First Report of Species of Colletotrichum Causing Leaf Spot of Liriodendron chinense × tulipifera in China

Zhu, Li‐Hua; Wan, Yu; Zhu, Ya‐Nan; Liu, C.-L.; Li, De‐Wei

doi:10.1094/pdis-12-18-2265-pdn

Cited by 20 publications

(3 citation statements)

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“…Previous reports showed that anthracnose disease was caused by C. gloeosporioides and C. siamense on L. chinense × tulipifera in China (Zhu et al, 2019) and C. gloeosporioides on L. tulipifera in Korea (Okryun et al, 2012). Colletotrichum fructicola is widely distributed on a variety of host plants, such as shine muscat, apple and so on (Fu et al, 2013;Lim et al, 2020).…”

Section: Re Sults and Discussionmentioning

confidence: 99%

First report of Colletotrichum fructicola causing leaf spots on Liriodendron chinense × tulipifera in China

Wan

Jin

et al. 2022

Forest Pathology

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Liriodendron chinense × tulipifera is widely planted in China as an ornamental plant. In July 2018, a foliar disease, with an incidence of ~23% trees and ~ 45% leaves per individual tree infected, occurred on L. chinense × tulipifera on the campus of Nanjing Forestry University, Jiangsu Province, China. Symptoms were small brown to dark spots surrounded by a yellow halo initially, which enlarged and coalesced into regular or irregular brown necrotic lesions up to 8.5 ± 1.3 mm across. Fungi were isolated from the margins of the lesions. Three representative isolates (E3‐2, E3‐3 and E3‐4) were selected for phylogenetic analyses. One representative isolate E3‐2 was used for pathogenicity tests and morphological identification. The pathogenicity of isolate E3‐2 to L. chinense × tulipifera was confirmed on 1‐year‐old seedlings with lesions after 5 days. The phenotypic characteristics of the isolates were similar to those of Colletotrichum sp. (Glomerellaceae). Phylogenetic analyses using concatenated sequences of ITS, ACT, CHS, GADPH, CAL, SOD2 and TUB2 placed E3‐2, E3‐3 and E3‐4 in the clade of C. fructicola. Based on the morphological and phylogenetic studies, the isolates were identified as C. fructicola. This is the first report of C. fructicola causing L. chinense × tulipifera leaf spots in China.

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Section: Re Sults and Discussionmentioning

confidence: 99%

First report of Colletotrichum fructicola causing leaf spots on Liriodendron chinense × tulipifera in China

Wan

Jin

et al. 2022

Forest Pathology

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“…Small (5 × 5 mm) tissue samples were excised from the necrotic tissue margins, resulting in a total of 20 pieces. These tissues were surface sterilized using established procedures [39], subsequently placed onto potato dextrose agar (PDA) plates, and incubated at 25 • C in darkness for 3 days. Pure cultures were obtained by transferring mycelial edges onto fresh PDA plates.…”

Section: Sampling and Fungal Isolationmentioning

confidence: 99%

Novel Pathogen–Plant Host Interaction: Colletotrichum jiangxiense and Fraxinus americana L. (White Ash) in a Sentinel Garden in China

Chang,

Li,

Gao

et al. 2023

Plants

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Fraxinus americana L. (white ash), a native North American tree commonly cultivated for its ornamental qualities, displayed symptoms of leaf spot disease in a sentinel garden located in Nanjing, Jiangsu, China, in 2022. This disease led to premature leaf shedding, adversely affecting the plant’s growth and substantially diminishing its ornamental value. Potential fungal pathogens were isolated from the diseased leaves and the subsequent application of Koch’s postulates confirmed the pathogenicity of the fungal isolates (BL-1, BL-2). Through a combination of multi-locus phylogenetic analysis, including ITS, ACT, ApMat, CAL, CHS-1, GAPDH, and TUB2, alongside morphological assessments, the fungus was conclusively identified as Colletotrichum jiangxiense. This represents the first record of C. jiangxiense affecting white ash, highlighting the important role of sentinel gardens in uncovering novel pathogen–plant host interactions.

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“…Colletotrichum gloeosporioides is widely distributed in tropical and subtropical regions and can infect mango, peaches, Liriodendron chinense × tulipifera, Cunninghamia lanceolata, and Camellia sinensis, causing anthracnose in leaves and fruits (Huang et al, 2018;Tang et al, 2019;Wu et al, 2019;Zhu et al, 2019;Shang et al, 2020). The conidia of this pathogen can infect the tender leaves, twigs, flowers and fruits of the host, causing leaves, flowers and fruits to fall, and fruit to decay during storage, resulting in serious economic losses.…”

Section: Introductionmentioning

confidence: 99%

Antifungal Effects of Volatile Organic Compounds Produced by Rahnella aquatilis JZ-GX1 Against Colletotrichum gloeosporioides in Liriodendron chinense × tulipifera

Kong

Lin

et al. 2020

Front. Microbiol.

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The use of volatile organic compounds (VOCs) produced by microorganisms for the biological control of plant diseases has attracted much attention in recent years. In this study, the antifungal activity and identity of VOCs produced by Rahnella aquatilis JZ-GX1 isolated from the rhizosphere soil of pine were determined and analyzed. The effect of the VOCs on the mycelial growth of Colletotrichum gloeosporioides, the pathogen of Liriodendron chinense × tulipifera black spot, was determined by a joined-petri dish fumigation method. An in vitro leaf inoculation method was used to determine the fumigation effect of the VOCs on Liriodendron black spot. VOCs with antifungal activity were collected by headspace solid-phase microextraction (SPME), and their components were analyzed by gas chromatography-mass spectrometry (GC-MS). The results showed that the VOCs secreted by JZ-GX1 inhibited the mycelial growth of the tested pathogen. The VOCs destroyed the morphology of the mycelium, significantly increased the permeability of the cell membrane and downregulated the expression of pathogenicity-related genes during mycelial infection, thus inhibiting the expansion of anthracnose disease spots in leaves. In the volatile compound profile, 3-methyl-1-butanol and 2-phenylethyl methyl ether significantly inhibited the mycelial growth and spore germination of C. gloeosporioides. This work provides a new strategy for the research and application of microorganisms and bioactive compounds to control plant anthracnose.

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First Report of Species of Colletotrichum Causing Leaf Spot of Liriodendron chinense × tulipifera in China

Cited by 20 publications

References 3 publications

First report of Colletotrichum fructicola causing leaf spots on Liriodendron chinense × tulipifera in China

First report of Colletotrichum fructicola causing leaf spots on Liriodendron chinense × tulipifera in China

Novel Pathogen–Plant Host Interaction: Colletotrichum jiangxiense and Fraxinus americana L. (White Ash) in a Sentinel Garden in China

Antifungal Effects of Volatile Organic Compounds Produced by Rahnella aquatilis JZ-GX1 Against Colletotrichum gloeosporioides in Liriodendron chinense × tulipifera

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