First Report of Viral Nervous Necrosis in Asian Sea Bass, Lates Calcarifer Cultured in Sri Lanka

Abstract

Viral Nervous Necrosis (VNN) caused by Betanodavirus is a devastating disease in aquatic animals. The virus infects both marine and freshwater fish worldwide. Vacuolating necrosis of neural cells of the brain, the retina of the eye, and the spinal cord of the infected fish are the primary histological lesions of the condition. It causes up to 100% mortality in larvae and juvenile fish and can cause significant death in adult fish. The present study detected viral nervous necrosis in larvae and fry of Asian sea bass (Lates calcarifer) with progressive mortality of up to 95% in one week during the Northeast monsoon when the mean water temperature was 27 to 29°C. Histopathological examination of the moribund fish revealed extensive vacuolation and gliosis in the olfactory bulb, the optic lobe of the forebrain, and the inner and outer layer of the retina. Furthermore, tissues of the brain and the retina had intracellular inclusion bodies suggesting viral etiology, further justified by the negative results of the bacterial and parasitic examinations. The Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) test additionally confirms the etiology diagnosis using specific primers designed previously. The histopathology and RT-PCR results suggest that the mortalities of Asian sea bass were due to the VNN. The present finding is the first report of the VNN associated with mass mortalities in Asian seabass cultured in Sri Lanka. These crucial findings emphasize the need for quarantine and control strategies to prevent the spread of the virus and outbreak of the disease.