First Report of Three Tylenchidae Taxa from Southern Alberta, Canada

Abstract

Members of the family Tylenchidae are highly abundant in soil habitats, including agricultural settings, where they play key ecological roles. In the present study, we identified three Tylenchidae species, namely Basiria bhabi, Coslenchus acceptus, and Filenchus vulgaris, using integrative taxonomy. The detailed morphological and morphometric characteristics, distribution, and host associations of each species were also discussed. Phylogenetic analyses of these populations with other Tylenchidae nematodes indicated the presence of divergent lineages in Filenchus and Basiria, whereas Coslenchus appeared to be a monophyletic genus. Herein, we aim to grow awareness about this common but least studied group of nematodes. The species reported in this study are new records for Canada, revealing that the identified nematode diversity in our cultivated areas is relatively underrepresented. Our analyses also provided greater taxonomic resolution and captured rare taxa that might have been missed or misidentified in prior nematode inventory surveys. These findings will add to our understanding of the nematofauna of southern Alberta, thereby providing a more complete picture of existing nematode diversity present in the fields of this highly cultivated region.

Morphological and Molecular Characterization of Nothotylenchus medians and N. similis (Nematoda: Anguinidae) from Southern Alberta, Canada

“…Understanding the existing biodiversity of PPNs is important because of their wide range of survival adaptations and dispersal routes [24,25,27]. In our prior nematode inventory surveys, Nothotylenchus species were never detected because some field areas The results of both phylogenetic trees support the inclusion of Nothotylenchus species in the family Anguinidae and indicate that Nothotylenchus species are not monophyletic.…”

“…Understanding the existing biodiversity of PPNs is important because of their wide range of survival adaptations and dispersal routes [24,25,27]. In our prior nematode inventory surveys, Nothotylenchus species were never detected because some field areas (headland vegetation) had not been surveyed for nematode infestation.…”

“…Recently, several studies reported the presence of endo-and ectoparasitic nematodes from agricultural areas of southern Alberta, Canada [23][24][25][26][27]. Therefore, we performed detailed samplings in cultivated areas near Barnwell in southern Alberta, with the goal of characterizing the existing biodiversity of plant-parasitic nematodes (PPNs).…”

Molecular Characterization of Filenchus cylindricus (Thorne & Malek, 1968) Niblack & Bernard, 1985 (Tylenchida: Tylenchidae) from Korea, with Comments on Its Morphology

“…They do not represent economically important plant pathogens and are therefore grouped as weak plant parasites Siddiqi, 2000). In fact, their feeding behavior is still debatable, considering the fact that food preferences of most of the described species are unknown (Munawar et al, 2021). Some members are regarded as root hair feeders while several members of Filenchus Andrássy, 1954 can feed and reproduce on fungi (Bert et al, 2010;Okada et al, 2005;Siddiqi, 2000).…”

“…Some members are regarded as root hair feeders while several members of Filenchus Andrássy, 1954 can feed and reproduce on fungi (Bert et al, 2010;Okada et al, 2005;Siddiqi, 2000). Notwithstanding the mystery of their feeding behavior, members of this group play important ecological roles as soil health indicators (Bongers and Bongers, 1998;Munawar et al, 2021).…”