First results on cell irradiation with laser-driven protons on the TARANIS system

Cellular responses to DNA double-strand breaks (DSBs) not only promote genomic integrity in healthy tissues, but also largely determine the efficacy of many DNA-damaging cancer treatments, including X-ray and particle therapies. A growing body of evidence suggests that activation of the mechanisms that detect, signal and repair DSBs may depend on the complexity of the initiating DNA lesions. Studies focusing on this, as well as on many other radiobiological questions, require reliable methods to induce DSBs of varying complexity, and to visualize the ensuing cellular responses. Accelerated particles of different energies and masses are exceptionally well suited for this task, due to the nature of their physical interactions with the intracellular environment, but visualizing cellular responses to particle-induced damage - especially in their early stages - at particle accelerator facilities, remains challenging. Here we describe a straightforward approach for real-time imaging of early response to particle-induced DNA damage. We rely on a transportable setup with an inverted fluorescence confocal microscope, tilted at a small angle relative to the particle beam, such that cells can be irradiated and imaged without any microscope or beamline modifications. Using this setup, we image and analyze the accumulation of fluorescently-tagged MDC1, RNF168 and 53BP1—key factors involved in DSB signalling—at DNA lesions induced by 254 MeV α-particles. Our results provide a demonstration of technical feasibility and reveal asynchronous initiation of accumulation of these proteins at different individual DSBs.

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Laser ion acceleration for hadron therapy

Bulanov¹,

Wilkens²,

Esirkepov³

et al. 2014

Успехи физических наук

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First results on cell irradiation with laser-driven protons on the TARANIS system

Cited by 3 publications

References 6 publications

Dose calculations in a cell monolayer for high-throughput irradiation with proton beams generated by PW lasers for space applications

Dose calculations in a cell monolayer for high-throughput irradiation with proton beams generated by PW lasers for space applications

A simple microscopy setup for visualizing cellular responses to DNA damage at particle accelerator facilities

Laser ion acceleration for hadron therapy

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