First seroprevalence and molecular identification report of Brucella canis among dogs in Greater Cairo region and Damietta Governorate of Egypt

Hamdy, Mahmoud; Abdel-Haleem, Mahmoud H.; Dawod, Rehab E.; Ismail, Rania I.; Hazem, Soliman S.; Abdel‐Hamid, Nour H.

doi:10.14202/vetworld.2023.229-238

Cited by 5 publications

(3 citation statements)

References 35 publications

(68 reference statements)

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“…Dogs in Colombia had a seroprevalence of 1.96% for B. canis [31]. In Egypt, research showed an apparent prevalence of 3.8% and an actual prevalence of 13.2%, with stray dogs having a higher estimated true prevalence of 15% compared to owned dogs at 12.5% [32]. Positive B. canis antibodies were also found in studies conducted in Italy, where 25 out of 2328 sera were positive (1.1%), and Brazil, where 72 out of 280 sera (25.7%) were positive results.…”

Section: Molecular Identificationmentioning

confidence: 99%

Detection of Brucella canis infection in Pit Bull breed dogs in Turkey

Özavci,

Yüksel Dolgun,

Seferoğlu

et al. 2023

RC FCV-LUZ

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Brucella canis infection is an often neglected but important zoonotic disease. This study aims to determine its seroprevalence in Pit Bull dogs from the Western Region of the Turkish Anatolian Peninsula. In the Province of Manisa, 2 mL blood samples were taken from the antebrachial region of 35 Pit Bull dogs using sterile K2EDTA (3.6 mg) blood tubes, and the samples were analyzed using both the mercaptoethanol (ME) microagglutination test and B. canis–specific PCR techniques. Of the 35 dogs tested by 2–ME RSAT, 13 (37.14%) tested positive and 22 (63%) tested negative. Of the 13 dogs that tested positive for 2–ME RSAT, 8 (22.85%) were female, and 5 (14.28%) were male. Subsequent PCR analysis of all samples revealed that 7 (20%; 7/35) of the samples that tested positive for 2–ME RSAT were actually B. canis–specific PCR positive. These findings suggest that B. canis is present in Pit Bull dogs, although they provide a general idea of the disease's prevalence of the disease in the region. Multicentre studies with larger numbers of cases in different groups of Pit Bulls, such as healthy, patient and risk groups, are needed to provide comprehensive evidence.

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Section: Molecular Identificationmentioning

confidence: 99%

Detection of Brucella canis infection in Pit Bull breed dogs in Turkey

Özavci,

Yüksel Dolgun,

Seferoğlu

et al. 2023

RC FCV-LUZ

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“…Twenty EDTA blood samples were collected aseptically from the jugular veins of positive camels in Oxoid signal blood culture bottles and sent to the lab in an ice box. 22 Culture systems were incubated anaerobically at 37 °C, and the cultures that showed growth within 3 days were incubated both aerobically and anaerobically and examined daily for a week by allowing the blood broth mixture to flow over. The loop of growth is streaked on Tryptic soy agar and Brucella agar and incubated for 3-14 days.…”

Section: Isolation Of Bacteriamentioning

confidence: 99%

Identification of Brucella melitensis from camel’s blood by vitek2 and real time polymerase chain reaction

Manivannan,

Ramasamy,

Sundaresan

et al. 2024

Eur J Clin Exp Med

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Introduction and aim. Brucellosis is a zoonotic disease. Experimental clinical and laboratory diagnosis is still facing problems in identifying the organism. The present study will diagnose a Brucella infection in camel blood in Qatar using serological assays. Isolation and identification were performed on a camel blood sample. Brucella in bacterial isolates was determined by real-time polymerase chain reaction (RT-PCR) as a gold standard test. Material and methods. A total of 220 samples, 200 random serum samples, and 20 EDTA blood samples were selected among the above-mentioned random samples, and 20 serum samples from camel handlers were collected from Al Shahaniya prov ince, Qatar. The Rose Bengal test (RBT), buffered antigen plate agglutination test (BAPAT), and enzyme linked immunosorbent assay (cELISA) for the monoclonal antibody in serum samples were performed using commercially available kits. For the molecular detection of Brucella, conventional PCR and real-time PCR (GPS kit) were used for the genus-specific insertion sequence IS711. Brucella melitensis (MICROBOSS Hightech GmbH kit) was used to identify subspecies. Results. The results identified by vitek2 compact (30%) showed B. melitensis in 6 samples out of 20 isolates. Both conventional (66.67%) and RT-PCR (83.33%) analyses supported this, demonstrating the presence of Brucella. These tests also showed that Brucella species were present in Rose Bengal 182/200 (91%), BAPAT 182/200 (91%), and cELISA (90%) 180/200 in camel serum. Conclusion. To conclude, the prevalence of brucellosis in dromedary camels is higher in this region, and as a matter of urgency, measures should be taken to control the disease.

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“…In Egypt, B. melitensis is the most prevalent Brucella species in ruminants [3] followed by B. abortus and less frequently by B. suis biovar 1 and 2 [4,5]. Recently, B. canis DNA was detected in the blood of stray and owned dogs in Egypt [6].…”

Section: Introductionmentioning

confidence: 99%

Brucellosis Seroprevalence, REP-PCR-Based Genotyping, and Virulence-Associated Genes Distribution Among Brucella melitensis Strains Isolated from Ruminants in Kafr Elsheikh Governorate of Egypt

Abdel-Hamid,

Al-Saftawy,

Moawad

et al. 2024

Egyptian Journal of Veterinary Sciences

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rucellosis is a neglected bacterial zoonosis that has a significant impact on public health and the livestock industry. The purpose of this study was to determine the brucellosis seroprevalence, the circulating Brucella species, Brucella virulence-associated genes distribution, and repetitive extragenic palindromic PCR (REP-PCR) genotyping of Brucella isolates among ruminants in the Kafr Elsheikh Governorate. Blood samples were randomly collected from 926 cattle, 422 buffaloes, 301 sheep, and 333 goats in Kafr Elsheikh from January 2021 to December 2022. We determined apparent and true brucellosis prevalence in ruminants in various Kafr Elsheikh locations. Isolation and molecular confirmation (AMOS-PCR), phylogeny based on REP-PCR Brucella genotyping was inferred. Virutyping was performed on B. melitensis differences in virulence patterns. Brucellosis's true prevalence was calculated to be 13.3%, 11.2%, 15.8%, and 16.8% in cattle, buffaloes, sheep, and goats. Brucella isolates (n=8) were recovered from the supramammary lymph nodes and confirmed molecularly (AMOS-PCR) and bacteriologically as B. melitensis biovar 3. The B. melitensis isolates showed three virulence patterns (V1-V3), with V1 being the most common pattern (62.5%). REP-PCR clearly discriminated between the detected B. melitensis bv3 isolates into two clades (C1 and C2) and 7 REP genotypes (G1 -G7). In conclusion, the B. melitensis bv3 high diversity genotypes based on REP-PCR patterns highlight the cost-effective discrimination efficiency of the reproducible REP-PCR. B. melitensis's consistent isolation at low rates across the year of sampling may suggest various previous outbreaks of established infection rather than a recent introduction of foreign strains in the Governorate.

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First seroprevalence and molecular identification report of Brucella canis among dogs in Greater Cairo region and Damietta Governorate of Egypt

Cited by 5 publications

References 35 publications

Detection of Brucella canis infection in Pit Bull breed dogs in Turkey

Detection of Brucella canis infection in Pit Bull breed dogs in Turkey

Identification of Brucella melitensis from camel’s blood by vitek2 and real time polymerase chain reaction

Brucellosis Seroprevalence, REP-PCR-Based Genotyping, and Virulence-Associated Genes Distribution Among Brucella melitensis Strains Isolated from Ruminants in Kafr Elsheikh Governorate of Egypt

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