Poa pratensis, an important cool-season perennial grass, is widely cultivated for construction of grasslands and ecological management of Qinghai-Tibet Plateau (Dong et al. 2020). Poa pratensis var. anceps Gaud cv. Qinghai (PPAQ) is a variant of P. pratensis (Liu et al. 2009). In June 2016, powdery mildew was observed on PPAQ in an artificial field of PPAQ in Haiyan county of Haibei Tibetan Autonomous Prefecture, Qinghai province, China (36°59′17.76″N, 100°52′54.01″E). Approximately 30 to 50% of leaves (approximate 10 ha) were affected. Initially, irregular white mycelial colonies were observed on the adaxial surface of affected leaves. The colonies increased in number and size, and later covered a large area of leaves and stems. In advanced stages of disease, the colonies covered the whole adaxial surface and white patches appeared on the abaxial surface of affected leaves, and eventually caused leaf death. Conidiophores were unbranched, measuring 160 to 235 × 4 to 13 μm, and borne vertically on hyphae. Each conidiophore produced 4 to 11 conidia in a chain. The conidia were oval, one-celled, and hyaline, measuring 22 to 40 ×10 to 21 μm (n = 50). Chasmothecia were yellow, spherical, and 172 to 240 μm in diameter (n = 20), each of which contained 8 to 17 asci. The appendages were few, and hyphoid. Asci were oblong or ovate, measuring 79 to 115 × 31 to 45 μm (n = 20). Asci were petiolate, containing eight ascospores. Ascospores were round to oval, colorless, one-celled, measuring 17 to 33 × 8 to 14 μm (n = 50). Based on morphological characteristics, the fungal organism was identified as Blumeria graminis (DC.) Speer. To confirm the identification, the internal transcribed spacer (ITS) of 612 bp was amplified from DNA of conidia using ITS5 and P3 primers (Takamatsu et al. 2009). The ITS sequence was deposited in GenBank database (Accession No. MF429949). The ITS showed 95% sequence similarity with those of B. graminis on Poa nemoralis in USA (Accession No. AB273560) and on P. bullbosa in Iran (Accession No. AB273551) (Inuma et al. 2007). Five two-month-old healthy plants were inoculated by spraying a spore suspension (1× 105 conidia ml-1) prepared from conidia brushed from infected plants; five plants sprayed with sterile distilled water served as controls. All the plants were maintained in a growth chamber with a constant temperature of 20°C, a 12 h/12 h light/dark diurnal cycle, and 70% humidity. Two weeks after inoculation, symptoms of powdery mildew were observed on all inoculated plants, whereas the control plants remained symptomless. The same fungus was confirmed by morphological characterization and molecular assays as described above. B. graminis has been reported on P. pratensis in USA (Dugan and Newcombe 2007), Israel (Voytyuk et al. 2009), and China (Zhang et al. 2014), but has not previously been reported on PPAQ. As far as we know, this is the first report of powdery mildew caused by B. graminis on PPAQ in China. These findings indicated that the health of PPAQ was substantially threaten when infected by powdery mildew, therefore, our results also contributed some valuable information how to diagnose this disease on PPAQ in China.
