First Report of Leek Yellow Stripe Virus, Onion Yellow Dwarf Virus, and the Putative Allium Polerovirus A in Elephant Garlic (Allium ampeloprasum) in Brazil
Abstract:Five elephant garlic plants (Allium ampeloprasum L.) showing leaf symptoms of chlorotic streaks and mosaic (Figure 1A and B) were collected, in September 2021, in an experimental area in municipality of Rio do Sul (27°11'07"S, 49°39'39"W), State of Santa Catarina, Brazil. Total RNA was extracted using TRIzol® reagent (Invitrogen, USA), according to the manufacturer’s instructions to investigate viral infection. The RNA from all five plants were pooled into a single sample for cDNA library construction with the… Show more
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