First Report of Maize White Spot Disease Caused by <i>Pantoea ananatis</i> in China

Cui, Lina; Zou, Chengjia; Zhang, Zhenyu; Duan, Luyao; Ji-mei, Huang; Wang, Liming; Xiao, Wang; Yang, Xiaorong; Xiang, Yunjia; Li, Wenyi; Li, Xiao; Zhang, Haiyan

doi:10.1094/pdis-01-22-0152-pdn

Cited by 5 publications

(1 citation statement)

References 8 publications

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“…Maize white spot, caused by the bacterium Pantoea ananatis, emerged as one of the most important foliar maize disease widely distributed in America (Kistner et al, 2021), Africa (Goszczynska et al, 2007), Europe (Krawczyk et al, 2010) and Asia (Cui et al, 2022), and is considered a potential threat to maize production in regions where high humidity and low night time temperatures are prevalent during the growing season. Symptoms are initially expressed as aqueous lesions in the basal leaves, rapidly spreading to the plant with a chlorotic appearance at more advanced stages of the disease, causing drastically reducing cycle and photosynthetic area of the affected plants (Derera et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Biocontrol potential of actinobacteria against Pantoea ananatis, the causal agent of maize white spot disease

et al. 2023

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Pantoea ananatis is the causal agent of maize white spot, a foliar disease responsible for significant maize yield reduction worldwide, especially in Brazil. In general, the maize foliar diseases control involves the adoption of resistant genotypes and pesticides application. However, the use of agrochemicals can significantly cause increase production costs, damage to human health and negative environmental impacts. In this sense, the use of biological control agents has been considered among the most promising eco-friendly technologies for sustainable agriculture. Actinobacteria, particularly of Streptomyces genus, has been widely recognized as agroindustrially important microorganism due to its potential in producing diverse range of secondary metabolites, including antibiotics and enzymes. Thus, the aim of this work is to characterize and to evaluate the potential of soil actinobacteria for P. ananatis control. We observed that 59 actinobacteria strains (85%) exhibited proteolytic or chitinolytic activity. Only the strains Streptomyces pseudovenezuelae ACSL 470, that also exhibited high proteolytic activity, S. novaecaesareae ACSL 432 and S. laculatispora ACP 35 demonstrated high or moderate antagonist activity in vitro against P. ananatis. Temporal analysis of metabolites produced by these strains growth in different liquid media indicated greater antibacterial activity at 72 h. In this condition, chromatographic and mass spectrometry analysis revealed that S. pseudovenezuelae ACSL 470 strain produced neomycin, an aminoglycoside antibiotic that displayed high bactericidal activity in vitro against P. ananatis. This is the first report of actinobacteria acting as potential microbial antagonists for P. ananatis control. Further studies are needed to determine the control efficacy of maize white spot disease by Streptomyces strains or their metabolites in greenhouse and field conditions.

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Section: Introductionmentioning

confidence: 99%

Biocontrol potential of actinobacteria against Pantoea ananatis, the causal agent of maize white spot disease

et al. 2023

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First Report of Pantoea ananatis Causing Leaf Spot Disease in Tobacco (Nicotiana tabacum) in China

Gao,

Liu,

Anane

et al. 2024

Plant Disease

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First Report of Bacterial Leaf Streak in Sweet Corn Caused by Pantoea ananatis in Southeast China

Zhang

et al. 2023

Plant Disease

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Sweet corn (Zea mays convar. saccharata var. rugosa) is a popular vegetable crop in southeast China. During the spring seasons of 2018-2021, a serious outbreak of bacterial leaf streak was observed in sweet corn variety Yuetian28 in the field in Guangzhou, Guangdong Province. The disease incidence was 50%-70%. Infected leaves initially displayed long, chlorotic streaks parallel to veins at the V5-V6 stage, and then turned white or brown and dried out over the course of disease development. In severe infections, leaf lesion coalesced to form large irregular blight areas (Fig. S1A). To investigate this disease, we collected 0.5 cm2 samples of infected leaves from four plants after surface sterilization and rinsed them three times with sterile distilled water. We placed all leaf samples on nutrient agar (NA) medium and incubated them at 28℃ for 48 hours. Bright-yellowish colonies were observed near the edges of the samples. We picked the colonies and re-streaked them onto NA medium three times to obtain pure cultures. Four isolates, GZ2201, GZ2202, GZ2203, and GZ2204, were selected for further study. All isolates were gram-negative rods and were negative for oxidase, urease, nitrate reductase reactions, and gelatin liquefaction. They were positive for catalase, citrate utilization, indole production, and the Voges-Proskauer test. We sequenced the 16S rDNA, rpoB, leuS, and gyrB sequences using previously reported primers (Brady et al. 2008) and deposited the sequences in GenBank (accession nos. ON740665 to ON740668 for 16S rDNA; ON755167 to ON755170 for rpoB; ON755171 to ON755174 for leuS; and OP227136 to OP227139 for gyrB). The sequences share >98% identity with sequences from Pantoea ananatis type strain LMG2665 (GenBank JFZU01) indicating that the causal pathogen of bacteria leaf streak of sweet corn is P. ananatis (Fig. S1B). Phylogenetic analysis of gyrB, leuS, and rpoB concatenated sequence showed that the four isolates clustered with P. ananatis (Fig S2). To test the pathogenicity of the isolates of P. ananatis on the sweet corn variety Yuetian28, we inoculated plants at the V3 stage by syringe infiltration of bacterial suspension (108 CFU/ml) (Kini et al. 2020) or sterile distilled water as a negative control. Inoculated plants were placed in a growth chamber at 28 ℃, 60% relative humidity, 16-h/8-h light-dark cycle. After 7 days of incubation, chlorotic streaks resembling the original symptoms developed on inoculated plants (Fig. S1D), while control plants remained symptomless (Fig. S1C). We successfully re-isolated bacteria from the inoculated plants and confirmed their identity by sequencing of 16S rDNA, rpoB, leuS, and gyrB. P. ananatis was previously reported to cause leaf spot disease in maize grown in Argentina, Ecuador, and China (Alippi et al. 2010; Toaza et al. 2021; Cui et al. 2022). To our knowledge, this is the first report of P. ananatis causing leaf streak in sweet corn in southeast China. Further research on P. ananatis management is needed to help control disease spread.

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First Report of Maize White Spot Disease Caused by Pantoea ananatis in China

Cited by 5 publications

References 8 publications

Biocontrol potential of actinobacteria against Pantoea ananatis, the causal agent of maize white spot disease

Biocontrol potential of actinobacteria against Pantoea ananatis, the causal agent of maize white spot disease

First Report of Pantoea ananatis Causing Leaf Spot Disease in Tobacco (Nicotiana tabacum) in China

First Report of Bacterial Leaf Streak in Sweet Corn Caused by Pantoea ananatis in Southeast China

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