First report of oxacillin-susceptible mecA-positive Staphylococcus aureus in healthy dogs and their owners in southern Brazil

Fabri, Franciele Viana; Pinto, Nattaly Bonacin; Mattos, Monica de Souza Ferreira de; Rodrigues, Regivaldo Florentino; Shinohara, Danielle Rosani; Pereira, Pedro Marquetti; Nishiyama, Sheila Alexandra Belini; Tognim, Maria Cristina Bronharo

doi:10.1016/j.prevetmed.2021.105286

“…In this study, an MRSP isolate from pet owners (18_W_M), which was positive for the mecA gene, did not show resistance against OXA. A similar finding was reported in the study conducted by Fabri et al [41]; that is, OXA-susceptible MRSA was isolated from nasal cavities of healthy dogs and their caretakers. While the reasons behind the susceptibility to OXA in MRSA remain unclear, some possible explanations Incorrect or overutilization of antimicrobials in different geographical regions plays a significant part in the development of resistance in bacteria.…”

Section: Discussionsupporting

confidence: 89%

Prevalence and multidrug-resistant profile of methicillin-resistant Staphylococcus aureus and methicillin-resistant Staphylococcus pseudintermedius in dogs, cats, and pet owners in Malaysia

Afshar

¹

,

Zakaria

²

,

Chen

³

et al. 2023

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Background and Aim: Staphylococcus aureus and Staphylococcus pseudintermedius are widespread skin and mucous membrane colonizers and may cause opportunistic infections in humans and animals. This study aimed to identify and characterize methicillin-resistant S. aureus (MRSA) and methicillin-resistant S. pseudintermedius (MRSP) isolates from domestic and stray dogs and cats and pet owners in Malaysia using molecular epidemiology and antimicrobial profiling. Materials and Methods: Three hundred and fifty oral and nasal swabs were taken from pet and stray dogs and cats and pet owners; all samples were subjected to culture and biochemical tests and polymerase chain reaction; the selected isolates were put through disk diffusion test and multilocus sequence typing. Results: One S. aureus isolate and three S. pseudintermedius isolates were identified as MRSA and MRSP, respectively, of which the MRSA isolate and one of the MRSP isolates showed multidrug resistance and the remaining two MRSP isolates were resistant to one or two antimicrobials. Multilocus sequence typing showed that the MRSA isolate belongs to clonal complex (CC) 789, while for the MRSP isolates, two were in CC45 and one was a singleton. Conclusion: This study is the first study in Malaysia to perform molecular characterization of MRSP isolated from pet dogs and cats and pet owners. The outcomes of this study reveal that even healthy pet dogs and cats and their owners can be carriers of drug-resistant staphylococci, highlighting the role of pets and pet owners as carriers of MRSA and MRSP in Malaysia. Keywords: antimicrobial resistance, antimicrobial susceptibility testing, methicillin resistance, multilocus sequence typing, public health, zoonotic infections.

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“…Whole bacteria transmission of ARB within households was especially demonstrated between dogs and their owners, despite a smaller proportion of studies in this review sampling pets (29%, 23/79). Up to 10% of dog-owner isolate pairs analyzed in rural Thailand, urban Iran, and Brazil were genetically related and had similar resistance phenotypes or ARG profiles. − In Rwanda, ESBL-producing E. coli ST38 was isolated from a hospitalized human and a related pet; ESBL-producing E.…”

Section: Resultsmentioning

confidence: 99%

Human Colonization with Antibiotic-Resistant Bacteria from Nonoccupational Exposure to Domesticated Animals in Low- and Middle-Income Countries: A Critical Review

Swarthout

¹

,

Chan

²

,

Garcia

³

et al. 2022

Environ. Sci. Technol.

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Data on community-acquired antibiotic-resistant bacterial infections are particularly sparse in low-and middleincome countries (LMICs). Limited surveillance and oversight of antibiotic use in food-producing animals, inadequate access to safe drinking water, and insufficient sanitation and hygiene infrastructure in LMICs could exacerbate the risk of zoonotic antibiotic resistance transmission. This critical review compiles evidence of zoonotic exchange of antibiotic-resistant bacteria (ARB) or antibiotic resistance genes (ARGs) within households and backyard farms in LMICs, as well as assesses transmission mechanisms, risk factors, and environmental transmission pathways. Overall, substantial evidence exists for exchange of antibiotic resistance between domesticated animals and in-contact humans. Whole bacteria transmission and horizontal gene transfer between humans and animals were demonstrated within and between households and backyard farms. Further, we identified water, soil, and animal food products as environmental transmission pathways for exchange of ARB and ARGs between animals and humans, although directionality of transmission is poorly understood. Herein we propose study designs, methods, and topical considerations for priority incorporation into future One Health research to inform effective interventions and policies to disrupt zoonotic antibiotic resistance exchange in low-income communities.

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“…A similar finding was reported in the study conducted by Fabri et al . [ 41 ]; that is, OXA-susceptible MRSA was isolated from nasal cavities of healthy dogs and their caretakers. While the reasons behind the susceptibility to OXA in MRSA remain unclear, some possible explanations include: (1) The presence of the transposable element IS1181 in the mec A gene, which leads to the suppression of PBP2a expression; (2) deletion of a single nucleotide from the area that codes for the N-terminal of PBP2a; (3) suppression of the mec A gene through the plasmid-encoded non-functional N-terminally truncated blaR1 sensor-transducer gene, even in the existence of beta-lactams; and (4) cell wall production is affected by mutations in the fem XAB genes, which encode proteins tangled in pentaglycine bridge production.…”

Section: Discussionmentioning

confidence: 99%

Prevalence and multidrug-resistant profile of methicillin-resistant Staphylococcus aureus and methicillin-resistant Staphylococcus pseudintermedius in dogs, cats, and pet owners in Malaysia

Afshar

¹

,

Zakaria

²

,

Chen

³

et al. 2023

Vet World

0

View full text Add to dashboard Cite

Background and Aim: Staphylococcus aureus and Staphylococcus pseudintermedius are widespread skin and mucous membrane colonizers and may cause opportunistic infections in humans and animals. This study aimed to identify and characterize methicillin-resistant S. aureus (MRSA) and methicillin-resistant S. pseudintermedius (MRSP) isolates from domestic and stray dogs and cats and pet owners in Malaysia using molecular epidemiology and antimicrobial profiling. Materials and Methods: Three hundred and fifty oral and nasal swabs were taken from pet and stray dogs and cats and pet owners; all samples were subjected to culture and biochemical tests and polymerase chain reaction; the selected isolates were put through disk diffusion test and multilocus sequence typing. Results: One S. aureus isolate and three S. pseudintermedius isolates were identified as MRSA and MRSP, respectively, of which the MRSA isolate and one of the MRSP isolates showed multidrug resistance and the remaining two MRSP isolates were resistant to one or two antimicrobials. Multilocus sequence typing showed that the MRSA isolate belongs to clonal complex (CC) 789, while for the MRSP isolates, two were in CC45 and one was a singleton. Conclusion: This study is the first study in Malaysia to perform molecular characterization of MRSP isolated from pet dogs and cats and pet owners. The outcomes of this study reveal that even healthy pet dogs and cats and their owners can be carriers of drug-resistant staphylococci, highlighting the role of pets and pet owners as carriers of MRSA and MRSP in Malaysia. Keywords: antimicrobial resistance, antimicrobial susceptibility testing, methicillin resistance, multilocus sequence typing, public health, zoonotic infections.

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First report of oxacillin-susceptible mecA-positive Staphylococcus aureus in healthy dogs and their owners in southern Brazil

Cited by 9 publications

References 20 publications

Prevalence and multidrug-resistant profile of methicillin-resistant Staphylococcus aureus and methicillin-resistant Staphylococcus pseudintermedius in dogs, cats, and pet owners in Malaysia

Prevalence and multidrug-resistant profile of methicillin-resistant Staphylococcus aureus and methicillin-resistant Staphylococcus pseudintermedius in dogs, cats, and pet owners in Malaysia

Human Colonization with Antibiotic-Resistant Bacteria from Nonoccupational Exposure to Domesticated Animals in Low- and Middle-Income Countries: A Critical Review

Prevalence and multidrug-resistant profile of methicillin-resistant Staphylococcus aureus and methicillin-resistant Staphylococcus pseudintermedius in dogs, cats, and pet owners in Malaysia

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