First report of Sarcocystis spp. (Apicomplexa, Sarcocystidae) in Lagostomus maximus (Desmarest, 1917) (Rodentia, Chinchillidae) in Argentina

Canova, Victoria; Helman, Elisa; Robles, María del Rosario; Abba, Agustín M.; Moré, Gastón

doi:10.1016/j.ijppaw.2023.03.001

Cited by 9 publications

(10 citation statements)

References 18 publications

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“…Interestingly, the two 18S clones from the boa sporocysts also had high identities with Sarcocystis sp. from L. maximus in Argentina ( Canova et al, 2023 ), coorroborating the results obtained with cox1 analysis.…”

Section: Discussionmentioning

confidence: 78%

“…Molecular analysis of the sporocysts shed by the boa was performed using PCR targeted to four genetic markers ( cox1 , 18S , ITS1 and 28S ). Sequencing of cox1 revealed a high quality consensus sequence, which had 100% identity with Sarcocystis found in the rodent L. maximus in Argentina ( Canova et al, 2023 ). On the other hand, sequencing of PCR amplicons derived from 18S and ITS1 yielded chromatograms with several double picks and ambiguous results, suggesting that the snake probably shed sporocysts of more than one Sarcocystis spp.…”

Section: Discussionmentioning

confidence: 99%

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Sarcocystis sp. shed by the common boa snake (Boa constrictor) in Brazil

Bezerra,

de Jesus,

Soares

et al. 2023

International Journal for Parasitology: Parasites and Wildlife

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Section: Discussionmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Sarcocystis sp. shed by the common boa snake (Boa constrictor) in Brazil

Bezerra,

de Jesus,

Soares

et al. 2023

International Journal for Parasitology: Parasites and Wildlife

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“…However, due to the low level of research on different rodent species, it is suggested that the true number of Sarcocystis spp. in these hosts is higher [ 12 , 13 ]. In recent years, several new Sarcocystis species have been described in rodents [ 14 , 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Detection of Three Sarcocystis Species (Apicomplexa) in Blood Samples of the Bank Vole and Yellow-Necked Mouse from Lithuania

Prakas,

Gudiškis,

Kitrytė

et al. 2024

Life

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The genus Sarcocystis is an abundant group of Apicomplexa parasites found in mammals, birds, and reptiles. These parasites are characterised by the formation of sarcocysts in the muscles of intermediate hosts and the development of sporocysts in the intestines of definitive hosts. The identification of Sarcocystis spp. is usually carried out in carcasses of animals, while there is a lack of studies on the detection of Sarcocystis species in blood samples. In the current study, blood samples of 214 yellow-necked mice (Apodemus flavicollis) and 143 bank voles (Clethrionomys glareolus) from Lithuania were examined for Sarcocystis. The molecular identification of Sarcocystis was carried out using nested PCR of cox1 and 28S rRNA and subsequent sequencing. Sarcocystis spp. were statistically (p < 0.01) more frequently detected in the bank vole (6.3%) than in yellow-necked mice (0.9%). The analysed parasites were observed in four different habitats, such as mature deciduous forest, bog, natural meadow, and arable land. Three species, Sarcocystis funereus, Sarcocystis myodes, and Sarcocystis cf. glareoli were confirmed in the bank vole, whereas only Sarcocystis myodes were found in yellow-necked mice. The obtained results are important in the development of molecular identification of Sarcocystis parasites in live animals.

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“…Sarcocystosis is the most prevalent worldwide protozoal infection in several animal species (Moré et al 2008; Dubey et al 2016). In Argentina, various studies were carried out to identify Sarcocystis cysts in potential IH like cattle (Moré et al 2011), marsh deer (Berra et (Gual et al 2017), huemul and pudu (Reissig et al 2020), and more recently in rodents (Chinchillidae) (Canova et al 2023).…”

Section: Introductionmentioning

confidence: 99%

Identification of Sarcocystis spp. in synanthropic (Muridae) and wild (Cricetidae) rodents from Argentina

Bentancourt Rossoli,

Moré,

Soto-Cabrera

et al. 2023

Parasitol Res

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The occurrence of Sarcocystis species was investigated in synanthropic (Muridae) and wild (Cricetidae) rodents from Argentina. Nine species were captured (n = 356). Sarcocysts were detected in muscles of 8.7% (31/356) and 3.7% (4/106) of the rodents by histopathology and direct microscopy, respectively. PCRs-sequencing targeting the 18S rRNA, cox1 and ITS1 regions were performed on samples with positive histopathology. Four different 18S rRNA sequences or sequence groups with high intra-group identities (99.6-100%) were detected in Mus musculus, Oxymycterus rufus, Akodon azarae and Necromys lasiurus. Eight sequences showed 99.5-99.7% identity with S. dispersa. Thirteen sequences showed low identity (95.3-96.4%) with other Sarcocystis spp. The obtained coxI sequences (n = 9) were almost identical among them and showed a high similarity with S. strixi (99.2-99.5%) and S. lutrae (99.1%), despite the 18S rRNA sequences from the same samples suggested the occurrence of at least two species. This suggests that coxI may not show high variability in Sarcocystis spp. that use rodents as intermediate hosts. Six ITS1 sequences were obtained, showing high identity but low coverage with several Sarcocystis spp. Multilocus sequence typing and BLAST analysis did not allow accurate species identi cation. Possible reasons are the detection of new species or the lack of molecular information from previously described ones. Phylogeny suggests that the detected Sarcocystis spp. may use raptor birds or snakes as de nitive hosts. This study represents the rst molecular identi cation of Sarcocystis spp. in naturally infected rodents of the Cricetidae and Muridae families in South America.

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First report of Sarcocystis spp. (Apicomplexa, Sarcocystidae) in Lagostomus maximus (Desmarest, 1917) (Rodentia, Chinchillidae) in Argentina

Cited by 9 publications

References 18 publications

Sarcocystis sp. shed by the common boa snake (Boa constrictor) in Brazil

Sarcocystis sp. shed by the common boa snake (Boa constrictor) in Brazil

Detection of Three Sarcocystis Species (Apicomplexa) in Blood Samples of the Bank Vole and Yellow-Necked Mouse from Lithuania

Identification of Sarcocystis spp. in synanthropic (Muridae) and wild (Cricetidae) rodents from Argentina

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