First Report of Strawberry Bacterial Angular Leaf Spot Caused by <i>Xanthomonas fragariae</i> in Tianjin, China

Wang, J.; Wei, Hengling; Chang, Ruokui; Liu, H. Q.; Wang, Y. H.

doi:10.1094/pdis-02-17-0182-pdn

Cited by 12 publications

(3 citation statements)

References 3 publications

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“…In case of severe infection, ALS reduces the yield and destroys the fruit quality, making it unsuitable for the market ( Wang et al., 2018 ). In Tianjin, China, the incidence of ALS in strawberries was first reported in 2016 ( Wang et al., 2017 ). Since then, this disease has been observed in multiple provinces of China, including Taiwan, Liaoning, Shanghai, and Yunnan ( Wu et al., 2020 ; Feng et al., 2021 ; Song et al., 2021 ; Zhang et al., 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Whole-genome sequencing and comparative genome analysis of Xanthomonas fragariae YM2 causing angular leaf spot disease in strawberry

Qiu,

Wei,

Meng

et al. 2023

Front. Plant Sci.

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BackgroundAngular leaf spot disease caused by plant pathogenic bacterium Xanthomonas fragariae seriously threatens strawberry crop production globally.MethodsIn this study, we sequenced the whole genome of X. fragariae YM2, isolated from Yunnan Province, China. In addition, we performed a comparative genome analysis of X. fragariae YM2 with two existing strains of X. fragariae YL19 and SHQP01 isolated from Liaoning and Shanghai, respectively.ResultsThe results of Nanopore sequencing showed that X. fragariae YM2 comprises one single chromosome with a contig size of 4,263,697 bp, one plasmid contig size of 0.39 Mb, a GC content ratio of 62.27%, and 3,958 predicted coding genes. The genome of YM2 comprises gum, hrp, rpf, and xps gene clusters and lipopolysaccharide (LPS), which are typical virulence factors in Xanthomonas species. By performing a comparative genomic analysis between X. fragariae strains YM2, YL19, and SHQP01, we found that strain YM2 is similar to YL19 and SHQP01 regarding genome size and GC contents. However, there are minor differences in the composition of major virulence factors and homologous gene clusters. Furthermore, the results of collinearity analysis demonstrated that YM2 has lower similarity and longer evolutionary distance with YL19 and SHQP01, but YL19 is more closely related to SHQP01.ConclusionsThe availability of this high-quality genetic resource will serve as a basic tool for investigating the biology, molecular pathogenesis, and virulence of X. fragariae YM2. In addition, unraveling the potential vulnerabilities in its genetic makeup will aid in developing more effective disease suppression control measures.

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Section: Introductionmentioning

confidence: 99%

Whole-genome sequencing and comparative genome analysis of Xanthomonas fragariae YM2 causing angular leaf spot disease in strawberry

Qiu,

Wei,

Meng

et al. 2023

Front. Plant Sci.

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“…These microorganisms induce diseases in most of the plant organs such as leaves, stems, inflorescences, fruits, and roots, reducing crop yields during pre-and postharvest. Several species are becoming a problem in berries worldwide, some as the bacteria Xanthomonas fragariae in strawberry (Kastelein et al 2014;Kamangar et al 2017;Wang et al 2017), the oomycete Phytophthora cinnamomi inducing crown and root rot in V. corymbosum (Tamietti 2003;Larach et al 2009;Huarhua et al 2018), the fungi Fusarium oxysporum causing wilt disease in blackberry (Gordon et al 2016;Pastrana et al 2017) and blueberries (Liu et al 2014;Moya-Elizondo et al 2019), or several species of Lasiodiplodia in different berries, as Lasiodiplodia theobromae causing dieback in strawberry (Nam et al 2016) or inducing stem blight and dieback of blueberry plants (Wright and Harmon 2009;Scarlett et al 2019;Rodríguez-Gálvez et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Pathogenic Microorganisms Infecting Berries in Mexico

Garay‐Serrano¹

2021

IJAB

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Mexico is one of the major producers of berries worldwide and ranks third amongst the principal exporters of these fruits. However, the presence of diseases in crops of blueberry, blackberry, strawberry, and raspberry in production areas of the country, has a negative impact in production yields. In this work, we presented a revision of all pathogens reported for these berries during the last 64 years in Mexico. Data on the different groups of pathogens including bacteria, stramenopila, and fungi, as well as location and references, are listed for the several types of berries produced. The pathogen species names were actualized according to the current taxonomic status following the specialized nomenclatural websites. Perspectives for future research are discussed. © 2021 Friends Science Publishers

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“…Since 1970, ALS has been reported in many European countries and has spread globally [9,30]. Reports on the newest occurrence of ALS are from China and Iran [27,67]. A comparative genomics approach using genome sequences of different Xf strains has been published and indicate high homogeneity of the sequenced Xf strains, although some differences have been detected in plasmids [14,18,64].…”

Section: Introductionmentioning

confidence: 99%

mRNA extraction of Xanthomonas fragariae in strawberry and validation of reference genes for the RT-qPCR for study of bacterial gene expression

2019

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This is the first study which describes a unique procedure of isolating of high-quality, intact RNA from strawberry leaves of Xanthomonas fragariae, three most suitable reference genes, crucial for the normalization of RT-qPCR data for this pathogen and accurate expression analysis of target genes. In our study, various mathematic algorithms: NormFinder geNorm, Best-Keeper, the delta CT method, RefFinder were adopted for validation of most stable reference genes from nine candidate genes (ffh, glyA, gyrA, gyrB, proC, pykA, recA, rpoB, rpoD). The analyses allowing to select three most suitable pioneer reference genes, gyrB, ffh, and pykA, that we recommend for the normalization of RT-qPCR data and for the study of the expression of target genes in Xf. Moreover, their combination as references allowed for an accurate expression analysis and computation of the fold change of the flhF and iroN2 genes in Xf. These two genes are important for the success of the colonization of plant tissue and pathogenicity and sequences of primers designed to study these genes, are presented.

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First Report of Strawberry Bacterial Angular Leaf Spot Caused by Xanthomonas fragariae in Tianjin, China

Cited by 12 publications

References 3 publications

Whole-genome sequencing and comparative genome analysis of Xanthomonas fragariae YM2 causing angular leaf spot disease in strawberry

Whole-genome sequencing and comparative genome analysis of Xanthomonas fragariae YM2 causing angular leaf spot disease in strawberry

Pathogenic Microorganisms Infecting Berries in Mexico

mRNA extraction of Xanthomonas fragariae in strawberry and validation of reference genes for the RT-qPCR for study of bacterial gene expression

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