific and interspecific IVF: ZP binding, acrosome reaction, penetration through the ZP, gamete fusion, and pronucleus formation. Under in vitro conditions, we showed that the ZP is a determining element in sperm-ZP attachment and penetration, whereas the capacity of the spermatozoa is of less importance. In contrast, the capacity of the spermatozoa is a key component of the acrosome reaction step. Second, we compared the composition and structure of the equine and porcine ZP. We observed differences in the number and localization of the ZP glycoproteins and in the mesh-like structure of the ZP between equine and porcine species. These differences might correlate with the differences in spermatozoal attachment and penetration rates. In conclusion, our comparative approach allows us to identify determining elements in the mechanism of fertilization.

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“…Sperm to zona attachment experiments were conducted as described previously [29] with some modifications. The number of attached spermatozoa was evaluated in oocytes from the homologous and heterologous IVF groups at 2.5 h of co-incubation 155 time.…”

Section: Assessment Of Attachment and Ivf Parametersmentioning

confidence: 99%

Heterologous murine and bovine IVF using bottlenose dolphin (Tursiops truncatus) spermatozoa

et al. 2015

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20Assisted reproductive technologies (ARTs) are of great importance for increasing the genetic diversity in captive animals. The use of bovine or murine oocytes in heterologous IVF provide advantages compared to homologous IVF in non-domestic animals, such as the accessibility to oocytes and the availability of well-developed in vitro maturation systems. The aim of this study was to determine the heterologous IVF 25 parameters using cryopreserved dolphin spermatozoa and zona-intact bovine or murine oocytes, as well as to examine the nuclear chromatin status of the dolphin spermatozoa.All the processes involved in the fertilization including embryo cleavage were observed by confocal microscopy and hybrid embryo formation was confirmed by PCR.Heterologous bovine IVF showed no polyspermy, lower percentages of pronuclear 30 formation and a lower cleavage rate compared to homologous IVF group (34.8 vs.89.3%). Heterologous murine IVF showed a lower cleavage rate than homologous IVF (9.6 vs. 77.1%). With respect to dolphin sperm chromatin, it was more stable, i.e. more resistant to EDTA-SDS decondensation than the bovine sperm chromatin. This study revealed the stability of the dolphin sperm chromatin and the ability of the dolphin 35 spermatozoa to penetrate zona-intact bovine and murine oocytes, leading to hybrid embryo formation.

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First Steps in the Development of a Functional Assay for Human Sperm Using Pig Oocytes

Cited by 9 publications

References 61 publications

Intracytoplasmic Sperm Injection in Livestock Species: An Update

Intracytoplasmic Sperm Injection in Livestock Species: An Update

New Insights into the Mechanisms of Fertilization: Comparison of the Fertilization Steps, Composition, and Structure of the Zona Pellucida Between Horses and Pigs1

Heterologous murine and bovine IVF using bottlenose dolphin (Tursiops truncatus) spermatozoa

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