First report of the fungus Diaporthe phaseolorum on sunflower in Russia

Spring camelina is a promising oilseed crop for the dry steppe zone of Northern Kazakhstan. However, the expansion of its sowing areas is constrained by the lack of scientific research, especially on the phytosanitary state. The article presents the results of the study of fungi of the Alternaria and Fusarium genus isolated from the affected organs of spring camelina plants and provides measures to control them in the field conditions of the region. The research aimed to identify common diseases and determine their development and distribution under different backgrounds when treated with fungicides. To identify fungal strains, the method of determination of the direct nucleotide sequence of the intergenic transcribed region was used, followed by the determination of nucleotide identity with the sequences deposited in the Gene Bank international database, as well as the construction of phylogenetic trees with nucleotide sequences. As a result of the analysis, phytopathogenic strains belonging to Alternaria alternata, Alternaria tenuissima and Fusarium acuminatum were identified. During field studies, the development and spread of diseases under different background conditions of fungicide treatment versus control were analyzed. Of the products used, the Pictor fungicide (active ingredients: Boscalid 200 g/l + dimoxystrobin 200 g/l) showed high efficiency, compared with the Extrasolbiofungicide (Bacillus subtilis, strain H-13). The results of the study can serve as a basis for the development of chemical and biological control methods aimed at specific pathogens.

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“…Additionally, phylogenetic trees were constructed with sequences deposited in the GeneBank international database (Gomzhina and Gannibal, 2021).…”

Section: Characteristics Of Cultural and Morphological Features The S...mentioning

confidence: 99%

Development and Spread of Diseases in Spring Camelina (Camelina sativa (L.) Grantz) when using Various Treatments

Utelbayev¹,

Abysheva²,

Bazarbayev³

et al. 2021

OnLine Journal of Biological Sciences

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“…This is report about Diaporthe phaseolorum (Cooke & Ellis) Sacc. on sunflower in Krasnodar region [1]. The objective of this study was to correctly reidentify isolates of Diaporthe sp.…”

Section: Introductionmentioning

confidence: 99%

Diaporthe species infecting sunflower in Russia

Гомжина

Gannibal

2020

BIO Web Conf.

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Diaporthe is an important group of plant pathogenic fungi revealed all over the world. Early classification and species identification of this genus was mostly based on combination of morphological characteristics, cultural features, and affiliation with a host plant. According to recent investigations, valid distinction between Diaporthe species should have combined molecular techniques, morphological and cultural observations, and mating type data. In Russia a comprehensive and extensive analysis of biodiversity and geographic distribution of Diaporthe species infecting sunflower has not been performed. There were seven Diaporthe sp. strains isolated from this plant maintained in the Laboratory of Mycology and Phytopathology of All-Russian Institute of Plant Protection. In previous study a strain from Krasnodar region, based on combination of molecular and morphological features was identified as Diaporthe phaseolorum. The aim of this study was to identify all other strains using primarily molecular phylogenetic approach and traditional morphological analysis. The strains were identified as Diaporthe gulyae, Diaporthe eres, and Diaporthe helianthi. Two species - D. gulyae and D. eres are found for the first time on sunflower in Russia. Detection of D. helianthi is the first report of this fungus in Russia as confirmed by molecular analysis.

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First Report of Diaporthe phaseolorum Causing Stem Canker of Hemp (Cannabis sativa)

et al. 2021

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Hemp is an annual herbaceous plant that is used for its fiber and oil in a variety of commercial and industrial products. In Florida, it is currently being explored as a new specialty crop. During a field trial from October to January 2019 in Wimauma, FL, a stem canker was observed on up to 60% of three-month-old plants of 'Eletta Campana', 'Carmagnola Selezionata', and 'Tygra'. Symptoms started on the main stems with light-to-dark brown lesions of different sizes and shapes. Over time, the lesions coalesced into large necrotic areas and bore pycnidia. Isolations were made from diseased stem tissues on General Isolation medium (Amiri et al. 2018) after surface disinfestation (Marin et al. 2020). The plates were placed in a growth chamber at 25°C under a 12/12 photoperiod. A fungus with white, floccose, aerial mycelium and pycnidia producing alpha and beta conidia was consistently isolated. Three single spore isolates were chosen for identification and pathogenicity tests. Pycnidia on PDA were globose to irregular and ranged from 170 to 250 μm long (210 ± 2.5, n = 50) and 140 to 220 μm wide (180 ± 2.7, n = 50). The alpha conidia were unicellular, hyaline, ellipsoidal to fusiform and ranged from 5.3 to 7.7 μm long (6.5 ± 1.6, n = 50) and 1.5 to 4.6 μm wide (2.8 ± 1.8, n = 50). The beta conidia were hyaline, elongated, filiform, straight or curved and ranged from 10.2 to 17.7 μm long (16.1 ± 2.2, n = 50) and 0.5 to 1.8 μm wide (0.8 ± 0.2, n = 50). Perithecia were not observed. Based on morphological features, the fungus was similar to anamorphs of Diaporthe spp. (Santos et al. 2011; Udayanga et al. 2015). DNA from the same three isolates was extracted using the FastDNA kit, and the ribosomal internal transcribed spacer (ITS), β-tubulin (TUB), and calmodulin (CAL) regions were amplified following Udayanga et al. (2014), and Sanger sequenced by Genewiz. Sequences were deposited in GenBank (accession no. MT497039 to MT497047 for ITS, TUB, and CAL). BLASTn searches revealed isolates 20-58, 20-59, and 20-60 were 96.34% identical to the epitype isolate D. phaseolorum AR4203 for ITS (KJ590738.1, 527 bp out of 547 bp), 100% for TUB (KJ610893.1, 459 bp out of 459 bp), and 100% for CAL (KJ612135.1, 522 bp out of 522 bp) (Udayanga et al. 2015). Their identity was confirmed by phylogenetic analyses using maximum likelihood and Bayesian inference methods. To complete Koch’s postulates, pycnidia of the same three isolates were harvested and crushed in 2 mL Eppendorf tubes containing 0.01% Tween 20. Conidia suspensions were adjusted to 106 spores/mL. Three 5-week-old potted plants of 'Eletta Campana' and 'Carmagnola Selezionata' per isolate were inoculated using a 1 mL syringe with a needle by injecting 200 µL of the suspension into the stem. Plants were placed inside clear plastic bags for 48 h and maintained in the greenhouse. Control plants were injected with sterile deionized water and kept under the same conditions. The pathogenicity test was repeated once. Four weeks after inoculation, inoculated plants developed stem cankers from which the same pathogen was isolated, whereas controls remained healthy. To our knowledge, this is the first report of D. phaseolorum causing stem canker on hemp. This pathogen has been reported causing canker on sunflower and Phaseolus spp. (Gomzhina and Gannibal 2018; Udayanga et al. 2015; Vrandecic et al. 2009). This discovery may help shape future research into disease epidemiology and management for a crop in which very limited disease information is available at the moment.

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First report of the fungus Diaporthe phaseolorum on sunflower in Russia

Cited by 4 publications

References 26 publications

Development and Spread of Diseases in Spring Camelina (Camelina sativa (L.) Grantz) when using Various Treatments

Development and Spread of Diseases in Spring Camelina (Camelina sativa (L.) Grantz) when using Various Treatments

Diaporthe species infecting sunflower in Russia

First Report of Diaporthe phaseolorum Causing Stem Canker of Hemp (Cannabis sativa)

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