First Report of Sweet Potato Symptomless Virus 1 Infecting Ipomoea batatas in Brazil

Souza, C. A.; Rossato, Maurício; Melo, Fernando Lucas; Boiteux, L. S.; Pereira–Carvalho, R. C.

doi:10.1094/pdis-01-18-0083-pdn

Cited by 6 publications

(3 citation statements)

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“…SPSMV-1 was first reported in Peru in 2009 ( Cao et al, 2017 ; Kreuze et al, 2009 ; Muhire et al, 2013 ). Since then, the virus has been reported in several countries in the Americas, East Africa, Europe, and Northeast Asia including USA, Uruguay, Brazil, Kenya, Spain, Taiwan, and China ( Kwak et al, 2014 ; Mbanzibwa et al, 2014 ; Souza et al, 2018 ). SPSMV-1 was first identified in sweet potatoes in the Korean peninsula in 2012 ( Kwak et al, 2014 ).…”

mentioning

confidence: 99%

“…Although SPSMV-1 does not induce major symptoms in sweet potato plants, co-infection with different disease-inducing sweet potato viruses is highly prevalent, and threatens sweet potato production ( Souza et al, 2018 ). In this study, an isolated SPSMV-1 Korean infectious clone was constructed, and agro-inoculation assays with different Agrobacterium tumefaciens strains were performed on the model plant Nicotinana benthamiana to confirm the function of the infectious clone and, later, investigate the interaction between SPSMV-1 and its host plant.…”

mentioning

confidence: 99%

“…the Americas, East Africa, Europe, and Northeast Asia including USA, Uruguay, Brazil, Kenya, Spain, Taiwan, and China (Kwak et al, 2014;Mbanzibwa et al, 2014;Souza et al, 2018). SPSMV-1 was first identified in sweet potatoes in the Korean peninsula in 2012 (Kwak et al, 2014).…”

mentioning

confidence: 99%

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Construction of an Agroinfectious Clone of a Korean Isolate of Sweet Potato Symptomless Virus 1 and Comparison of Its Infectivity According to Agrobacterium tumefaciens Strains in Nicotiana benthamiana

Byun

Lee

et al. 2023

Plant Pathol J

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Sweet potato symptomless virus 1 (SPSMV-1) is a single-stranded circular DNA virus, belonging to the genus Mastrevirus (family Geminiviridae) that was first identified on sweet potato plants in South Korea in 2012. Although SPSMV-1 does not induce distinct symptoms in sweet potato plants, its co-infection with different sweet potato viruses is highly prevalent, and thus threatens sweet potato production in South Korea. In this study, the complete genome sequence of a Korean isolate of SPSMV-1 was obtained by Sanger sequencing of polymerase chain reaction (PCR) amplicons from sweet potato plants collected in the field (Suwon). An infectious clone of SPSMV-1 (1.1-mer) was constructed, cloned into the plant expression vector pCAMBIA1303, and agro-inoculated into Nicotiana benthamiana using three Agrobacterium tumefaciens strains (GV3101, LBA4404, and EHA105). Although no visual differences were observed between the mock and infected groups, SPSMV-1 accumulation was detected in the roots, stems, and newly produced leaves through PCR. The A. tumefaciens strain LBA4404 was the most effective at transferring the SPSMV-1 genome to N. benthamiana. We confirmed the viral replication in N. benthamiana samples through strand-specific amplification using virion-sense- and complementary-sense-specific primer sets.

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