First report of the <i>qnrA</i> determinant in <i>Shigella sonnei</i> isolated from China

Qian, Huimin; Qin, Tingting; Liu, G Y; Li, J; Ma, Ping; Kong, Xiaoxiao; Gu, Bing; Bao, Changjun

doi:10.1017/s0950268817000917

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2024

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Diarrheagenic Shigella

2024

Molecular Medical Microbiology

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Diarrheagenic Shigella

2024

Molecular Medical Microbiology

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Prevalence of plasmid-mediated quinolone resistance genes and biofilm formation in different species of quinolone-resistant clinical Shigella isolates: a cross-sectional study

Al-Khafaji,

Almjalawi,

Ewadh

et al. 2024

Eur J Med Res

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Background The purpose of this study was to look into the presence of plasmid-mediated quinolone resistance (PMQR) genes and biofilm formation in several species of clinical Shigella isolates that were resistant to quinolones. Methods The stool samples of 150 patients (younger than 10 years) with diarrhea were collected in this cross-sectional study (November 2020 to December 2021). After cultivation of samples on Hektoen Enteric agar and xylose lysine deoxycholate agar, standard microbiology tests, VITEK 2 system, and polymerase chain reaction (PCR) were utilized to identify Shigella isolates. The broth microdilution method was used to determine antibiotic susceptibility. PMQR genes including qnrA , qnrB , qnrC , qnrD , qnrE , qnrS , qnrVC , qepA , oqxAB , aac(6′)-Ib-cr , and crpP and biofilm formation were investigated in quinolone-resistant isolates by PCR and microtiter plate method, respectively. An enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) technique was used to determine the clonal relatedness of quinolone-resistant isolates. Results A total of 95 Shigella isolates including S. sonnei (53, 55.8%), S. flexneri (39, 41.1%), and S. boydii (3, 3.2%) were identified. The highest resistance rates of the isolates were against ampicillin (92.6%, n = 88/95). Overall, 42 of 95 (44.2%) isolates were simultaneously resistant against two or more quinolones including 26 (61.9%) S. sonnei and 16 (38.1%) S. flexneri . All isolates were multidrug-resistant (resistance to more than 3 antibiotics). The occurrence of PMQR genes was as follows: qnrS (52.4%), qnrA and aac(6′)-Ib-cr (33.3%), and qnrB (19.0%). The prevalence in species was as follows: 61.5% and 37.5% ( qnrS ), 19.2% and 56.3% ( qnrA ), 38.5% and 25.0 ( aac(6′)-Ib-cr ), and 19.2% and 18.8% ( qnrB ) for S. sonnei and S . flexneri , respectively. The other PMQR genes were not detected. In total, 52.8% (28/53) of quinolone-susceptible and 64.3% (27/42) of quinolone-resistant isolates were biofilm producers. Biofilm formation was not significantly different between quinolone-resistant and quinolone-susceptible isolates ( P -value ...

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First report of the qnrA determinant in Shigella sonnei isolated from China

Cited by 2 publications

References 20 publications

Diarrheagenic Shigella

Diarrheagenic Shigella

Prevalence of plasmid-mediated quinolone resistance genes and biofilm formation in different species of quinolone-resistant clinical Shigella isolates: a cross-sectional study

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