First report of NDM-1-producing clinical isolate of Leclercia adecarboxylata in Spain

Riazzo, Cristina; López-Cerero, Lorena; Rojo-Martín, María Dolores; Hoyos‐Mallecot, Yannick; Fernández-Cuenca, Felipe; Martín-Ruíz, Jose Luis; Pascual, Álvaro; Naas, Thierry; Navarro-Marí, José María

doi:10.1016/j.diagmicrobio.2017.04.013

Cited by 20 publications

(14 citation statements)

References 27 publications

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“…Both identification systems accurately identified L. adecarboxylata isolates. In literature, there are many reports that L. adecarboxylata was identified by automated identification systems and mass spectrometry [4,[6][7][8][9][10]. However, only two of these reports were confirmed by 16S rRNA gene sequencing, like our report [9,10].…”

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confidence: 52%

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A rarely isolated Gram-negative bacterium in microbiology laboratories: Leclercia adecarboxylata

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et al. 2018

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confidence: 52%

“…They also stated that fosfomycin resistance might be useful to differentiate L. adecarboxylata and E. coli [2]. Despite this knowledge, there were no information about fosfomycin resistance, or it was not tested in most of the studies [2][3][4][6][7][8][9][10]. One of our isolates was resistant to fosfomycin, whereas the other was susceptible.…”

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confidence: 89%

A rarely isolated Gram-negative bacterium in microbiology laboratories: Leclercia adecarboxylata

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et al. 2018

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“…The rapid horizontal spread of drug-resistant genes by plasmids is one of the reasons for the increasing number of multidrug resistant bacteria, however, the transfer range of bacteria species is unknown. Studies have confirmed that many countries and regions have isolated Gram-negative bacteria with mcr-1 gene (Fernandes et al, 2016;Hadjadj et al, 2017;Liu et al, 2017a), the bla NDM−1 gene (Wang et al, 2014;Michael et al, 2015;Zenati et al, 2016;Abderrahim et al, 2017;Madec et al, 2017;Riazzo et al, 2017;Yu et al, 2017), or both (Zheng et al, 2016;Liu et al, 2017b;Wang R. et al, 2018), from humans, the environment and animals. Whether isolates from antibiotics-free and depopulated areas, wildlife, carried mcr or bla NDM genes is undiscovered.…”

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confidence: 93%

Retrospective Screening and Analysis of mcr-1 and blaNDM in Gram-Negative Bacteria in China, 2010–2019

Fan

Duan

et al. 2020

Front. Microbiol.

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Currently, Gram-negative bacteria have developed multidrug and broad-spectrum drug resistance, and the numbers of species and strains carrying mcr or bla NDM genes are increasing. In this study, mcr-1 and bla NDM distribution of 12,858 Gramnegative bacteria isolated from wildlife, patients, livestock, poultry and environment in 14 provinces of China from 2010 to 2019 and the antibiotics resistance in regard to polymyxins (polymyxin B and colistin) and carbapenems of positive strains were investigated. A total of 70 strains of 10 species carried the mcr-1 gene, positive rates of patients, livestock and poultry, and environmental strains were 0.62% (36/5,828), 4.07% (29/712), 5.43% (5/92), respectively. Six strains of 3 species carrying the bla NDM gene all came from patients 0.10% (6/5,828). Two new mcr-1 gene variants (GenBank: MK965883, MK965884) were identified, one of which contains premature stop codon. The drug susceptibility results showed that all mcr-1 carriers were sensitive to carbapenems, among which, 66 strains were resistant and 4 were sensitive to polymyxins. The strains with the bla NDM gene had different degrees of resistance to carbapenems and were sensitive to polymyxins. The findings that species carrying mcr-1 or bla NDM genes were limited and mostly normal flora of opportunistic or low pathogenic organisms indicated that transfer of mcr-1 and bla NDM genes between bacteria was relatively limited in China. The none detection among wildlife compared with other sources supports the speculation that the emergence of and increase in polymyxins and carbapenem-resistant strains was mainly related to the selective pressure of antibiotics.

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“…Leclercia adecarboxylata, the only species of this genus whose complete genome sequences is available, has been described as an opportunistic pathogen and occasional carrier of bla NDM-1 (43). A few cases of L. adecarboxylata infection have been documented (44)(45)(46), and at least one report describes a case of hospital-acquired pneumonia likely due to L. adecarboxylata (47). In August 2016, bla KPC ϩ Leclercia sp.…”

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Genomic Analysis of Hospital Plumbing Reveals Diverse Reservoir of Bacterial Plasmids Conferring Carbapenem Resistance

Weingarten

Johnson

Conlan

et al. 2018

mBio

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The hospital environment is a potential reservoir of bacteria with plasmids conferring carbapenem resistance. Our Hospital Epidemiology Service routinely performs extensive sampling of high-touch surfaces, sinks, and other locations in the hospital. Over a 2-year period, additional sampling was conducted at a broader range of locations, including housekeeping closets, wastewater from hospital internal pipes, and external manholes. We compared these data with previously collected information from 5 years of patient clinical and surveillance isolates. Whole-genome sequencing and analysis of 108 isolates provided comprehensive characterization of bla KPC /bla NDM -positive isolates, enabling an in-depth genetic comparison. Strikingly, despite a very low prevalence of patient infections with bla KPC -positive organisms, all samples from the intensive care unit pipe wastewater and external manholes contained carbapenemase-producing organisms (CPOs), suggesting a vast, resilient reservoir. We observed a diverse set of species and plasmids, and we noted species and susceptibility profile differences between environmental and patient populations of CPOs. However, there were plasmid backbones common to both populations, highlighting a potential environmental reservoir of mobile elements that may contribute to the spread of resistance genes. Clear associations between patient and environmental isolates were uncommon based on sequence analysis and epidemiology, suggesting reasonable infection control compliance at our institution. Nonetheless, a probable nosocomial transmission of Leclercia sp. from the housekeeping environment to a patient was detected by this extensive surveillance. These data and analyses further our understanding of CPOs in the hospital environment and are broadly relevant to the design of infection control strategies in many infrastructure settings.IMPORTANCE Carbapenemase-producing organisms (CPOs) are a global concern because of the morbidity and mortality associated with these resistant Gramnegative bacteria. Horizontal plasmid transfer spreads the resistance mechanism to new bacteria, and understanding the plasmid ecology of the hospital environment can assist in the design of control strategies to prevent nosocomial infections. A 5-year genomic and epidemiological survey was undertaken to study the CPOs in the patient-accessible environment, as well as in the plumbing system removed from the patient. This comprehensive survey revealed a vast, unappreciated reservoir of CPOs in wastewater, which was in contrast to the low positivity rate in both the patient population and the patient-accessible environment. While there were few patient-environmental isolate associations, there were plasmid backbones common to both populations. These results are relevant to all hospitals for which CPO colonization may not yet be defined through extensive surveillance.

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First report of NDM-1-producing clinical isolate of Leclercia adecarboxylata in Spain

Cited by 20 publications

References 27 publications

A rarely isolated Gram-negative bacterium in microbiology laboratories: Leclercia adecarboxylata

A rarely isolated Gram-negative bacterium in microbiology laboratories: Leclercia adecarboxylata

Retrospective Screening and Analysis of mcr-1 and blaNDM in Gram-Negative Bacteria in China, 2010–2019

Genomic Analysis of Hospital Plumbing Reveals Diverse Reservoir of Bacterial Plasmids Conferring Carbapenem Resistance

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