First report of Sclerotinia trifoliorum stem and crown rot on Trifolium alexandrinum in Pakistan

Faraz, Anjum; Haq, Imran Ul; Ijaz, Siddra

doi:10.1007/s42161-021-00806-4

Cited by 5 publications

(2 citation statements)

References 2 publications

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“…Seed sowing of each cultivar was in trays filled with an equal ratio of clay and soil mixture and kept under a glasshouse. Inoculation of plants was performed using the sick soil method by mixing inoculum of Sclerotinia trifoliorum [9] (the fungal pathogen of stem and crown rot disease) in the soil.…”

Section: Plant Materials Collection and Inoculationmentioning

confidence: 99%

Identification, in silico characterization, and expression analysis of NBS-LRR class of R genes against stem and crown rot disease in Trifolium alexandrinum L.

Nasir¹,

Ijaz²,

Haq³

et al. 2023

ScienceAsia

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R genes with colossal contributions to disease resistance possess conserved domains with confounded roles. Based on the conserved domains, R genes encoded proteins are classified. The members of nucleotide-binding site, NBS-Leucine rich repeats, LRR class of R genes are significant candidates involved in plant disease-resistance pathways. In this study, the genetic elements contributing to stem and crown rot disease resistance were explored through transcriptome probing based on conserved NBS domain under disease stress in berseem clover. Expression analysis of identified gene sequences revealed their upregulation during stem and crown rot disease. The in silico characterization imparted strong support towards the predictive role of identified RGAs in disease resistance.

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Section: Plant Materials Collection and Inoculationmentioning

confidence: 99%

Identification, in silico characterization, and expression analysis of NBS-LRR class of R genes against stem and crown rot disease in Trifolium alexandrinum L.

Nasir¹,

Ijaz²,

Haq³

et al. 2023

ScienceAsia

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“…Trichoderma spp. a biocontrol agent directly competes for nutrients and space and indirectly behaves as mycoparasitic fungi against phytopathogens (Jeger et al, 2009;Faraz et al, 2022). Trichoderma is an important source to produce enzymes and is very beneficial in the recycling of waste material.…”

Section: Introductionmentioning

confidence: 99%

Morpho-Molecular Characterization of Trichoderma Isolates from Rhizospheric Soils of Vegetables in Pakistan

Iqbal¹,

Ashfaq²,

Malik³

et al. 2022

Int. J. Phytopathol.

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Trichoderma, a major fungal genus attaining importance due to its diverse application in biological control programs and is considered a substitute for chemical pesticides. This research was conducted to characterize various Trichoderma species isolated from rhizospheric soil samples morphologically followed by its confirmation using molecular tools. A systematic survey of Trichoderma populations associated with soils of different vegetable hosts would enable a clear picture of the distribution of species in the region. Samples were collected from the rhizospheres of a variety of vegetable hosts and obtained numerous Trichoderma isolates (T. harzianum, T. viride, T. hamatum, T. longibrachiatum, T. asperellum, T. koningii and T. longipile). Morphological characteristics revealed that T. harzianum resembles T. viride but is more pigmented with confined rings than T. viride and other associated species. T. viride sporulation was more rapid than other species, producing a soft mat on PDA media. T. viride produces a sweet smell of coconut; T. asperellum produces a misty odour while T. longibrachiatum produces a yellow pigmentation in the media. Fifty out of 200 morphologically identified species were genetically characterized using universal primers (ITS-1 and ITS-4). ITS-based sequencing resulted in a product of 650 bp in all the isolates. The sequencing of these isolates showed five different species. As per rDNA, the species identified are: T. harzianum, T. hamatum, T. longibrachiatum, T. asperellum and T. viride with 98-100% sequence similarities to other related Trichoderma isolates reported from China, India, Mexico, USA, Portugal, Germany, Spain and Brazil. Bioinformatics analysis was conducted using maximum parsimony (MP) that supports the resemblance of the present study Trichoderma species with species reported from other countries. It is concluded that Trichoderma strains with biocontrol activity are genetically different compared to the pathogenic ones. The findings of this study help in providing an opportunity to test these isolates against different plant pathogens and ultimately leads to the development of bio-pesticides that could be eco-friendly and cost-effective with no chance of resistance development

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Molecular Phytopathometry

Ijaz

Haq

Mukhtar

et al. 2022

Trends in Plant Disease Assessment

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First report of Sclerotinia trifoliorum stem and crown rot on Trifolium alexandrinum in Pakistan

Cited by 5 publications

References 2 publications

Identification, in silico characterization, and expression analysis of NBS-LRR class of R genes against stem and crown rot disease in Trifolium alexandrinum L.

Identification, in silico characterization, and expression analysis of NBS-LRR class of R genes against stem and crown rot disease in Trifolium alexandrinum L.

Morpho-Molecular Characterization of Trichoderma Isolates from Rhizospheric Soils of Vegetables in Pakistan

Molecular Phytopathometry

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