First RFLP linkage map of red clover (Trifolium pratense L.) based on cDNA probes and its transferability to other red clover germplasm

Isobe, Sachiko; Клименко, Ирина; Ivashuta, Sergey; Gau, Mitsuru; Kozlov, N. N.

doi:10.1007/s00122-003-1412-z

Cited by 42 publications

(39 citation statements)

References 39 publications

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“…Due to the conserved genomic regions between different genome, SSR marker could be transferable among congeneric species (Boutin et al 1995;Isobe et al 2003;Kuleung et al 2004). High transferability of EST-SSRs (34%) and genomic SSR (25%) between soybean and cultivated peanut were reported (He et al 2006;Hong et al 2010).…”

Section: Pcr Amplification and Polymorphism Analysismentioning

confidence: 99%

Identification of SSR markers using soybean (Glycine max) ESTs from globular stage embryos

Li¹,

Zhao²,

Wang³

et al. 2010

Electron. J. Biotechnol.

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Microsatellites, or simple sequence repeats (SSRs), in expressed sequence tags (ESTs) provide an opportunity for low cost SSR development. We looked for EST-SSRs in 403,511 ESTs (generated by 454 sequencing and representing 70,654 contigs and 52,082 singletons) from soybean globular stage embryos. Among 122,736 unique ESTs, 3,729 contained one or more SSRs. In total, 3,989 SSRs were identified including 304 mono, 1,374 di, 2,208 tri, 70 tetra, 13 penta and 20 hexanucleotide SSRs. Thirty three EST-SSRs were selected for primer design and polymorphism analysis using twenty soybean cultivars and one wild-type soybean. Successful amplification was obtained using 21 of these primer pairs, 11 of which detected polymorphisms in these soybean cultivars. These results demonstrated that 454 high throughput sequencing is a powerful tool for molecular marker development. From the 3,989 identified SSRs we expect to obtain a large number of makers with polymorphism among different soybean cultivars, which would be useful for analysis of genetic diversity and maker assisted selection in the soybean breeding programs.

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Section: Pcr Amplification and Polymorphism Analysismentioning

confidence: 99%

Identification of SSR markers using soybean (Glycine max) ESTs from globular stage embryos

Li¹,

Zhao²,

Wang³

et al. 2010

Electron. J. Biotechnol.

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“…The first genetic linkage map with 256 restriction fragment length polymorphism (RFLP) markers was constructed for red clover by Isobe et al (2003) using a mapping population of 188 F 1 individuals derived from a double pseudo-testcross between HR as the female parent and R130 as the male parent. HR was an elite plant selected from early progenies of the cross between Hokuseki and a Swiss variety Renova with characteristics of early flowering, erect shape, red flower colour, and middle-sized leaves.…”

Section: Dna Marker Systems and Genetic Mapsmentioning

confidence: 99%

“…Such a population of red clover useful for segregation analysis, interval mapping, and QTL investigation was constructed by Isobe et al (2003). A mappingbased approach was used to identify SSRs closely linked to the GSI-locus (Riday & Krohn 2010).…”

Section: Candidate Gene Identificationmentioning

confidence: 99%

Modern methods for genetic improvement of Trifolium pratense.

Řepková¹,

Nedĕlník²

2014

CZECH J GENET PLANT

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This review focuses on trends in genetic improvement of a significant representative forage crop, Trifolium pratense (red clover) classified taxonomically into the agronomically outstanding family Fabaceae. Red clover breeding is aimed at improving traits like persistency, resistance to biotic and abiotic factors, forage yield and quality characteristics such as protein quality and stability. Isoflavone content in forage is important for cattle reproduction. Interspecific hybridization of red clover with the related wild species T. medium was used for the introgression of useful traits into red clover. The breeding strategy for the new variety Pramedi included hybrid plants with different DNA contents, repeated backcrosses with red clover, followed by family selection. New techniques of molecular genetics are becoming available to breeders for transferring key and complex traits into improved red clover varieties. Techniques such as marker-assisted selection and candidate gene identification can increase the speed and precision with which traits may be selected. Comparative sequence data analyses helped to identify genes for polyphenol oxidase enzymes and isoflavone synthase in red clover useful for DNA genotyping of individuals and breeding of improved high-quality red clover varieties.Keywords: candidate gene; genetic marker; molecular genetics; red clover; wide hybridization Throughout the history of plant breeding, breeders have endeavoured to improve crop yields and quality, always in the context of the latest knowledge and demands of users. This paper focuses on trends in genetic improvement of a significant representative forage crop, Trifolium pratense L. (red clover), by modern methods. The review summarizes current knowledge of the red clover genome and prospects for using molecular genetics to improve that genome.The genus Trifolium L. is classified taxonomically into the tribe Trifoliae of the large and agronomically outstanding family Fabaceae. It comprises approximately 240 species (Zohary & Heller 1984) of annual and perennial herbs commonly known as clovers, both wild and cultivated. Red clover is an important forage legume that is widely cultivated in most temperate regions and is grown in pastures for grazing, sown as a companion crop, and harvested for hay (Smith et al. 1985; Taylor & QueenSberry 1996). It is also used as a green manure crop because of its high nutrient content resulting partly from symbiosis with nitrogen-fixing bacteria of the genus Rhizobium.In Europe and worldwide, red clover breeding is focused on improving traits like persistency, resistance to biotic and abiotic factors, and forage yield and quality characteristics such as protein quality and stability, fermentable carbohydrate content, and cell wall digestibility. The benefits of protecting plant proteins from degradation in the rumen have been established. Polyphenol oxidase (PPO) enzymes catalyse the aerobic conversion of monophenols to o-phenols and finally to o-quinones (Macheix et al. 1991) and are present in sign...

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“…They are less optimal compared to textbook F 2 populations derived from a F 1 hybrid of two inbred grandparents, since not all the parental polymorphisms are informative in terms of linkage in the progeny. The first red clover linkage map was constructed by Isobe et al [38], and contained 158 RFLP loci. Subsequently, a high density map with 1399 markers consisting of mainly SSRs and RFLPs was published [39].…”

Section: Genetics and Breedingmentioning

confidence: 99%

“…The map of Hermann et al [40] was used in a study of seed yield components, in which 38 QTL were identified. More recently, the maps of Isobe et al [38], Sato et al [39] and Hermann et al [40] were combined with three other maps to generate an integrated map consisting of 1804 markers distributed over the 7 linkage groups and a total length of 836 cM [42]. Two recent papers have described work on QTL directly linked to persistence and its component traits.…”

Section: Genetics and Breedingmentioning

confidence: 99%

Development of Genomic Resources in the Species of Trifolium L. and Its Application in Forage Legume Breeding

2012

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Clovers (genus Trifolium) are a large and widespread genus of legumes. A number of clovers are of agricultural importance as forage crops in grassland agriculture, particularly temperate areas. White clover (Trifolium repens L.) is used in grazed pasture and red clover (T. pratense L.) is widely cut and conserved as a winter feed. For the diploid red clover, genetic and genomic tools and resources have developed rapidly over the last five years including genetic and physical maps, BAC (bacterial artificial chromosome) end sequence and transcriptome sequence information. This has paved the way for the use of genome wide selection and high throughput phenotyping in germplasm development. For the allotetraploid white clover progress has been slower although marker assisted selection is in use and relatively robust genetic maps and QTL (quantitative trait locus) information now exist. For both species the sequencing of the model legume Medicago truncatula gene space is an important development to aid genomic, biological and evolutionary studies. The first genetic maps of another species, subterranean clover (Trifolium subterraneum L.) have also been published and its comparative genomics with red clover and M. truncatula conducted. Next generation sequencing brings the potential to revolutionize clover genomics, but international consortia and effective use of germplasm, novel population structures and phenomics will be required to carry out effective translation into breeding. Another avenue for clover genomic and genetic improvement is interspecific hybridization. This approach has considerable potential with regard to crop improvement but also opens windows of opportunity for studies of biological and evolutionary processes.

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First RFLP linkage map of red clover (Trifolium pratense L.) based on cDNA probes and its transferability to other red clover germplasm

Cited by 42 publications

References 39 publications

Identification of SSR markers using soybean (Glycine max) ESTs from globular stage embryos

Identification of SSR markers using soybean (Glycine max) ESTs from globular stage embryos

Modern methods for genetic improvement of Trifolium pratense.

Development of Genomic Resources in the Species of Trifolium L. and Its Application in Forage Legume Breeding

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