First report of the occurrence and whole-genome characterization of            &lt;i&gt;Edwardsiella tarda&lt;/i&gt; in the false killer whale (&lt;i&gt;Pseudorca            crassidens&lt;/i&gt;)

Lee, Kyunglee; Kim, Hye Kwon; Park, Sung-Kyun; Sohn, Hawsun; Cho, Yuna; Choi, Youngjoon; Jeong, Dae Gwin; Kim, Ji Hyung

doi:10.1292/jvms.17-0590

Cited by 11 publications

(5 citation statements)

References 36 publications

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“…To resist phagocyte-mediated killing, various essential strategies, such as enzyme production including CAT, POD, and SOD, have been implicated by E. tarda to neutralize reactive oxygen species (ROS), while TTSS and T6SS contribute to invasion and subversion of the host cells (46). In the present study, 129 T3SS genes were predicted; however, in a previously reported E. tarda KC-Pc-HB1, no T3SS/T6SS genes were detected (41), indicating that SC002 is a more virulent strain.…”

Section: Discussioncontrasting

confidence: 83%

“…Unlike the published Edwardsiella complete genomes having one or two plasmids, the SC002 genome contained four novel plasmids ( 34 , 37 – 39 ). Subsequently, BLAST analysis shows that the pEtSC002-1 was identical to the S. enterica plasmid (NZ_CP037959.1), while pEtSC002-2, pEtSC002-3, and pEtSC002-4 were identical to the E. tarda KC_PC_HB1 plasmid (NZ_CP023707.1) ( 40 , 41 ). The antibiotic resistance determinants were only observed on pEtSC002-1 where the resistance gene patterns ( sul1, sul2, cml, tetA, aph33 , and aph6 ) were similar to those of EIB202 plasmid resistome ( 33 ).…”

Section: Discussionmentioning

confidence: 99%

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Complete genome sequence analysis of Edwardsiella tarda SC002 from hatchlings of Siamese crocodile

Rehman

Dawar²,

Zeng³

et al. 2023

Front. Vet. Sci.

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Edwardsiella tarda is a Gram-negative, facultative anaerobic rod-shaped bacterium and the causative agent of the systemic disease “Edwardsiellosis”. It is commonly prevalent in aquatic organisms with subsequent economic loss and hence has attracted increasing attention from researchers. In this study, we investigated the complete genome sequence of a highly virulent isolate Edwardsiella tarda SC002 isolated from hatchlings of the Siamese crocodile. The genome of SC002 consisted of one circular chromosome of length 3,662,469 bp with a 57.29% G+C content and four novel plasmids. A total of 3,734 protein-coding genes, 12 genomic islands (GIs), 7 prophages, 48 interspersed repeat sequences, 248 tandem repeat sequences, a CRISPR component with a total length of 175 bp, and 171 ncRNAs (tRNA = 106, sRNA = 37, and rRNA = 28) were predicted. In addition, the coding genes of assembled genome were successfully annotated against eight general databases (NR = 3,618/3,734, COG = 2,947/3,734, KEGG = 3,485/3,734, SWISS-PROT = 2,787/3,734, GO = 2,648/3,734, Pfam = 2,648/3,734, CAZy = 130/3,734, and TCDB = 637/3,734) and four pathogenicity-related databases (ARDB = 11/3,734, CARD = 142/3,734, PHI = 538/3,734, and VFDB = 315/3,734). Pan-genome and comparative genome analyses of the complete sequenced genomes confirmed their evolutionary relationships. The present study confirmed that E. tarda SC002 is a potential pathogen bearing a bulk amount of antibiotic resistance, virulence, and pathogenic genes and its open pan-genome may enhance its host range in the future.

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Section: Discussioncontrasting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Complete genome sequence analysis of Edwardsiella tarda SC002 from hatchlings of Siamese crocodile

Rehman

Dawar²,

Zeng³

et al. 2023

Front. Vet. Sci.

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“…Although it is considered to include potential pathogens to humans and animals ( 52 , 66 ), in the present study, its high abundance was not related to any pathogenies in the two pups. Edwardsiella has been associated with diseased cetaceans ( 67 ) while pathogenic species, like Edwardsiella tarda , have been isolated from non-diseased animals ( 48 , 68 , 69 ). This bacterium could be a threat for the released pups, as its abundance seemed to increase toward the end of the rehabilitation period.…”

Section: Discussionmentioning

confidence: 99%

Fecal and skin microbiota of two rescued Mediterranean monk seal pups during rehabilitation

Dosi,

Meziti,

Tounta

et al. 2024

Microbiol Spectr

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The role of animal host-associated microbiomes is becoming more apparent and defined for wild animals, especially for the species under conservation strategies. This study investigated the succession of fecal and skin bacterial microbiota of two rescued female Mediterranean monk seal ( Monachus monachus ) pups for most of their rehabilitation period. Bacterial species richness and diversity were assessed by high-throughput sequencing of nine freshly collected fecal samples and four skin swabs per individual. Both the fecal and skin microbiota highly overlapped in their containing operational taxonomic units (OTUs) and abundance patterns. The fecal microbiota was separated in two distinct periods and was dominated by OTUs related to the Shigella , Streptococcus , Enterococcus , Lactobacillus, and Escherichia genera in the first period, while in the second period, the dominating genera were the Clostridium, Blautia, Fusobacterium, Edwardsiella, and Bacteroides . The skin microbiota was highly similar between the two individuals in each sampling and was dominated by Psychrobacter-, Elizabethkingia-, and Bergeyella -related OTUs. The provided antibiotic treatment, along with the provided probiotics and nutritional supplements, resulted in a major turnover of the bacterial microbiota with the potentially detrimental OTUs being eliminated toward the end of the rehabilitation period, prior to the release of the pups in the wild. IMPORTANCE This study showed that during the rehabilitation of two rescued Mediterranean monk seal pups ( Monachus monachus ), the skin and fecal bacterial communities showed similar succession patterns between the two individuals. This finding means that co-housed pups share their microbiomes, and this needs to be considered in cases of infection outbreaks and their treatment. The housing conditions, along with the feeding scheme and care protocols, including the admission of antibiotics as prophylaxis, probiotics, and essential food supplements, resulted in bacterial communities with no apparent pathogenic bacteria. This is the first contribution to the microbiome of the protected seal species of M. monachus and contributes to the animal’s conservation practices through its microbiome.

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“…This finding was consistent with the pan-susceptible phenotypic AST profile of our isolate. The E. tarda KC-Pc-HB1 reference strain was initially isolated from the blood of a false killer whale ( Pseudorca crassidens ), a tropical and warm temperate dolphin species, that had been caught along the South Sea (Republic of Korea) ( Lee et al., 2018 ).…”

Section: Diagnostic Assessmentmentioning

confidence: 99%

Case Report: Disseminated Edwardsiella tarda infection in an immunocompromised patient

An,

Chan,

Nguyen

et al. 2023

Front. Cell. Infect. Microbiol.

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Human infection caused by bacteria of the Edwardsiella genus is rare and most often presents with gastroenteritis that rarely requires antibiotics. Our case report describes a medically complex patient with chronic steroid use contributing to an immunocompromised state, who presented with fever and abdominal pain. The patient was later found to have Edwardsiella tarda (E. tarda) bacteremia and underwent paracentesis confirming E. tarda bacterial peritonitis requiring a prolonged antibiotic course. This case report aims to illustrate the presentation, diagnosis, and management of an uncommon infection that can have severe complications especially among immunocompromised patients.

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First report of the occurrence and whole-genome characterization of <i>Edwardsiella tarda</i> in the false killer whale (<i>Pseudorca crassidens</i>)

Cited by 11 publications

References 36 publications

Complete genome sequence analysis of Edwardsiella tarda SC002 from hatchlings of Siamese crocodile

Complete genome sequence analysis of Edwardsiella tarda SC002 from hatchlings of Siamese crocodile

Fecal and skin microbiota of two rescued Mediterranean monk seal pups during rehabilitation

Case Report: Disseminated Edwardsiella tarda infection in an immunocompromised patient

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