First Report of Natural Infection by Capsicum Chlorosis Virus on Amaryllis (<i>Hippeastrum hybridum</i>) Plants from India

Basavaraj, Y. B.; Siwach, Jyoti; Kumar, Ashwani; Bhattarai, Ajay; Qayoom, Nayeem; Jain, Rakesh

doi:10.1094/pdis-03-20-0537-pdn

Cited by 7 publications

(5 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For instance, Chen et al (2012) [7] conducted serological detection on infected calla lily samples using antiserum against CaCV and monoclonal antibodies against WSMoV, with both yielding positive reactions. [26] , Basvaraj et al (2020) [1] , and Chen et al (2022) [6] .…”

Section: Serological Detectionmentioning

confidence: 98%

Molecular characterization of bell pepper (Capsicum annuum L.) infecting Capsicum chlorosis orthotospovirus

Nandini,

Ruth,

Babu

2024

Int. J. Adv. Biochem. Res.

View full text Add to dashboard Cite

In 2023, surveys were carried out in key bell pepper cultivation regions of the Andhra Pradesh district to gather virus isolates for analysis. The virus responsible was identified based on its symptoms, how it spreads, and through serological and molecular investigations. Affected plants exhibited stunted growth, dwarfism, and concentric chlorotic ringspot symptoms on their leaves. Infected bell pepper fruits showed deformities with nailhead symptoms, or ripe fruits displayed pale yellow ringspots of various colours. Samples showing characteristic symptoms were gathered and employed to inoculate healthy bell pepper plants. Serological testing using Tospo (I, II, III) and CaCV antisera via DAS-ELISA identified the virus as Capsicum chlorosis orthotospovirus (CaCV), as it reacted solely with CaCV antisera. Further molecular analysis, including RT-PCR, confirmed the virus's identity as Capsicum chlorosis orthotospovirus.

show abstract

Section: Serological Detectionmentioning

confidence: 98%

Molecular characterization of bell pepper (Capsicum annuum L.) infecting Capsicum chlorosis orthotospovirus

Nandini,

Ruth,

Babu

2024

Int. J. Adv. Biochem. Res.

View full text Add to dashboard Cite

show abstract

“…CaCV belongs to the tospovirus serogroup IV (McMichael et al., 2002 ; Kunkalikar et al., 2007 ) and can be serologically detected using polyclonal and monoclonal antibodies against tospoviruses of this group, although conclusive identification of the virus needs further confirmation by molecular methods (McMichael et al., 2002 ; Zheng et al., 2010 ; Manyam and Byadgi, 2014 ; Basavaraj et al., 2020 ; Hassani‐Mehraban et al., 2016 , EPPO/OEPP, 2020 ). Double‐antibody sandwich ELISA kits are commercially available for the detection of CaCV.…”

Section: Pest Categorisationmentioning

confidence: 99%

“…In southern China, CaCV was reported as the causal agent of a widely occurring disease of peanuts, with an incidence up to 20% in some fields, where large yield losses were observed especially in plants infected at early growing stages (Chen et al., 2007 ). In India, disease incidence higher than 20% and up to 36% was recorded in chili pepper and ornamental amaryllis ( Hippeastrum hybridum ) cultivations, respectively (Krishnareddy et al., 2008 ; Basavaraj et al., 2020 ).…”

Section: Pest Categorisationmentioning

confidence: 99%

“…(2012) Basavaraj et al. ( 2020 ) CABI (online) CABI (online) CABI (online) North America United States Hawaii Present CABI (online) Oceania Australia Queensland Western Australia East Kimberley Present Present Present CABI (online) CABI (online) Jones and Sharman (2005) …”

Section: Appendix B – Distribution Of Capsicum Chlorosis Virusmentioning

confidence: 99%

“…When these primers are used, final identification of CaCV needs cloning and sequencing or direct sequencing of the obtained PCR amplicons (Zheng et al, 2008;Haokip et al, 2016;Yin et al, 2016). Specific primers for RT-PCR-based identification of CaCV have been designed and efficiently applied in field surveys for the specific amplification of CaCV cDNAs and for the conclusive identification of the virus by more detailed sequence analyses (Krishnareddy et al, 2008;Yin et al, 2016;Basavaraj et al, 2020;Haokip et al, 2021). Hassani-Mehraban et al (2016) designed generic and species-specific primers to detect tospoviruses in all the recognised tospovirus clades (Oliver and Whitfield, 2016;Hassani-Mehraban et al, 2019).…”

Section: Detection and Identification Of The Pestmentioning

confidence: 99%

See 2 more Smart Citations

Pest categorisation of Capsicum chlorosis virus

Bragard¹,

Baptista²,

Chatzivassiliou³

et al. 2022

EFS2

View full text Add to dashboard Cite

The EFSA Panel on Plant Health conducted a pest categorisation of Capsicum chlorosis virus (CaCV) for the EU territory. The identity of CaCV, a member of the genus Orthotospovirus (family Tospoviridae ), is established and reliable detection and identification methods are available. The pathogen is not included in the EU Commission Implementing Regulation 2019/2072. CaCV has been reported in Australia, China, India, Iran, Taiwan, Thailand and USA (Hawaii). In the EU, it has been reported once in Greece (Crete Island). The NPPO of Greece reported that CaCV is no longer present in Greece. CaCV infects plant species in the family Solanaceae (i.e. pepper, tomato) and several species of other families, including ornamentals. It may induce severe symptoms on its hosts, mainly on leaves and fruits, which may become unmarketable. The virus is transmitted in a persistent propagative mode by the thrips Ceratothripoides claratris, Frankliniella schultzei, Microcephalothrips abdominalis and Thrips palmi. C. claratris and T. palmi are EU quarantine pests. M . abdominalis is known to be present in several EU member states and it is not regulated in the EU. Plants for planting, parts of plants, fruits and cut flowers of CaCV hosts, and viruliferous thrips were identified as the most relevant pathways for the entry of CaCV into the EU. Cultivated and wild hosts of CaCV are distributed across the EU. Should the pest enter and establish in the EU territory, impact on the production of cultivated hosts is expected. Phytosanitary measures are available to prevent entry and spread of the virus in the EU. CaCV fulfils the criteria that are within the remit of EFSA to assess for it to be regarded as a potential Union quarantine pest.

show abstract

Tospovirus Diseases of Ornamental Plants: Characterization, Identification, Diagnosis and Management

Karthikeyan

Nagendran

Kumari

et al. 2021

Virus Diseases of Ornamental Plants

View full text Add to dashboard Cite

First Report of Natural Infection by Capsicum Chlorosis Virus on Amaryllis (Hippeastrum hybridum) Plants from India

Cited by 7 publications

References 7 publications

Molecular characterization of bell pepper (Capsicum annuum L.) infecting Capsicum chlorosis orthotospovirus

Molecular characterization of bell pepper (Capsicum annuum L.) infecting Capsicum chlorosis orthotospovirus

Pest categorisation of Capsicum chlorosis virus

Tospovirus Diseases of Ornamental Plants: Characterization, Identification, Diagnosis and Management

Contact Info

Product

Resources

About