First report of the complete mitogenome of Tanypus punctipennis Meigen, 1818 (Diptera, Chironomidae) from Hebei Province, China

Abstract

Tanypus punctipennis Meigen, 1818 is an important bioindicator for freshwater ecosystems monitoring. Although COI barcode analyses have been performed on T. punctipennis , the mitogenome of this taxon has not been assembled and analyzed. Here, the complete mitogenome of T. punctipennis was sequenced and analyzed to confirm the systematic and phylogenetic history of this species. The mitogenome is 16,215 bp long with high A + T content, and consists of 13 protein-coding genes, 22 tRNA genes, two rRNA genes, and a noncoding control region. The phylogenomic analysis supports monophyletic Tanypodinae and close relationship between T. punctipennis and Clinotanypus . Our results indicate that mitogenomes showed strong signals in phylogenetic reconstructions at the genus level of Tanypodinae.

First Report on Mitochondrial Gene Rearrangement in Non-Biting Midges, Revealing a Synapomorphy in Stenochironomus Kieffer (Diptera: Chironomidae)

“…Published mitogenome data of Chironomid are relatively rare compared with other insects of Diptera. Previous studies have focused on the genome organization and the application of nucleotide sequence information in phylogenetic analysis [30][31][32][33][34][35][36][37]. However, mitochondrial gene rearrangement has never been reported in the Dipteran family Chironomidae.…”

Mitogenomes provide new insights of evolutionary history of Boreheptagyiini and Diamesini (Diptera: Chironomidae: Diamesinae)

“…Benefiting from the advances of high‐throughput sequencing technology, an increasing number of complete mitogenomes have been sequenced among the Diptera (Kang et al, 2016 ; Li et al, 2020 ; Miao et al, 2020 ; Ramakodi et al, 2015 ; Tang, Yan, et al, 2019 ; Wang et al, 2021 ; Yan et al, 2021 ; Zhang et al, 2022 ), and have been widely used for mitochondrial structure comparison and phylogenetic analysis at different taxonomic levels (Chen et al, 2018 ; de Oliveira Aragão et al, 2019 ; Yan et al, 2019 ; Zhang et al, 2016 ; Zhang, Kang, et al, 2019 ). Prior to this study, rare mitogenomes of Chironomidae were available (Beckenbach, 2012 ; Deviatiiarov et al, 2017 ; Fang et al, 2022 ; Jiang et al, 2022 ; Kim et al, 2016 ; Kong et al, 2021 ; Lei et al, 2021 ; Park et al, 2020 ; Zhang, Xu, et al, 2019 ; Zheng et al, 2022 ; Zheng et al, 2021 ), limiting our understanding of their mitochondrial structure and phylogenetic pattern. Besides, it is still unknown whether mitogenomes can effectively resolve phylogenetic relationships at different levels within Chironomidae.…”