First Report of Potential Biological Control of Potato Spindle Tuber Viroid Disease by Virus-Satellite Combination

Montasser, Magdy S.

doi:10.1094/pd-75-0319e

Cited by 10 publications

(6 citation statements)

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“…As shown in this study, the HV independence of Q-satRNA in multimer formation and nuclear localization suggests a possible evolutionary relationship to PSTVd, belonging to the family Pospiviroidae, which replicates in the nucleus by DNA-dependent RNA polymerase II via a rolling-circle mechanism (17). Interestingly, it was reported that the replication of PSTVd was severely inhibited when plants were coinfected with CMV and satRNA, whereas CMV alone had no effect on the replication of PSTVd (36,55). This may indicate that PSTVd and CMV satRNA are competing for the same host enzyme during replication in the nucleus or for the same host protein involved in the nuclear RNA import (i.e., VIRP 1).…”

Section: Discussionsupporting

confidence: 57%

Helper Virus-Independent Transcription and Multimerization of a Satellite RNA Associated with Cucumber Mosaic Virus

Choi

Seo

Kwon

et al. 2012

J Virol

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Satellite RNAs are the smallest infectious agents whose replication is thought to be completely dependent on their helper virus (HV). Here we report that, when expressed autonomously in the absence of HV, a variant of satellite RNA (satRNA) associated with Cucumber mosaic virus strain Q (Q-satRNA) has a propensity to localize in the nucleus and be transcribed, generating genomic and antigenomic multimeric forms. The involvement of the nuclear phase of Q-satRNA was further confirmed by confocal microscopy employing in vivo RNA-tagging and double-stranded-RNA-labeling assays. Sequence analyses revealed that the Q-satRNA multimers formed in the absence of HV, compared to when HV is present, are distinguished by the addition of a template-independent heptanucleotide motif at the monomer junctions within the multimers. Collectively, the involvement of a nuclear phase in the replication cycle of Q-satRNA not only provides a valid explanation for its persistent survival in the absence of HV but also suggests a possible evolutionary relationship to viroids that replicate in the nucleus.

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Section: Discussionsupporting

confidence: 57%

Helper Virus-Independent Transcription and Multimerization of a Satellite RNA Associated with Cucumber Mosaic Virus

Choi

Seo

Kwon

et al. 2012

J Virol

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“…It was previously observed that the replication of PSTVd was severely inhibited when the plants were coinfected with Cucumber mosaic virus and Q-satRNA, whereas Cucumber mosaic virus alone had no effect on the replication of PSTVd (29,30). Since PSTVd replicates in the nucleus and BRP1 plays a major role in PSTVd infection (22), we hypothesize that the inhibition of PSTVd replication in plants coinfected with Cucumber mosaic virus and Q-satRNA is due to competition for BRP1 by PSTVd and Q-satRNA.…”

Section: Resultsmentioning

confidence: 99%

“…Since Q-satRNA, like PSTVd, lacks an NLS, we envisioned that analogous to the case with PSTVd, nuclear importation of Q-satRNA could be mediated through its interaction with BRP1. Furthermore, it was observed that the replication of PSTVd was severely inhibited when the plants were coinfected with Cucumber mosaic virus and Q-satRNA, whereas Cucumber mosaic virus alone had no effect on the replication of PSTVd (29,30). Since both PSTVd and Q-satRNA have a nuclear phase in their replication cycles, it is reasonable to speculate that replication of PSTVd in coinfected plants was abated by inhibiting PSTVd entry to the nucleus.…”

Section: Discussionmentioning

confidence: 99%

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A Bromodomain-Containing Host Protein Mediates the Nuclear Importation of a Satellite RNA of Cucumber Mosaic Virus

Chaturvedi

Kalantidis

Rao

2014

J Virol

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However, we recently demonstrated that like with Potato spindle tuber viroid (PSTVd), a satRNA associated with Cucumber mosaic virus strain Q (Q-satRNA) has the propensity to localize in the nucleus and generate multimers that subsequently serve as templates for HV-dependent replication. But the mechanism regulating the nuclear importation of Q-satRNA is unknown. Here we show that the nuclear importation of Q-satRNA is mediated by a bromodomain-containing host protein (BRP1), which is also apparently involved in the nuclear localization of PSTVd. A comparative analysis of nuclear and cytoplasmic fractions from Nicotiana benthamiana plants coinfected with Q-satRNA and its HV confirmed the association of Q-satRNA but not HV with the nuclear compartment. A combination of the MS2-capsid protein-based RNA tagging assay and confocal microscopy demonstrated that the nuclear localization of Q-satRNA was completely blocked in transgenic lines of Nicotiana benthamiana (ph5.2nb) that are defective in BRP1 expression. This defect, however, was restored when the ph5.2nb lines of N. benthamiana were trans-complemented by ectopically expressed BRP1. The binding specificity of BRP1 with Q-satRNA was confirmed in vivo and in vitro by coimmunoprecipitation and electrophoretic mobility shift assays, respectively. Finally, infectivity assays involving coexpression of Q-satRNA and its HV in wild-type and ph5.2nb lines of N. benthamiana accentuated a biological role for BRP1 in the Q-satRNA infection cycle. The significance of these results in relation to a possible evolutionary relationship to viroids is discussed.

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“…The subcellular site of PSTVd replication is the nucleus and a bromodomain containing host protein (referred to as VRP1/BRP1) has been shown to promote nuclear import of PSTVd [48]. It is interesting to note that the replication of PSTVd was severely inhibited when the plants were co-infected with CMV and its sat-RNA, whereas CMV alone had no effect on the replication of PSTVd [94,95]. Since both PSTVd and sat-RNA have a nuclear phase in their replication cycles [93], it is reasonable to speculate that replication of PSTVd in co-infected plants was abated by inhibiting PSTVd entry to the nucleus.…”

Section: -General Aspectsmentioning

confidence: 99%

Infectious long non-coding RNAs

et al. 2015

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First Report of Potential Biological Control of Potato Spindle Tuber Viroid Disease by Virus-Satellite Combination

Cited by 10 publications

References 0 publications

Helper Virus-Independent Transcription and Multimerization of a Satellite RNA Associated with Cucumber Mosaic Virus

Helper Virus-Independent Transcription and Multimerization of a Satellite RNA Associated with Cucumber Mosaic Virus

A Bromodomain-Containing Host Protein Mediates the Nuclear Importation of a Satellite RNA of Cucumber Mosaic Virus

Infectious long non-coding RNAs

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