First Report of Leaf Spot of Chlorophytum comosum caused by Thielavia terrestris from Pakistan

Ashraf, Khadija; Nawaz, Maryam; Yousaf, Nousheen; Afshan, Najam‐ul‐Sehar

doi:10.33804/pp.006.03.4313

Cited by 4 publications

(4 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The polymerase chain reaction (PCR) products electrophoresed through 1% agarose gel were visualized with ultraviolet light after ethidium bromide staining. All the isolates yielded a 750-bp band that corresponded to R. solanacearum (Anwar et al 2022;Ashraf et al 2022).…”

Section: Molecular Confirmationmentioning

confidence: 99%

Evaluation of virulence among Pakistani isolates of Ralstonia solanacearum inducing bacterial wilt in chilies across different agro-ecological zones

Aslam,

Mukhtar

2024

Bragantia

View full text Add to dashboard Cite

No information regarding pathogenic variability among different isolates of Ralstonia solanacearum infecting chili from different agro-ecological zones of Pakistan with varying climatic and edaphic factors is available. Therefore, in the present study, variations were observed in biovar distribution, hypersensitive response, growth and virulence among 114 isolates of R. solanacearum collected from eight agro-ecological zones of Pakistan. Out of 114 R. solanacearum isolates, 81% were identified as Biovar III, while the remaining 19% were recognized as Biovar IV. Of all the 114 isolates of R. solanacearum, 77% showed positive hypersensitive response and mucoid growth, while 23% isolates gave negative hypersensitive response with non-mucoid growth. Out of 114 isolates of R. solanacearum consisting of Biovar III and IV, 22.8% were found avirulent, 25.4% weakly virulent, 29.8% virulent, and the remaining 21.9% were highly virulent. Variations among 114 R. solanacearum isolates were also observed in four provinces of the country. Among 92 R. solanacearum Biovar III isolates, 21.7% were identified as avirulent, 25% weakly virulent, 34.4% virulent, and 22.8% were highly virulent in the eight agro-ecological zones of the country. Similarly, out of 22 R. solanacearum Biovar IV isolates, 27.3% were detected as avirulent, weakly virulent and virulent, while 18.2% isolates were found highly virulent. The isolates having non-mucoid growth and negative hypersensitive response were found avriulent, while those with mucoid growth and positive hypersensitive response were weakly virulent to highly virulent. The information will help design control strategies accordingly and develop resistant cultivars against the bacterium.

show abstract

Section: Molecular Confirmationmentioning

confidence: 99%

Evaluation of virulence among Pakistani isolates of Ralstonia solanacearum inducing bacterial wilt in chilies across different agro-ecological zones

Aslam,

Mukhtar

2024

Bragantia

View full text Add to dashboard Cite

show abstract

“…The annealing temperature was adjusted according to the composition of the oligonucleotide sequence. The PCR products electrophoresed through a 1% agarose gel were visualized with ultraviolet light after ethidium bromide staining (Anwar et al 2022;Ashraf et al 2022). All the isolates yielded a 750-bp band that corresponded to R. solanacearum.…”

Section: Molecular Confirmationmentioning

confidence: 99%

Distributional spectrum of bacterial wilt of chili incited by Ralstonia solanacearum in Pakistan

Aslam

Mukhtar

2023

Bragantia

View full text Add to dashboard Cite

The production of chili is seriously threatened by bacterial wilt incited by Ralstonia solanacearum in Pakistan. As the information about the distribution and prevalence of the disease and the pathogen is scarce, the present study was performed to ascertain the prevalence, occurrence and distribution of R. solanacearum in different regions of chili cultivation. The results showed differences in the incidence and prevalence of bacterial wilt all over the country. Overall, an incidence of 10% and prevalence of 76% of the disease was observed in the country. The highest incidence of bacterial wilt was observed in the province of Sindh whereas it was the lowest in Baluchistan. The prevalence of the disease followed the similar trend in the provinces. As regards agroecological zones, the highest incidence was recorded in Indus delta followed by sandy deserts whereas it was the lowest in western dry mountains. Almost the same tendency was observed regarding prevalence in the eight agroecological zones. In case of districts, the disease incidence was the maximum in Badin while it was the minimum in the district of Loralai. On the other hand, the disease prevalence was the maximum (100%) in the districts of Badin, Mirpurkhas, and Thatta and the minimum (60%) in the districts of Barkhan, Karak, and Loralai. Of the total 114 isolates of R. solanacearum, 81% were confirmed as Biovar 3 whereas the rest 19% isolates were identified as Biovar 4. The findings will help the farmers to design disease management programs accordingly to avert yield losses.

show abstract

“…DNA was extracted immediately using a standard Proteinase-K method. The final DNA pellets were re-suspended in 10 mM Tris-HCl buffer (pH 8) and were stored at-80°C (Anwar et al, 2022;Ashraf et al, 2022).…”

Section: Molecular Confirmation Extraction Of Dnamentioning

confidence: 99%

“…The PCR product was mixed with 6× loading dye and was loaded on the agarose gel. The resulting gel image was captured by using the gel documentation unit (Anwar et al, 2022;Ashraf et al, 2022).…”

Section: Gel Electrophoresismentioning

confidence: 99%

Incidence, Characterization and Pathogenic Variability of Fusarium oxysporum in the Punjab Province of Pakistan

Zeeshan

Mukhtar

Inam–ul-Haq

et al. 2023

Int. J. Phytopathol.

View full text Add to dashboard Cite

The present studies were conducted to determine the incidence of wilt caused by Fusarium oxysporum in the major chili-growing districts of Punjab, characterization of Fusarium isolates, and assessment of their pathogenic variability. The overall incidence of Fusarium wilt in the five major surveyed districts of the Punjab province was 9.2% in the year 2015-16 and 9.3% in the year 2016-17. The maximum disease incidence of 10.04% was recorded in district Multan in the year 2015-16 while it was the minimum (7.06%) in district Sahiwal. A similar trend was observed in the disease incidences during the year 2016-17. The disease incidences varied among different localities of the five districts. In total, 92 isolates were recovered from the chili crop in Punjab. All the recovered purified isolates were morphologically characterized using different parameters like colony color, growth habit, pigmentation, days to fill 9-cm-dish, concentric rings, size and shape of macroconidia and microconidia, phialide, septation in macroconidia, diameter, and formation of chlamydospores and interseptal distance. All the isolates were confirmed as Fusarium oxysporum. Based on the pathogenicity test, plants showing more than 75% wilting were rated as highly pathogenic, plants showing 50.1-75% wilting were pathogenic, plants showing 25.1-50% wilting were categorized as moderately pathogenic, and plants showing 0.1-25% wilting were graded as weakly pathogenic. The highly pathogenic and pathogenic isolates were subjected to molecular studies and all the isolates made a band of 550-600 on the gel. All the isolates showed 99-100% similarity with already reported Fusarium species. The phylogenetic tree showed a close relationship between all the isolates

show abstract

First Report of Leaf Spot of Chlorophytum comosum caused by Thielavia terrestris from Pakistan

Cited by 4 publications

References 9 publications

Evaluation of virulence among Pakistani isolates of Ralstonia solanacearum inducing bacterial wilt in chilies across different agro-ecological zones

Evaluation of virulence among Pakistani isolates of Ralstonia solanacearum inducing bacterial wilt in chilies across different agro-ecological zones

Distributional spectrum of bacterial wilt of chili incited by Ralstonia solanacearum in Pakistan

Incidence, Characterization and Pathogenic Variability of Fusarium oxysporum in the Punjab Province of Pakistan

Contact Info

Product

Resources

About