First report on the molecular identification of the phytoplasma associated with a lethal yellowing‐type disease of coconut palms in Cote d'Ivoire

Konan, Jean Louis; Allou, Kouassi; Diallo, Hortense Atta; Yao, D. Saraka; Koua, B.; Kouassi, N.; Benabid, R.; Michelutti, R.; Scott, James A.; Arocha-Rosete, Yaima

doi:10.5197/j.2044-0588.2013.028.003

Cited by 15 publications

(8 citation statements)

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“…Palmindustrie V1, with 30 000 ha devoted to coconut plantations was the first area in Grand‐Lahou where LY‐like symptoms were observed in the 1990s (Konan Konan et al, ); however, no pathogen (including phytoplasma) was found associated with the disease by that time. In 2012, a severe CILY outbreak was reported and associated with a phytoplasma (Konan Konan et al, ), and at the beginning of 2013, the disease was spotted in the village of Likpilassié, located at 2 km from Palmindustrie V1.…”

Section: Discussionmentioning

confidence: 99%

“…Since the 1990s, a lethal yellowing (LY)‐type disease of coconut severely affected Grand‐Lahou, located in the southern coastal district of the country (Arocha‐Rosete et al, ). It was, however, not until 2013 that the Côte d'Ivoire Lethal Yellowing (CILY) was associated with a phytoplasma (Konan Konan et al, ). The disease has decimated over 400 ha of coconut plantations (Arocha‐ Rosete et al, ), and it is rapidly spreading into more coconut‐growing villages of Grand‐Lahou.…”

Section: Introductionmentioning

confidence: 99%

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Detection and differentiation of the coconut lethal yellowing phytoplasma in coconut‐growing villages of Grand‐Lahou, Côte d'Ivoire

Rosete¹,

Diallo

Konan

et al. 2017

Annals of Applied Biology

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Surveys for the Côte d'Ivoire lethal yellowing (CILY) phytoplasma were conducted in eight severely CILY-affected villages of Grand-Lahou in 2015. Leaves, inflorescences and trunk borings were collected from coconut palms showing CILY symptoms and from symptomless trees. Total DNA was extracted from these samples and tested by nested polymerase chain reaction/RFLP and sequence analysis of the 16S rRNA, ribosomal protein (rp) and the translocation protein (secA) genes. The CILY phytoplasma was detected in 82.9% of the symptom-bearing palms collected from all the surveyed villages and from all the plant parts. Trunk borings were recommended as the most suitable plant tissue type for sampling. Results indicate that the CILY phytoplasma may have a westward spread to other coconut-growing areas of Grand-Lahou. CILY phytoplasma strains infecting coconut palms in the western region of Grand-Lahou exhibited unique single nucleotide polymorphisms on the rp sequence compared to the strains from the eastern region. Moreover, single nucleotide polymorphisms on the SecA sequence distinguished the CILY phytoplasma from the Cape St. Paul Wilt Disease phytoplasma in Ghana, and the Lethal Yellowing phytoplasma in Mozambique. (Résumé d'auteur

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Detection and differentiation of the coconut lethal yellowing phytoplasma in coconut‐growing villages of Grand‐Lahou, Côte d'Ivoire

Rosete¹,

Diallo

Konan

et al. 2017

Annals of Applied Biology

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“…Côte d"Ivoire is among the top 20 coconut producers in the world, with an annual production of 182,170 tonnes (FAOSTAT, 2015), and it is the top African exporter of coconut oil from copra to Europe and West Africa. However, the Ivoirian coconut industry is currently impacted by the Côte d"Ivoire lethal yellowing disease (CILY) (Konan Konan et al, 2013a). Lethal yellowing-like diseases are considered a serious world threat for coconut-producing countries in Central America and the Caribbean, West and East Africa, and Mozambique (Danyo, 2011).…”

Section: Introductionmentioning

confidence: 99%

Microbial diversity in leaves, trunk and rhizosphere of coconut palms (Cocos nucifera L.) associated with the coconut lethal yellowing phytoplasma in Grand-Lahou, Cte dIvoire

Morales-Lizcano¹,

Hasan²,

To³

et al. 2017

Afr. J. Biotechnol.

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The diversity of culturable bacterial and fungal communities was assessed from leaves, trunk and rhizosphere of coconut palms infected and non-infected by the Côte d'Ivoire lethal yellowing (CILY) phytoplasma. Bacterial and fungal microbes were isolated from leaves, trunk and rhizosphere samples collected from two villages of Grand-Lahou, Braffedon and Yaokro, by using a high-throughput 'dilution-to-extinction' cultivation method coupled with PCR and sequencing with primers that amplified both the 16S ribosomal RNA and intergenic transcribed spacer (ITS) genes. The relative abundance was higher for Bacillus and Candida in Braffedon, and Burkholderia and Neodeightonia in Yaokro. Commonly genera identified from rhizosphere included Bacillus, Burkholderia, Pseudomonas, Streptomyces, Cryptococcus, Penicillium, Purpureocillium and Trichoderma. The most abundant endophytes identified were Pantoea, Candida, Cryptococcus, Bacillus, Pseudomonas, Penicillium, Aspergillus and Rhodotorula. Genera limited to symptomless palms included Arthrininum, Chaetomium, Phialemonium, Fusarium, Klebsiella and Candida. Results indicate that the CILY phytoplasma may be a factor determining the level of diversity of a microbial community in a given location. Our research provides the basis to investigate the possible effect of endophytic and rhizosphere microbes against the CILY phytoplasma to further effectively improve the management of CILY in Grand-Lahou.

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“…In West Africa, these diseases are known by various local or regional names. They include Awka wilt disease in Nigeria (Ekpo & Ojomo, 1990), Cape St Paul wilt (CSPW) disease in Ghana (Ofori & Nkansah-Poku, 1997) and Côte d’Ivoire (Konan Konan et al , 2013), Kaïncopé in Togo (Dabek et al , 1976) and Kribi disease in Cameroun (Dollet et al , 1977). In East Africa, a lethal disease of coconut is present along the coastal belt of Tanzania and in Kenya (Schuiling & Mpunami, 1992), while a similar disease is active in Mozambique (Bonnot et al , 2010).…”

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confidence: 99%

“…Coconut palms with symptoms indicative of LYD were reported near Quelimane in Zambesia Province, Mozambique, during 2007 (Dollet et al , 2009) and, in 2013, were observed for the first time in the Grand Lahou district on the southern coastal belt of Côte d’Ivoire (Konan Konan et al , 2013). Although the coconut-infecting phytoplasmas detected in both countries were judged most similar to strains previously associated with Awka wilt disease in Nigeria and CSPW disease in Ghana (Dollet et al , 2011; Konan Konan et al , 2013), their precise taxonomic positions and group affiliations were not determined. In this communication, we report results from nucleotide and phylogenetic analysis of 16S rRNA gene sequences, and propose that phytoplasma strains associated with coconut LYD in Mozambique, Nigeria, Ghana and Côte d’Ivoire represent a distinct species-level lineage and novel taxon, ‘ Candidatus Phytoplasma palmicola’.…”

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‘Candidatus Phytoplasma palmicola’, associated with a lethal yellowing-type disease of coconut (Cocos nucifera L.) in Mozambique

Harrison

Davis

Oropeza

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

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In this study, the taxonomic position and group classification of the phytoplasma associated with a lethal yellowing-type disease (LYD) of coconut (Cocos nucifera L.) in Mozambique were addressed. Pairwise similarity values based on alignment of nearly full-length 16S rRNA gene sequences (1530 bp) revealed that the Mozambique coconut phytoplasma (LYDM) shared 100 % identity with a comparable sequence derived from a phytoplasma strain (LDN) responsible for Awka wilt disease of coconut in Nigeria, and shared 99.0–99.6 % identity with 16S rRNA gene sequences from strains associated with Cape St Paul wilt (CSPW) disease of coconut in Ghana and Côte d’Ivoire. Similarity scores further determined that the 16S rRNA gene of the LYDM phytoplasma shared <97.5 % sequence identity with all previously described members of ‘Candidatus Phytoplasma ’. The presence of unique regions in the 16S rRNA gene sequence distinguished the LYDM phytoplasma from all currently described members of ‘Candidatus Phytoplasma ’, justifying its recognition as the reference strain of a novel taxon, ‘Candidatus Phytoplasma palmicola’. Virtual RFLP profiles of the F2n/R2 portion (1251 bp) of the 16S rRNA gene and pattern similarity coefficients delineated coconut LYDM phytoplasma strains from Mozambique as novel members of established group 16SrXXII, subgroup A (16SrXXII-A). Similarity coefficients of 0.97 were obtained for comparisons between subgroup 16SrXXII-A strains and CSPW phytoplasmas from Ghana and Côte d’Ivoire. On this basis, the CSPW phytoplasma strains were designated members of a novel subgroup, 16SrXXII-B.

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First report on the molecular identification of the phytoplasma associated with a lethal yellowing‐type disease of coconut palms in Cote d'Ivoire

Cited by 15 publications

References 1 publication

Detection and differentiation of the coconut lethal yellowing phytoplasma in coconut‐growing villages of Grand‐Lahou, Côte d'Ivoire

Detection and differentiation of the coconut lethal yellowing phytoplasma in coconut‐growing villages of Grand‐Lahou, Côte d'Ivoire

Microbial diversity in leaves, trunk and rhizosphere of coconut palms (Cocos nucifera L.) associated with the coconut lethal yellowing phytoplasma in Grand-Lahou, Cte dIvoire

‘Candidatus Phytoplasma palmicola’, associated with a lethal yellowing-type disease of coconut (Cocos nucifera L.) in Mozambique

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