First report of Pepper mild mottle virus in pepper seeds produced in the Czech Republic - Short Communication

Abstract

Symptoms of viral infection were observed on plants of pepper, cv. OL 228, raised from commercial seeds of Czech origin in a greenhouse in the year 2002. Infected plants showed mosaic or mottling on leaves, and necrotic depressions on fruits. Straight, rod-shaped viral particles of about 300 nm, indicating a tobamovirus infection, were found by electron microscope. ELISA produced negative reactions for Tobacco mosaic virus (TMV) but positive reactions with an antiserum to Pepper mild mottle virus (PMMoV). In biological characterisation using pepper cultivars with the L1, L2, L3 and L4 tobamovirus resistance genes it was found that the Czech isolate of PMMoV belongs to pathotype P1.2. This is the first report of PMMoV in the Czech Republic. Its distribution, however, may still be limited as a survey did not reveal other infections in the main pepper producing areas. As PMMoV spreads with infected seeds, the possibility of its chemical deactivation by NaOH was tested and confirmed.

Seed transmissibility of viruses in winter squash landraces collected from the Black Sea region of Turkey

“…The sap was prepared by grinding CMV-and ZYMVinfected seedlings in a mortar and pestle with 0.1M phosphate buffer (pH = 7.2) (Usher et al 2012). The isolates were mechanically inoculated onto test plants of the following species: winter squash (C. maxima), pumpkin (C. moschata), cucumber (Cucumis sativus), and watermelon (Citrillus vulgaris), maintained in the controlled growth cabin at 24°C and monitored daily for symptom expression (Svoboda et al 2006). The winter squash seedlings for pathogenicity testing were obtained from seeds and were mechanically inoculated as mentioned previously for all tested plants.…”

Pepper mild mottle virus: a formidable foe of capsicum production—a review

“…PMMoV was reported as a distinct virus for the first time in Sicily, Italy (11). Since then, the virus has been reported in different countries where capsicum is widely cultivated, indicating its worldwide occurrence (11,(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31) (Figure 1). The virus is highly stable and remains infective even in irrigation/river/seawater, soil, compost, plant debris, etc.…”

Plant Growth-Promoting Rhizobacteria (PGPR) as Biocontrol Agents for Viral Protection

“…PMMoV, genus Tobamovirus, a well-known member of the family Virgaviridae, is an important pepper crop pathogen with a 100% infection on the field. This virus may easily spread mechanically either through seed coats infected with PMMoV or by grafting methods (Svoboda et al 2006). The activity of Pseudomonas oleovorans, strain KBPF-004, counter to pepper mild mottle virus was observed by Kim et al (2017) parallel to control/untreated strain, ATCC-8062.…”