First Report on Molecular Characterization of Taenia multiceps Isolates From Sheep and Goats in Faisalabad, Pakistan

Alvi, Mughees Aizaz; Ohiolei, John Asekhaen; Saqib, Muhammad; Tayyab, Muhammad; Khan, Muhammad Umar Zafar; Li, Li; Aqib, Amjad Islam; Ali, Hassan; Alvi, Anum Aizaz; Qamar, Warda; Fu, Bao‐Quan; Yan, Hong‐Bin; Jia, Wan‐Zhong

doi:10.3389/fvets.2020.594599

Cited by 3 publications

(4 citation statements)

References 55 publications

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“…In Asia, enzootic coenurosis infections have been reported from India (2.9% in sheep) [ 49 ], Iran (1.7–18.6% in sheep and goats) [ 29 , 50 – 53 ], Bangladesh (2.5% in calves and 5% in Beetal goats) [ 54 , 55 ], Pakistan (0.4% in sheep and goats) [ 56 ], Japan [ 57 ] and Russia [ 58 ]. In the Middle East, cerebral coenurosis is a major endemic disease affecting small ruminants, most notably in Turkey, Egypt, Iraq, and Jordan [ 59 , 60 ], with prevalences of 2.9% in Jordanian sheep [ 61 ], 22.8–23.68% in Iraqi sheep and goats [ 62 , 63 ], 1.3–28.5% in Turkish sheep [ 64 , 65 ], and 16.6% in Dafuri goats in Oman [ 59 ].…”

Section: Geographical Distribution and Epidemiologymentioning

confidence: 99%

“…The significance and extent of intraspecific variation within certain Taenia species are unknown. A thorough understanding of cestode genetic variability and population dynamics is crucial for the effective implementation of prevention and control strategies, as they provide insight into host specialization and regional peculiarities [ 30 , 56 ]. The first indication of genetic variation in T. multiceps was from an study on Italian sheep [ 77 ], where the genetic variants Tm1, Tm2 and Tm3 were identified using cytochrome c oxidase subunit 1 ( cox1 ) and nicotinamide adenine dinucleotide dehydrogenase subunit 1 ( nad1 ) analysis, with Tm1 being the most frequent variant identified.…”

Section: Molecular Characterizationmentioning

confidence: 99%

“…In contrast, T. multiceps isolates from sheep and goats in Iran displayed a significant degree of genetic heterogeneity, with the cox1 gene exhibiting 11 segregation sites, resulting in seven haplotypes in sheep [ 3 , 22 ]. Furthermore, a study from Pakistan [ 56 ] suggested the existence of unique T. multiceps haplotypes with high haplotype diversity, but low nucleotide diversity, in sheep and goats through a phylogenetic analysis that placed Pakistani and Chinese isolates in a cluster at a reasonable distance from another cluster comprising isolates from other countries.…”

Section: Molecular Characterizationmentioning

confidence: 99%

“…The cox1 and nad1 mitochondrial genes are particularly useful for the identification of Taenia gaigeri and Taenia skrjabini , which were previously assumed to be the causal agents of non-cerebral coenurosis in goats and sheep, respectively, but are now regarded as genetic variants of Taenia multiceps [ 28 , 56 ]. Indeed, a study carried out in the United Arab Emirates using isolates obtained from subcutaneous and muscular coenuri from goats [ 28 ] revealed significant differences from other cox1 sequence deposited in GenBank, implying the occurrence of different genotypes or strains of T. multiceps in this intermediate host.…”

Section: Molecular Characterizationmentioning

confidence: 99%

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Taenia multiceps coenurosis: a review

et al. 2022

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Taenia multiceps is a taeniid cestode that inhabits the small intestines of both wild and domestic carnivores. The larval stage, Coenurus cerebralis, is typically found in the central nervous system (CNS) of a wide range of livestock and, to a lesser extent, in the extra-cerebral tissues of sheep and goats. This review covers all aspects of the life cycle of T. multiceps and its epidemiology, molecular characterization, pathogenesis, diagnosis, therapy, control and zoonotic potential. Coenurosis caused by the larval stage of T. multiceps has a worldwide distribution and is often fatal in intermediate hosts, which can result in substantial economic losses in livestock farming. Molecular characterization using the mitochondrial genes cytochrome c oxidase subunit 1 and nicotinamide adenine dinucleotide dehydrogenase subunit 1 of different T. multiceps populations has revealed significant genetic variation and the presence of three major haplotypes. The disease mostly affects young sheep and is referred to as either acute or chronic coenurosis. Acute coenurosis occurs as a result of oncospheres migrating through the CNS, while chronic coenurosis occurs as a consequence of the coenurus maturing, which causes displacement and pressure atrophy of brain tissue. Non-cerebral coenurosis has been most commonly reported in goats. The best diagnostic method for cerebral coenurosis involves the interpretation of clinical signs with accurate localization of the cyst using diagnostic imaging techniques. A vaccine based on recombinant oncosphere antigens has proved to be an effective tool against T. multiceps infection in sheep. Additionally, use of anthelmintics during the parasite’s migration stages reduces the development of cysts in the sheep brain. Surgery is considered the most effective method for the treatment of cerebral coenurosis in small ruminants, but is often not carried out because of the limited finances of many sheep and goat breeders. However, coenurosis can also be controlled effectively through preventative measures, such as anthelmintic treatment of dogs and the proper disposal of intermediate host carcasses. The parasite is also zoonotic, and cases of coenurosis have been reported in humans with coenuri located in the brain, spinal cord and eyes. Graphical abstract

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Section: Geographical Distribution and Epidemiologymentioning

confidence: 99%

Section: Molecular Characterizationmentioning

confidence: 99%

Section: Molecular Characterizationmentioning

confidence: 99%

Section: Molecular Characterizationmentioning

confidence: 99%

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Taenia multiceps coenurosis: a review

et al. 2022

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Hydatigera taeniaeformis in urban rats (Rattus rattus) in Faisalabad, Pakistan

Alvi

Ohiolei

et al. 2021

Infection, Genetics and Evolution

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Molecular characterization and phylogenetic studies of Echinococcus granulosus and Taenia multiceps coenurus cysts in slaughtered sheep in Saudi Arabia

Malki

Hussien

2021

Open Life Sciences

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Taeniids, consisting of two genera Echinococcus and Taenia, are obligatory tapeworms of mammals, and their pathogenicity was due to infection with larval stages. Hydatid (the larval stage of Echinococcus granulosus) and coenurus (the larval stage of Taenia multiceps) cysts are prevalent in domestic, wild ruminants, livestock, swine, and dogs, and accidentally they could also be found in humans. They lead to different clinical manifestations that cause economic loss in livestock and human morbidity. In Saudi Arabia, few studies were performed on hydatid and coenurus cyst genetic variations. The main goal of the present study was to identify E. granulosus and T. multiceps cyst isolates collected from slaughtered Harri sheep in Saudi Arabia by partial sequencing with PCR amplification of the cytochrome C oxidase 1 (COX1) gene. Molecular and phylogenetic evaluation based on COX1 sequences indicated that cyst isolates belong to E. granulosus and T. multiceps, respectively, successfully submitted in NCBI Genbank. Molecular characterization showed a low nucleotide diversity with two submitted isolates of coenurus with related isolates of Genbank. Conversely, E. granulosus isolates showed higher nucleotide diversity. The reported data could serve as a foundation for future molecular epidemiological and biological studies.

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First Report on Molecular Characterization of Taenia multiceps Isolates From Sheep and Goats in Faisalabad, Pakistan

Cited by 3 publications

References 55 publications

Taenia multiceps coenurosis: a review

Taenia multiceps coenurosis: a review

Hydatigera taeniaeformis in urban rats (Rattus rattus) in Faisalabad, Pakistan

Molecular characterization and phylogenetic studies of Echinococcus granulosus and Taenia multiceps coenurus cysts in slaughtered sheep in Saudi Arabia

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