First report of QoI resistance in Italian population of Pyricularia oryzae

Tenni, Daniele; Sinetti, Alice; Waldner, Maya; Torriani, Stefano F. F.; Romani, Marco

doi:10.1007/s41348-021-00494-3

Cited by 8 publications

(4 citation statements)

References 7 publications

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“…Although resistance to MBI‐D, isoprothiolane, carbendazim, iprobenfos and prochloraz has been extensively reported, 17,21–23,44 resistance of M. oryzae , causing rice blast, to QoI fungicides was only detected in Brazil and Italy 20,45 . Moreover, QoIs have been reported to stimulate pathogenicity‐associated toxins in some pathogens 46 . Because of this, a combination of QoIs and other fungicides with different modes of action was recommended in the practice.…”

Section: Discussionmentioning

confidence: 99%

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The G143A/S substitution of mitochondrially encoded cytochrome b (Cytb) in Magnaporthe oryzae confers resistance to quinone outside inhibitors

Gao

et al. 2022

Pest Management Science

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BACKGROUND: Rice blast, caused by Magnaporthe oryzae, is a destructive disease threatening the production of staple foods worldwide. Quinone outside inhibitors (QoIs) are a group of chemicals exhibiting excellent activity against a majority of plant pathogens, with the disadvantage that pathogens can easily develop resistance to QoIs.RESULTS: Here, we investigated the activity of picoxystrobin against M. oryzae, which showed a great inhibitory effect on 100 strains of M. oryzae with half-maximal effective concentrations (EC 50 ) ranging from 0.0251 to 0.1337 ∼g ml −1 . The EC 50 values showed a continuous unimodal distribution that was identical to the normal distribution, suggesting the potency of our study to represent baseline sensitivity. In addition, nine resistant mutants were obtained by exposing M. oryzae to a high dosage of picoxystrobin in the laboratory; all of them showed cross-resistance to the other five QoI fungicides. Although some mutants showed a decreased resistance factor after ten successive cultures on fungicide-free medium, the resistance to picoxystrobin was still inheritable. Amino acid substitution of G143S was detected in eight of nine picoxystrobin-resistant mutants, and G143A was detected in only one of nine mutants. A fitness penalty was found in the mutants carrying G143S rather than G143A.CONCLUSION: Our findings suggested that M. oryzae had a mid to high risk of resistance to picoxystrobin. Considering this, we should be vigilant to the resistance risk and apply picoxystrobin sensibly in the field.

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Section: Discussionmentioning

confidence: 99%

“…20,45 Moreover, QoIs have been reported to stimulate pathogenicity-associated toxins in some pathogens. 46 Because of this, a combination of QoIs and other fungicides with different modes of action was recommended in the practice.…”

Section: Discussionmentioning

confidence: 99%

The G143A/S substitution of mitochondrially encoded cytochrome b (Cytb) in Magnaporthe oryzae confers resistance to quinone outside inhibitors

Gao

et al. 2022

Pest Management Science

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“…Among these, strobilurin resistance in P. oryzae was first observed in strains isolated from perennial ryegrass, Lolium perenne [18], followed by its diffused presence in wheat isolates (Triticum aestivum) [2,19]. In rice isolates, QoI-resistance was first described in 2013 in Japan [20] and was absent from Europe until 2021, when it was detected in Italy [21]. This poses a threat to the European risiculture sector, as widespread distribution of such resistant strains would seriously compromise rice blast management with the available means.…”

Section: Of 18mentioning

confidence: 99%

Exploration of Novel Scaffolds Targeting Cytochrome b of Pyricularia oryzae

Pinna

Laurenzi

Forlani

et al. 2023

IJMS

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The fulfilment of the European “Farm to Fork” strategy requires a drastic reduction in the use of “at risk” synthetic pesticides; this exposes vulnerable agricultural sectors—among which is the European risiculture—to the lack of efficient means for the management of devastating diseases, thus endangering food security. Therefore, novel scaffolds need to be identified for the synthesis of new and more environmentally friendly fungicides. In the present work, we employed our previously developed 3D model of P. oryzae cytochrome bc1 (cyt bc1) complex to perform a high-throughput virtual screening of two commercially available compound libraries. Three chemotypes were selected, from which a small collection of differently substituted analogues was designed and synthesized. The compounds were tested as inhibitors of the cyt bc1 enzyme function and the mycelium growth of both strobilurin-sensitive (WT) and -resistant (RES) P. oryzae strains. This pipeline has permitted the identification of thirteen compounds active against the RES cyt bc1 and five compounds that inhibited the WT cyt bc1 function while inhibiting the fungal mycelia only minimally. Serendipitously, among the studied compounds we identified a new chemotype that is able to efficiently inhibit the mycelium growth of WT and RES strains by ca. 60%, without inhibiting the cyt bc1 enzymatic function, suggesting a different mechanism of action.

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“…In this light, the conventional disease control systems had become inefficient and essentially untrustworthy due to the ongoing agricultural cultivation's tendency to provoke disease outbreaks. Meanwhile, the fungicide resistance limits the fungicidal usage against plant pathogens, and it is evident with the emergence of Quinone outside Inhibitor (QoI) in the Italian population of Pyricularia oryzae has alarmed the farming community to revise and rethink about its practical credibility in the field (Magar et al, 2015; Tenni et al, 2021). Inappropriate and non‐judicious use of synthetic pesticides results in harmful effects to environment, which is of severe public concern today (Wang, Ma, et al, 2015; Wang, Mavrodi, et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Unveiling the potential of rice rhizospheric microbes and indigenous organic sources for the management of rice blast

Rajeswari

M²,

Sagar

et al. 2023

Journal of Phytopathology

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The rice blast incited by Pyricularia oryzae caused immense losses and left rice production in a predicament situation globally. Blast can be effectively controlled with synthetic fungicides. However, their indiscriminate use causes resistance among the pathogens coupled with environmental pollution issues. Exploitation of microbebased alternatives for the management of plant diseases diminishes public concerns

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First report of QoI resistance in Italian population of Pyricularia oryzae

Cited by 8 publications

References 7 publications

The G143A/S substitution of mitochondrially encoded cytochrome b (Cytb) in Magnaporthe oryzae confers resistance to quinone outside inhibitors

The G143A/S substitution of mitochondrially encoded cytochrome b (Cytb) in Magnaporthe oryzae confers resistance to quinone outside inhibitors

Exploration of Novel Scaffolds Targeting Cytochrome b of Pyricularia oryzae

Unveiling the potential of rice rhizospheric microbes and indigenous organic sources for the management of rice blast

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