First report of Tunisian coastal water contamination by protozoan parasites using mollusk bivalves as biological indicators

Ghozzi, Khémissa; Marangi, Marianna; Papini, Roberto Amerigo; Lahmar, I.; Challouf, Rafika; Houas, Najoua; Dhiab, Rym Ben; Normanno, G.; Babba, Hamouda; Giangaspero, Annunziata

doi:10.1016/j.marpolbul.2017.01.057

Cited by 45 publications

(34 citation statements)

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“…In this context, we should not neglect the maritime domain since a seroprevalence among fishermen of 80% was found; this seems to be an additional source of contamination for the coastal region of Monastir. High levels of T. gondii has been demonstrated in some aquatic animals [ 24 ]; the consumption of contaminated mussels [ 17 ] could represent an additional risk factor that should be taken into consideration. Previous studies indicated that T. gondii oocysts may be transported by the flow of freshwater into the ocean [ 6 ], which represents a source of contamination of mussels [ 7 ], and this may partly explain the high prevalence of T. gondii infections in coastal provinces.…”

Section: Discussionmentioning

confidence: 99%

Seroprevalence of Toxoplasma gondii among healthy blood donors in two locations in Tunisia and associated risk factors

et al. 2020

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Toxoplasma gondii is a protozoan parasite that can be transmitted to humans through a variety of routes including blood transfusion. This study aimed to investigate the seroprevalence of T. gondii infection and associated risk factors in healthy blood donors in Tunisia. A total of 800 healthy blood donors from two blood centers in south and coastal Tunisia were analyzed for anti-T. gondii IgG and IgM antibodies by indirect immunofluorescence assay (IFA) and enzyme-linked immunoassays (ELISA), respectively. Structured questionnaires were used to gather information on risk factors for T. gondii infection during collection. The overall seroprevalence was 44.4% of which 352 (44%) and 3 (0.4%) were positive for IgG and both IgG and IgM anti-T. gondii antibodies, respectively. Multivariate analysis showed that T. gondii seropositivity was significantly associated with the birth place (adjusted odds ratio [OR] = 2.72; 95% confidence interval [CI]: 1.49–4.94) and the age of the donors (adjusted OR = 4.98; 95% CI: 1.50–16.58) which are independent risk factors. In addition, the variables of hand washing before eating (adjusted OR = 0.52; 95% CI: 0.37–0.74) and living in an urban environment (adjusted OR = 0.30; 95% CI: 0.13–0.71) are two protective factors. This study provided the first data on the seroprevalence and epidemiology of T. gondii infection in healthy blood donors in Tunisia.

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Section: Discussionmentioning

confidence: 99%

Seroprevalence of Toxoplasma gondii among healthy blood donors in two locations in Tunisia and associated risk factors

et al. 2020

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“…(Moreno et al 2018). Shellfishes are also at risk due to their production in potentially contaminated waters (Tei et al 2016;Ghozzi et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of propidium monoazide–based qPCR to detect viable oocysts of Toxoplasma gondii

et al. 2019

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Information on the viability of Toxoplasma gondii oocysts is crucial to establish the public health significance of this environmental transmission stage that can contaminate water and foods. Interest for molecular-based methods to assess viability is growing and the aim of our study was to assess, for the first time, a propidium monoazide (PMA)-qPCR approach to determine the viability of T. gondii oocysts. Untreated and heat-killed (99°C, 5 min) oocysts were incubated with PMA, a photoreactive DNA binding dye, and analyzed by confocal microscopy and flow cytometry to characterize oocysts' dye permeability. Different PMA concentrations (50 to 150 μM), incubation temperatures (22, 37, and 45°C), amplicon length, selected targeted gene, and dyes (PMA, PMAxx™) were evaluated to define optimal conditions to discriminate specifically viable oocysts by PMA-qPCR. In theory, PMA binding to DNA would inhibit PCR amplification in dead but not in viable oocysts. Incubation at 22°C with 100 μM PMA coupled to qPCR targeting a 123-bp sequence of the 529-bp repeat element allowed the distinction between viable and heated oocysts. However, the reduction of viability following heating of oocysts at high temperature was slight and, contrarily to reverse transcriptase-qPCR, the qPCR signal was not totally suppressed in heated suspensions. Therefore, PMA-qPCR is able to assess the impact of heating on T. gondii oocysts' viability but underestimates the efficacy of this treatment. The relevance of this technique to evaluate the efficacy of other inactivation processes and assess exposure of humans to this pathogen requires further investigations.

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“…El protozoario más importante en la casuística de patología gastrointestinal, como lo es Giardia spp, se encontró en un 30%. Este parásito se ha reportado en mariscos de países asiáticos, europeos y norteamericanos (Robertson, 2007;Gómez-Couso & Ares-Mazás, 2012;Oliveira et al, 2016;Ghozzi et al, 2017;Ligda, 2019;Tedde et al, 2019) como así también Santos et al (2018) lo han reportado en Brasil. Nuestros resultados son coincidentes con los reportados en otros países y constituyó el primer reporte de Giardia spp en M. edulis en Argentina (Torrecillas et al, 2019).…”

Section: Confirmación De Quistes Deunclassified

“…Existen reportes sobre la presencia de parásitos en moluscos bivalvos tales como Mytilus galloprovincialis, Oestrea edulis, Mytilus edulis, entre otros, que acumulan ooquistes de Cryptosporidium spp y quistes de Giardia spp en otros lugares del mundo (Oliveira et al, 2016;Ghozzi et al, 2017;Tedde et al, 2019). Existen trabajos previos en Argentina, donde se ha reportado la presencia de parásitos de la familia Gymnophalydae que presentan potencial zoonótico en bivalvos de otras regiones (Vázquez et al, 2018).…”

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Avaliação De Alterações De Linha De Costa a Partir Da Ferramenta De Imagens Históricas Do Google Earth: O Caso Da Praia De Itacuruçá (Mangaratiba (Rj) – Brasil)

Abude¹,

Souza²

2020

Oceanografía: Desvelando La Belleza, Los Misterios Y Los Desafíos Del Mar

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Todo o conteúdo deste livro está licenciado sob uma Licença de Atribuição Creative Commons. Atribuição 4.0 Internacional (CC BY 4.0). O conteúdo dos artigos e seus dados em sua forma, correção e confiabilidade são de responsabilidade exclusiva dos autores. Permitido o download da obra e o compartilhamento, desde que sejam atribuídos créditos aos autores, e sem a possibilidade de alterá-la de nenhuma forma ou utilizá-la para fins comerciais.

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First report of Tunisian coastal water contamination by protozoan parasites using mollusk bivalves as biological indicators

Cited by 45 publications

References 44 publications

Seroprevalence of Toxoplasma gondii among healthy blood donors in two locations in Tunisia and associated risk factors

Seroprevalence of Toxoplasma gondii among healthy blood donors in two locations in Tunisia and associated risk factors

Evaluation of propidium monoazide–based qPCR to detect viable oocysts of Toxoplasma gondii

Avaliação De Alterações De Linha De Costa a Partir Da Ferramenta De Imagens Históricas Do Google Earth: O Caso Da Praia De Itacuruçá (Mangaratiba (Rj) – Brasil)

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