First Report of the Reniform Nematode (<i>Rotylenchulus reniformis</i>) from Soybean in Paraguay

Lopez-Nicora, Horacio D.; Pedrozo, L M; Ocampos, Cristhian Grabowski; Fuente, Aida Lorenza Orrego; Villalba, E Hahn; Ralston, Timothy I.; Niblack, T. L.

doi:10.1094/pdis-02-18-0240-pdn

Cited by 5 publications

(2 citation statements)

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“…Several plant-parasitic nematode species have been reported in Paraguay [16][17][18]. However, these latter studies were conducted based only on the perineal pattern morphology of the nematodes: they allowed the identification of the species M. incognita and M. javanica affecting lettuce (Lactuca sativa) in the Central Department [19] and peanuts (Arachis hypogaea) in Conolinias Mennonitas, Chaco, Paraguay [17], respectively.…”

Section: Introductionmentioning

confidence: 99%

Enzymatic and Molecular Identification of Meloidogyne Species in Tomato Orchards in Paraguay

et al. 2023

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Tomato is a major crop in Paraguay, where it provides a source of employment and income for households. Tomato production can be affected by root-knot nematodes, especially Meloidogyne spp. The unequivocal identification of Meloidogyne spp. in Paraguay has not been conducted yet. This study aims to identify Meloidogyne species in eight tomato production districts of this country by biochemical and molecular techniques. Females of Meloidogyne spp. were extracted from tomato roots and characterized using esterase isozyme phenotypes. In addition, DNA was extracted from nematode eggs, and species-specific SCARs (sequence-characterized amplified regions) were used to confirm the diagnosis. Nematodes were detected in 100% of studied samples (prevalence), of which M. incognita (Est: I2, Rm: 1.1;1.2) and M. javanica (Est: J3, Rm: 1.0, 1.20, 1.35) were present in 39.13% and 26.08% of samples, respectively. One population (8.69%) of Meloidogyne sp. presenting an atypical esterase profile (Rm: 1.0 and 1.3) was only detected in Julián Augusto Saldívar District. Mixed populations, mostly M. incognita and M. javanica, were observed in 26.08% of samples. The SCAR primers incK14F/incK14R amplified specific fragments for M. incognita (399 bp) and M. javanica (670 bp), confirming the enzymatic results. Here, we present the first study of root-knot nematode identification at the species level in Paraguay.

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Section: Introductionmentioning

confidence: 99%

Enzymatic and Molecular Identification of Meloidogyne Species in Tomato Orchards in Paraguay

et al. 2023

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“…Reniform nematodes are sedentary semi-endoparasites commonly found in subtropical and tropical regions (LOPEZ-NICORA et al, 2018). Reniform nematodes are characterised by the attachment of females with their egg mass to plant roots and absence of typical swellings observed for root-knot nematodes (SIKORA, 2021).…”

Section: Plant-parasitic Nematodes and Their Impacts On Soybean Cropsmentioning

confidence: 99%

Interaction between soil microbiome and nematode activity in soybean crop

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Rotylenchulus reniformis (reniform nematode)

2022

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First Report of the Reniform Nematode (Rotylenchulus reniformis) from Soybean in Paraguay

Cited by 5 publications

References 3 publications

Enzymatic and Molecular Identification of Meloidogyne Species in Tomato Orchards in Paraguay

Enzymatic and Molecular Identification of Meloidogyne Species in Tomato Orchards in Paraguay

Interaction between soil microbiome and nematode activity in soybean crop

Rotylenchulus reniformis (reniform nematode)

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