First Report of Spring Viremia of Carp Virus (SVCV) in Wild Common Carp in North America

Development and characterization of monoclonal antibodies to spring viraemia of carp virus

“…Spring viraemia of carp (SVC), an important disease affecting cyprinids, mainly common carp (Cyprinus carpio), originally occurred in European carp culture (Ahne et al, 2002), recently to USA (Goodwin, 2002;Dikkeboom et al, 2004;Warg et al, 2007), China (Liu et al, 2004) and Canada (Garver et al, 2007). The causative agent of SVC is a rhabdovirus, spring viraemia of carp virus (SVCV), which is presently classified as a member of the genus Vesiculovirus of the family Rhabdoviridae (Fauquet et al, 2005).…”

Assessment of commercial test kits for identification of spring viraemia of carp virus

“…However, isolate M2-78 from Subgroup Id was not detected by the MAb. To further investigate that finding, 2 more isolates from Subgroup Id, 880163 and N3-14, that were the nearest neighbours to isolate M2-78 on the same branch of the neighbour-joining distance tree (Dikkeboom et al 2004 , was detected by the ELISA at a dilution of 1:16 and by the in-house IFAT, but was not detected by the IFAT test kit. Isolate N3-14 was closer than isolate 880163 to isolate M2-78 on the neighbour-joining distance tree.…”

“…Viruses were selected from distinct branches of the neighbour-joining distance tree (Table 1) within a Genogroup or subgroup (Stone et al 2003, Dikkeboom et al 2004 and grown at 20°C in Epithelioma papulosum cyprini cells (Fijan et al 1983) or fathead minnow cells (ATCC CCL 42) (isolate WI02-131 only) as described previously (Hill et al 1975, Way 1991, and harvested when complete cytopathic effect (CPE) was observed. The viruses were titrated in 96-well cell cultivation plates and TCID 50 values were calculated according to Kärber (1931).…”

Development and Evaluation of a Blocking Enzyme-Linked Immunosorbent Assay and Virus Neutralization Assay To Detect Antibodies to Viral Hemorrhagic Septicemia Virus

“…Spring viremia of carp virus (SVCV) is a rhabdovirus that is closely related to VHSV and that also causes disease during the spring season. The lysates were obtained from an isolate circulating during an SVCV epizootic in wild common carp (C. carpio) in northwestern Wisconsin (40). VHSV and SVCV lysates were separated on 4 to 20% gradient gels by SDS-PAGE and transferred onto nitrocellulose membranes (Bio-Rad) using a wet transfer Mini Trans-Blot cassette according to the manufacturer's protocols in the Mini-PROTEAN Precast Gels Instruction Manual and Application Guide (BioRad), with the following modifications.…”