First report on Enterocytozoon hepatopenaei (EHP) infection in Pacific white shrimp (Penaeus vannamei) cultured in Korea

Kim, Ji Hyung; Lee, Cho-Rong; Jeon, Hye Jin; Kim, Bum-Keun; Lee, NamKyung; Choi, Seong‐Kyoon; Han, Jee Eun

doi:10.1016/j.aquaculture.2021.737525

Cited by 20 publications

(13 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…During monitoring for effective disease control and prevention, EHP was identified in clinically healthy giant freshwater prawns using histopathological and molecular biological analyses. In the Republic of Korea, since the first report of EHP infection in Pacific whiteleg shrimp in Ganghwa-gun in 2020 [ 18 ], EHP has been detected in various regions [ 19 ].…”

Section: Discussionmentioning

confidence: 99%

“…The lack of any drug treatment for HPM [ 8 , 16 ], transmission of various surrounding organisms [ 8 , 9 , 11 ], and spread in several regions [ 19 ] make it difficult to effectively treat the disease upon occurrence. Therefore, periodic monitoring should be performed preemptively to control and prevent EHP transmission.…”

Section: Discussionmentioning

confidence: 99%

“…Black tiger shrimp ( Penaeus monodon ), Pacific whiteleg shrimp ( Litopenaeus vannamei ), and blue shrimp ( Litopenaeus stylirostris ) were deemed susceptible host species against HPM [ 1 , 12 , 13 ]. Vietnam, India, Brunei, China, Indonesia, Malaysia, Venezuela, Australia, and the Republic of Korea were reported as countries where HPM infection most commonly occurred [ 13 , 14 , 15 , 16 , 17 , 18 , 19 ]. In the Republic of Korea, EHP was first isolated from Pacific whiteleg shrimp in April 2020 [ 18 ] and has been reported in multiple instances ever since [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

“…Vietnam, India, Brunei, China, Indonesia, Malaysia, Venezuela, Australia, and the Republic of Korea were reported as countries where HPM infection most commonly occurred [ 13 , 14 , 15 , 16 , 17 , 18 , 19 ]. In the Republic of Korea, EHP was first isolated from Pacific whiteleg shrimp in April 2020 [ 18 ] and has been reported in multiple instances ever since [ 19 ]. However, there are no reports of EHP infection in other shrimp or prawns in the Republic of Korea.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

First Report of Enterocytozoon hepatopenaei Infection in Giant Freshwater Prawn (Macrobrachium rosenbergii de Man) Cultured in the Republic of Korea

Jang

Kim

et al. 2022

Animals

View full text Add to dashboard Cite

In the Republic of Korea, Enterocytozoon hepatopenaei (EHP) was first isolated from Pacific whiteleg shrimp in April 2020; however, there are no existing reports of EHP infection in other shrimp or prawns. Here, we aimed to investigate EHP infection and its prevalence in giant freshwater prawn farms in the Republic of Korea. We tested prawns from 22 farms for EHP infection, and samples from eight farms showed positive EHP infection results in 2021. In EHP-infected prawn farms, the prevalence ranged from 4.9% to 18.2%. The prevalence of EHP infection in the Republic of Korea, derived from the prevalence in prawn farms, was estimated to be 0.8% in 2021. The proliferation of EHP was observed within the hepatopancreatic epithelial cells of prawns using H&E and Giemsa staining. Mature EHP was observed in the sinus between epithelial cells of the digestive tubules. Phylogenetic analysis revealed a clade distinct from the previously reported EHP in Pacific whiteleg shrimps. This is the first report of EHP infection in a giant freshwater prawn in the Republic of Korea, where the prevalence of EHP infection is not high, but it is recognized as an emerging disease that requires periodic monitoring and quarantine management in giant freshwater prawns.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

First Report of Enterocytozoon hepatopenaei Infection in Giant Freshwater Prawn (Macrobrachium rosenbergii de Man) Cultured in the Republic of Korea

Jang

Kim

et al. 2022

Animals

View full text Add to dashboard Cite

show abstract

“…EHP has a wide range of hosts, such as Litopenaeus vannamei, Penaeus monodon, and P. stylirostris (Tang et al, 2015;Chaijarasphong et al, 2021). The incidence of EHP has been reported to be high in major shrimp farming species such as L. vannamei and P. monodon in Asian countries, including China, Indonesia, Malaysia, Vietnam, Thailand, and India (Kim et al, 2022), causing huge economic losses to the shrimp farming industry every year (Rajendran et al, 2016;Shen et al, 2019;Aranguren Caro et al, 2020;Patil et al, 2021). Therefore, a variety of effective measures must be implemented to address the current critical situation.…”

Section: Introductionmentioning

confidence: 99%

Rapid visual detection of Enterocytozoon hepatopenaei by recombinase polymerase amplification combined with lateral flow dipstick

Pang

Zhao

et al. 2022

Front. Mar. Sci.

View full text Add to dashboard Cite

Enterocytozoon hepatopenaei (EHP) is a high-impact pathogen in shrimp farming, causing huge economic losses to the global shrimp farming industry every year. However, current EHP detection methods are primarily based on the development of polymerase chain reaction (PCR) techniques that rely on sophisticated and expensive instruments. Consequently, a rapid, practical, and sensitive protocol for the detection of EHP is necessary. Recombinase polymerase amplification combined with a lateral flow dipstick (LFD-RPA) assay was developed using a pair of primers and nfo-probe targeting the conserved region of the spore wall protein gene. Under optimized reaction conditions, the LFD-RPA assay can detect 10 copies/mL of standard plasmid within 20 min at 40°C. Furthermore, the specificity of the LFD-RPA was also verified with other common pathogens of shrimp. Thirty-nine samples of Litopenaeus vannamei were collected in shrimp farms and detected using LFD-RPA and nested PCR. Thirty-two positive samples were detected by LFD-RPA. Compared with those of nested PCR, the diagnostic sensitivity and specificity of LFD-RPA were 100% and 100%, respectively. These results indicated the great application potential of the newly developed LFD-RPA assay for point-of-care diagnosis, epidemic surveillance, and epidemiological investigation of EHP.

show abstract

Prevalence of enterocytozoon hepatopenaei (EHP) in Penaeus monodon farms in India

Rajeshwar,

Kumar,

Jithendran

et al. 2024

Aquacult Int

View full text Add to dashboard Cite

First report on Enterocytozoon hepatopenaei (EHP) infection in Pacific white shrimp (Penaeus vannamei) cultured in Korea

Cited by 20 publications

References 24 publications

First Report of Enterocytozoon hepatopenaei Infection in Giant Freshwater Prawn (Macrobrachium rosenbergii de Man) Cultured in the Republic of Korea

First Report of Enterocytozoon hepatopenaei Infection in Giant Freshwater Prawn (Macrobrachium rosenbergii de Man) Cultured in the Republic of Korea

Rapid visual detection of Enterocytozoon hepatopenaei by recombinase polymerase amplification combined with lateral flow dipstick

Prevalence of enterocytozoon hepatopenaei (EHP) in Penaeus monodon farms in India

Contact Info

Product

Resources

About