First reports of XXY aneuploidy in natural populations of Thomas’ pine vole Microtus thomasi (Rodentia: Arvicolidae) from Greece

Rovatsos, Michail; Mitsainas, George P.; Stamatopoulos, C.; Giagia‐Athanasopoulou, Eva B.

doi:10.1016/j.mambio.2007.10.010

Cited by 11 publications

(17 citation statements)

References 46 publications

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“…atticus with 2n = 38-44 (one individual had 2n = 45 (XXY)) and different sex chromosome constitutions, as described by Rovatsos et al [2008] ( fig. 3 ; table 1 ).…”

Section: Resultsmentioning

confidence: 99%

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Molecular and Physical Characterization of the Complex Pericentromeric Heterochromatin of the Vole Species Microtus thomasi

Rovatsos

Marchal

Romero-Fernández

et al. 2014

Cytogenet Genome Res

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A new repeated DNA from Microtus thomasi, Mth-Alu2.2, was cloned and characterized and is presented here for the first time. Digestion of genomic DNA from M. thomasi with AluI restriction enzyme revealed a 2.2-kb repetitive DNA sequence with a high AT content (69%). This sequence consists of a tandemly repeated nonanucleotide of the consensus sequence CACAATGTA, which constitutes approximately 93-95% of the total unit length. The location of the Mth-Alu2.2 sequence in the karyotype was determined by FISH, demonstrating strong hybridization signals in the pericentromeric regions of all chromosomes and in the heterochromatin blocks of several X chromosome variants. In addition, the distribution of the 4 pericentromeric repeat sequences Msat-160, Mth-Alu900, Mth-Alu2.2, and interstitial telomeric repeats was analyzed by in situ hybridization in M. thomasi, in order to shed light on the complex composition of the chromosomal pericentromeric regions in this species. The order and organization of these sequences in the pericentromeric regions are conserved, with slight variations in both the degree of overlapping and the amount of each repeated DNA in the chromosomes. Specifically, Mth-Alu2.2 is localized in the terminal regions of the chromosomes, with Msat-160 occupying the immediately inner region, partially intermixed with Mth-Alu2.2. The sequence Mth-Alu900 is found in internal positions below Msat-160, and the interstitial telomeric repeats are located close to the long-arm euchromatin of the chromosomes.

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“…atticus with 2n = 38-44 (one individual had 2n = 45 (XXY)) and different sex chromosome constitutions, as described by Rovatsos et al [2008] ( fig. 3 ; table 1 ).…”

Section: Resultsmentioning

confidence: 99%

“…a Refers to the population numbers in figure 1. b Chromosomal races according to Rovatsos [2012]. c This R32 individual has 2 X st1 and 1 Y 1 [Rovatsos et al, 2008].…”

Section: Southern Blotmentioning

confidence: 99%

Molecular and Physical Characterization of the Complex Pericentromeric Heterochromatin of the Vole Species Microtus thomasi

Rovatsos

Marchal

Romero-Fernández

et al. 2014

Cytogenet Genome Res

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“…hybrids between the karyomorphs M. thomasi 'thomasi' and 'atticus' Rovatsos et al, 2008) suggest that these two could be considered as different local populations of the same species. Several studies have demonstrated that amplification of repetitive DNA sequences on sex chromosomes of Microtus species gives rise to blocks of constitutive heterochromatin (Kozlova et al, 2003;Marchal et al, 2004aMarchal et al, , b, 2006Acosta et al, 2008), possibly through different, independent and rapid processes in each species.…”

Section: Mth-alu900-con Actggtcaaaccggttta---------------------------mentioning

confidence: 99%

A new pericentromeric repeated DNA sequence in <i>Microtus thomasi</i>

Acosta

Marchal

Mitsainas

et al. 2009

Cytogenet Genome Res

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Several karyotypic forms have been previously described in populations of the vole species Microtus thomasi from Greece. In particular, the karyomorphs Microtus thomasi ‘thomasi’ and ‘atticus’ differ in X chromosome morphology, being acrocentric and subtelocentric, respectively. Furthermore, remarkable heterochromatin content variability has been described in sex chromosomes of both karyomorphs. Genomic DNA digestion with AluI allowed us to clone an 884 bp long repeated DNA sequence (Mth-Alu900) from the karyomorph M. thomasi ‘atticus’. This repeated DNA is AT rich and seems to be organized mainly as a dimer of the 884-bp unit, which presents three simple repeats (CAAAT, CAGAT and CAGAC) that constitute 80% of the total unit length. This repeated DNA is exclusive to M. thomasi, since it is absent from the genome of other studied Arvicolinae species. The chromosomal location of Mth-Alu900 was analyzed on M. thomasi ‘thomasi’ and M. thomasi ‘atticus’ karyomorphs, with different sex chromosome constitution. It was mainly located on the pericentromeric heterochromatin of most autosomes and X chromosomes on both karyomorphs. Results are also discussed in relation to karyotypic and sex chromosome variations in M. thomasi. To our knowledge, Mth-Alu900 constitutes a new – the third discovered so far – pericentromeric repeated DNA sequence described in Microtus species.

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“…Among other mammals, XXY males have been shown to be particularly frequent in wild Microtus voles; for example, in a total of 630M. arvalis and M. thomasi, there were six sex chromosome aneuploids of which half had the XXY condition (Zima et al 1992;Rovatsos et al 2008). However, unlike the house mouse, both M. arvalis and M. thomasi have asynaptic X-Y pairing during meiosis and this may lead to a high incidence of sex chromosome aneuploidy, including occurrence of XXY individuals.…”

Section: Discussionmentioning

confidence: 99%

“…An XXY karyotype has very rarely been recorded in wild mammals, but there are reports for the tammar wallaby (Macropus eugenii; Sharman et al 1970), common shrew (Sorex araneus; Searle 1984), common vole (Microtus arvalis; Zima et al 1992) and Thomas' pine vole (Microtus thomasi; Rovatsos et al 2008). …”

Section: Introductionmentioning

confidence: 99%

First wild XXY house mice

et al. 2010

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Laboratory house mice (Mus musculus) with the XXY condition can be generated with ease and have been used as a biomedical model. However, although the XXY constitution has been described in humans and many domestic and wild mammal species, and a very large number of wild house mice have been karyotyped previously, no wild individuals of M. musculus with an XXY karyotype have ever been reported. Therefore, it is rather extraordinary that two wild XXY house mice were caught by us on two different farms in northern Italy in 2008. Except for the extra X chromosome, one male had a standard karyotype (2n = 40) and the other, the karyotype of the Cremona metacentric population (2n = 22). In this paper, the phenotype of these two individuals is described. Observations for both of these wild males agree with those of laboratory XXY mice, i.e., they had a normal body mass and appearance, but significantly smaller testes than normal, and no visible germ cells. The incidence of the XXY chromosome anomaly in wild mice (two among 5,123 wild mice surveyed by us and our colleagues, i.e., approximately 0.08% among wild-caught males) is intermediate between that found in male laboratory mice (approximately 0.04%) and that found in male humans (0.2%).

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First reports of XXY aneuploidy in natural populations of Thomas’ pine vole Microtus thomasi (Rodentia: Arvicolidae) from Greece

Cited by 11 publications

References 46 publications

Molecular and Physical Characterization of the Complex Pericentromeric Heterochromatin of the Vole Species Microtus thomasi

Molecular and Physical Characterization of the Complex Pericentromeric Heterochromatin of the Vole Species Microtus thomasi

A new pericentromeric repeated DNA sequence in <i>Microtus thomasi</i>

First wild XXY house mice

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