First Report on Natural Infection of Phlebotomus sergenti with Leishmania tropica in a Classical Focus of Leishmania major in Tunisia

Jaouadi, Kaouther; Bettaieb, Jihéne; Bennour, Amira; Salem, Sadok; Rjeibi, Mohamed Ridha; Châabane, Sana; Yazidi, Rihab; Khabouchi, Neila; Gharbi, Alya; Salah, Afif Ben

doi:10.4269/ajtmh.16-0849

Cited by 5 publications

(7 citation statements)

References 15 publications

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“…These lesions are distinguished by their chronicity and long duration of development [37,38]. Its causal agent, L. tropica [27,39] Figure 2) [47,48] and in human cases [47] of the same region confirms the vectorial role of this species in the transmission of L. tropica in Tunisia. Indeed, P. sergenti is the proven vector of L. tropica in the Middle East and Morocco [14,42].…”

Section: Leishmaniasis Due To L Tropica Zymodeme Mon-08mentioning

confidence: 87%

The current status of phlebotomine sandflies (Diptera: Psychodidae) in Tunisia and their role on Leishmania transmission: A review

Tabbabi¹,

Sboui²,

Daaboub³

2017

APJTD

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Section: Leishmaniasis Due To L Tropica Zymodeme Mon-08mentioning

confidence: 87%

The current status of phlebotomine sandflies (Diptera: Psychodidae) in Tunisia and their role on Leishmania transmission: A review

Tabbabi¹,

Sboui²,

Daaboub³

2017

APJTD

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“…There is need for novel molecular tools to respond to challenges associated to leishmaniases and continuous changes in their epidemiology at local and global levels [12,20,21,66]. Indeed, molecular tools are instrumental to define strategies for disease control as they allow PLOS NEGLECTED TROPICAL DISEASES alternative of choice to laborious, costly and time consuming MLEE methods [25,67,68].…”

Section: Discussionmentioning

confidence: 99%

Dipeptidyl peptidase III as a DNA marker to investigate epidemiology and taxonomy of Old World Leishmania species

et al. 2021

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Background Dipeptidyl peptidase III (DPPIII) member of M49 peptidase family is a zinc-dependent metallopeptidase that cleaves dipeptides sequentially from the N-terminus of its substrates. In Leishmania, DPPIII, was reported with other peptidases to play a significant role in parasites’ growth and survival. In a previous study, we used a coding sequence annotated as DPPIII to develop and evaluate a PCR assay that is specific to dermotropic Old World (OW) Leishmania species. Thus, our objective was to further assess use of this gene for Leishmania species identification and for phylogeny, and thus for diagnostic and molecular epidemiology studies of Old World Leishmania species. Methodology Orthologous DDPIII genes were searched in all Leishmania genomes and aligned to design PCR primers and identify relevant restriction enzymes. A PCR assays was developed and seventy-two Leishmania fragment sequences were analyzed using MEGA X genetics software to infer evolution and phylogenetic relationships of studied species and strains. A PCR-RFLP scheme was also designed and tested on 58 OW Leishmania strains belonging to 8 Leishmania species and evaluated on 75 human clinical skin samples. Findings Sequence analysis showed 478 variable sites (302 being parsimony informative). Test of natural selection (dN-dS) (-0.164, SE = 0.013) inferred a negative selection, characteristic of essential genes, corroborating the DPPIII importance for parasite survival. Inter- and intra-specific genetic diversity was used to develop universal amplification of a 662bp fragment. Sequence analyses and phylogenies confirmed occurrence of 6 clusters congruent to L. major, L. tropica, L. aethiopica, L. arabica, L. turanica, L. tarentolae species, and one to the L. infantum and L. donovani species complex. A PCR-RFLP algorithm for Leishmania species identification was designed using double digestions with HaeIII and KpnI and with SacI and PvuII endonucleases. Overall, this PCR-RFLP yielded distinct profiles for each of the species L. major, L. tropica, L. aethiopica, L. arabica and L. turanica and the L. (Sauroleishmania) L. tarentolae. The species L. donovani, and L. infantum shared the same profile except for strains of Indian origin. When tested on clinical samples, the DPPIII PCR showed sensitivities of 82.22% when compared to direct examination and was able to identify 84.78% of the positive samples. Conclusion The study demonstrates that DPPIII gene is suitable to detect and identify Leishmania species and to complement other molecular methods for leishmaniases diagnosis and epidemiology. Thus, it can contribute to evidence-based disease control and surveillance.

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“…Nevertheless, Sergentomyia is believed to transmit lizard Leishmania species whereas Phlebotomus transmit human pathogenic species of Leishmania [30]. However, studies in Ghana and elsewhere have suggested the possible involvement of Sergentomyia minuta, Sergentomyia ingrami, Sergentomyia hamoni and Sergentomyia darlingi as vectors of leishmaniasis [29,[31][32][33]. This reiterating the fact that some Sergentomyia species disclose periodic or opportunistic anthropophilic feeding behaviour.…”

Section: Discussionmentioning

confidence: 99%

Identification of Blood Meal of Sand Flies in a Cutaneous Leishmaniasis Endemic Area, Volta Region-Ghana

Doe¹,

Kwakye-Nuako²,

Egyir-Yawson³

2020

AJBLS

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Background: Vector incrimination is of utmost importance in the transmission of Leishmania species identified in the Volta region of Ghana. The feeding preference of sand flies provides valuable information about the vector-host interactions, including reservoir host. Objective: The main aim of this study was to identify the blood meal in collected sand flies and its source from three villages in a cutaneous leishmaniasis endemic area in the Ho Municipality. Method: Sand flies was collected using CDC light straps and sticky paper traps from Lume Atsiame, Dodome Dogblome and Dodome Awuiasu. They were morphologically identified by taxonomic keys. DNA was extracted from the individual sand flies using the potassium acetate extraction method. Polymerase chain reaction (PCR) amplification of cytochrome b gene (cyt b) fragment was carried out and subsequently DNA sequenced. Results: Of a total of three hundred and sixty-three (363) female sand flies that were analysed, eighty-four (84) representing 23.14% were positive for blood meal presence with an amplified DNA of the expected size, 359bp. Out of the number that was sequenced, the cyt b sequencing revealed that four (4) blood fed females fed on bloodmeal from human (Homo sapiens) and house mouse (Mus musculus). Conclusion: The blood meal presence was more predominant in Sergentomyia ghesquierei and least in Sergentomyia schwetzi. This may aid in the development of effective strategies to control cutaneous leishmaniasis in the endemic areas.

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First Report on Natural Infection of Phlebotomus sergenti with Leishmania tropica in a Classical Focus of Leishmania major in Tunisia

Cited by 5 publications

References 15 publications

The current status of phlebotomine sandflies (Diptera: Psychodidae) in Tunisia and their role on Leishmania transmission: A review

The current status of phlebotomine sandflies (Diptera: Psychodidae) in Tunisia and their role on Leishmania transmission: A review

Dipeptidyl peptidase III as a DNA marker to investigate epidemiology and taxonomy of Old World Leishmania species

Identification of Blood Meal of Sand Flies in a Cutaneous Leishmaniasis Endemic Area, Volta Region-Ghana

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