First Report of Root Rot Caused by the Fusarium oxysporum Species Complex on Codonopsis pilosula in China

Abstract

Codonopsis pilosula Franch., also known as Dangshen, is an important medicinal plant in China. It is widely cultivated for a major income of local farmers in Dingxi, Gansu Province. Its dried roots have the effects of supplementing vital energy, nourishing spleen and lung, enhancing organic immunity, helping depressurization, and improving microcirculation, etc., for humans. In June to October, 2018-2020, root rot disease was observed on C. pilosula with incidences up to 20% in the Dingxi region. We collected ten diseased and healthy plants from Dingxi (35°06′N, 104°29′E, 2206 m a.s.l.) in October 2019. The rotting root tissues were sterilized with 70% ethanol for 30 s and 3% NaOCl for 5 min and placed on potato dextrose agar (PDA) plates incubated at 25℃to isolate the pathogen (Shang et al. 2014). From the similar fungal cultures isolated after 7 days on PGA, isolate B17 was purified for morphological and molecular characterization. Its colony appeared light purple and produced long aerial hyphae. Slightly curved macroconidia (12.3 to 31.7 × 3.1 to 5.1 μm, n=40) and oval-ellipsoid and cylindrical microconidia (6.1 to 9.9 × 2.8 to 4.5 μm, n=30) were observed. The internal transcribed spacer region (ITS) and the translation elongation factor-1 alpha (TEF-1α) gene were amplified using primers ITS1/ITS4 and EF-1/EF-2 (Uwaremwe et al. 2020), respectively. The 489 bp (ITS) and 631 bp (TEF-1α) sequences were deposited in GenBank (Accession No. MN744360 and MN786974, respectively). The ITS sequence had 100% homology to isolate JJF2 (No. MN626452, ITS) (Ma et al. 2020), and the TEF-1α sequence had 100% homology to isolate Fo353 (No. KM065860) (Koyyappurath et al. 2016) of Fusarium oxysporum Schlecht. emend. Snyder & Hansen, which caused root rot of Panax ginseng and Vanilla planifolia, respectively. A phylogenetic tree was generated using the unweighted pair-group method with arithmetic average in the MycoBank database (O’Donnell et al. 2015), which clustered isolate B17 in the F. oxysporum species complex. Twenty 1-year-old plants of C. pilosula were inoculated with were inoculated by dipping the washed roots in a conidial suspension (2 ×106 conidia/ml added with 0.2% Tween 20) for 20 min before transplanted into pots (16 × 16 × 23 cm) with four plants per pot filled with sterilized peat and soil mixture (2:1 v/v) and grown in a greenhouse at 26oC with >70% humidity and 16 h light. Sterilized water added with 0.2% Tween 20 was used as a control. One week after inoculation, the leaves of pathogen-inoculated plants became yellow, and wilting occurred at the leaf tips 18 days later. Some of the inoculated plants died 45 days after inoculation, and the low part of roots had dark brown to black lesions and became rotting. The control plants did not show symptoms. The pathogenicity test was repeated three times with the same fungus isolated from the infected root tissue. To the best of our knowledge, this is the first report that F. oxysporum causes root rot on C. pilosula in China. F. oxysporum is a serious threat to C. pilosula cultivation, and the finding of this pathogen provides a clear target for root rot control.

The Effect and Mechanism of New Processing Method of Codonopsis pilosula on Endocrine Physique Index in Rats

“…Codonopsis alkyne (lobetyolin) is one of the characteristic components of CP, which has a variety of physiological activities, such as anti-inflammatory, anticancer, and immunomodulatory activities [ 14 ]. Codonopsis alkynes are also one of the active components of CP protecting gastric mucosa [ 15 ]. In addition, CP also includes various sterols and alkaloids.…”

BushenHuoxue Recipe for the Treatment of Prethrombotic State of ACA-Positive Recurrent Miscarriage via the Regulation of the PI3K-AKT Signaling Pathway

“…Previous studies have shown that Salvia miltiorrhiza , Angelica sinensis , and Ligusticum chuanxiong improve blood circulation [ 30 – 32 ]. In addition, Salvia miltiorrhiza and Radix Angelicae sinensis prevent thrombosis [ 33 , 34 ], whereas Salvia miltiorrhiza and Codonopsispilosula improve the microcirculation [ 30 , 35 , 36 ] and regulate immunity [ 37 – 39 ]. In addition, the tonic recipe also intervenes in the metabolic pathways of the interconversion of pentose and glucuronide, lysine degradation, and histidine metabolism in the body.…”

Effects of Ozone Treatment on Postharvest Mucor Rot of Codonopsis pilosula Caused by Actinomucor elegans

“…A. elegans is widely distributed in soil, feces, grass, and air [7] and thrives under conditions of high temperature, high humidity, and poor ventilation, with the optimal growth and enzyme production environment being at temperatures of 25-30 • C, pH 6-7, and approximately 97% humidity. Similar to other fungi (like Penicillium and Monascus), A. elegans possesses strong decomposition capabilities for protein and fat [8,9]. Currently, the control of post-harvest diseases in fresh C. pilosula primarily relies on chemical agents such as sulfur dioxide fumigation.…”