First report of larval stages of<i>Fasciola hepatica</i>in a wild population of<i>Pseudosuccinea columella</i>from Cuba and the Caribbean

Gutierrez, Alain; Vázquez, Antonio A.; Hevia, Yosvania; Sánchez, Jorge; Correa, Ana C.; Hurtrez‐Boussès, Sylvie; Pointier, Jean‐Pierre; Thèron, André

doi:10.1017/s0022149x10000350

Cited by 28 publications

(28 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The other lymnaeid species occurring in Cuba, P. columella, has been found only twice infected in the field with a prevalence of 3 % (Gutiérrez et al 2011) and 2 % (author unpublished data). G. cubensis is the most common lymnaeid species in bovine grazing areas which may be the reason of having found infected individuals in several localities.…”

Section: Discussionmentioning

confidence: 99%

“…The only two known lymnaeid species are Galba cubensis and Pseudosuccinea columella, both have experimentally been confirmed to transmit F. hepatica, and the former is considered the main intermediate host (Gutiérrez et al 2011;Vázquez et al 2014). However, although populations of G. cubensis have been found in sites with fascioliasis outbreaks in Cuba (Ferrer et al 1985), their infection rates have not been investigated.…”

Section: Introductionmentioning

confidence: 95%

See 1 more Smart Citation

Natural prevalence in Cuban populations of the lymnaeid snail Galba cubensis infected with the liver fluke Fasciola hepatica: small values do matter

et al. 2015

View full text Add to dashboard Cite

Natural infections of lymnaeid snails by Fasciola hepatica are of primary importance to study transmission. Also, infected snails in the field can be used to explore the existing compatibility in host-parasite interactions. This paper aimed to describe the infection rate of Galba cubensis populations in fasciolosis transmission areas. Eight sites were sampled in western Cuba and 24 infected snails at six sites were found. The mean prevalence was 2.94% and the maximum value was 11.4%. The intensity of parasite infection was assessed as the number of rediae inside a single snail. High variation within the sites examined was observed, but a maximum of 76 rediae was recovered from one individual. Although the presence of two other trematode families (Schistosomatidae and Paramphistomatidae) was discovered in dissected individuals, no co-infection with F. hepatica was observed. This is the first time a study of natural prevalence of F. hepatica infection is carried out in Cuba, considered a hyper endemic country for bovine fasciolosis. Our results suggest that fasciolosis transmission may occur even when the number of infected snails remains relatively low.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

Natural prevalence in Cuban populations of the lymnaeid snail Galba cubensis infected with the liver fluke Fasciola hepatica: small values do matter

et al. 2015

View full text Add to dashboard Cite

show abstract

“…In Central and South America, P. columella enlarged its distribution area and was reported from Cuba (Gutiérrez, Pointier, Yong, Sanchez, & Theron, 2003), Guadalupe island (Durand et al, 2002), Puerto Rico, Mexico, Jamaica, Guatemala, Costa Rica, Panamá, Ecuador, Brasil, Paraguay, Argentina (Paraense, 1982), Colombia (Salazar, Estrada, & Velásquez, 2006), Venezuela (Malek, & Chrosciechowski, 1964), Uruguay (Heinzen, Castro, Pepe, & Ibarburu, 1994) and Perú (Larrea, Flórez, Vivar, Huamán, & Velásquez, 2007). The role of P. columella as intermediate host of F. hepatica was confirmed in Cuba (Gutiérrez et al, 2011), Colombia (Salazar et al, 2006), Brazil (Coelho, Lima, & Guimaraes, 2009), Argentina (Prepelitchi et al, 2003), New Zealand (Harris, & Charleston, 1976), Australia (Boray et al, 1984) and France (Pointier, Coustau, Rondelaud, & Theron, 2007) among others.…”

mentioning

confidence: 92%

Efecto de la espirulina (Arthrospira platensis) (Oscillatoriales: Cyanobacteria) en la cría experimental de Pseudosuccinea columella (Basommatophora: Lymnaeidae)

Prepelitchi

Pujadas

Wisnivesky‐Colli

2015

RBT

View full text Add to dashboard Cite

Snails of the family Lymnaeidae, as Pseudosuccinea columella, are the intermediate hosts of Fasciola hepatica, the causative agent of fasciolosis in human and livestock all over the world. A thorough knowledge of snail biology is essential for describing the transmission dynamics and for controlling this disease. Since food quality has had a significant effect on snail growth, fecundity and fertility, in this study we evaluated the use of spirulina (Arthrospira platensis) as a food resource for the artificial breeding of P. columella, an invasive snail and the main intermediate host of F. hepatica in Northeastern Argentina. The main purpose was to measure the effect of spirulina on fitness parameters such as survival rate, growth rate, size at first reproduction, lifetime fecundity and viable offspring. A total of 20 676 newly-laid F 2 eggs were used; half of them were fed with lettuce (treatment L) and the other half with lettuce plus spirulina (treatment L+S). In comparison with P. columella snails fed only with lettuce, we found that P. columella fed with lettuce plus spirulina: 1) showed higher survival rates, 2) grew faster and showed higher growth increments, 3) attained sexual maturity earlier in time (L+S:60 days vs. L:120 days) and at a smaller size (L+S:4.8mm vs. L:8.2mm), 4) had a longer reproductive period (L+S:150 days vs. L:90 days), 5) produced a higher number of eggs/snail (L+S:29.6 vs. L:13.3), and 6) showed a higher offspring hatching rate (L+S:70% vs. L:40%). The supplementation of P. columella diet with commercial spirulina enhances it fitness and improved the artificial breeding of this species. Spirulina may have a direct positive effect on P. columella development by consuming it, along with an indirect positive effect by improving the water quality. This rearing technique provided large number of reproducing adults and a continuous production of offspring, which are essential for developing future experimental studies in order to improve our knowledge on P. columella biology. Rev. Biol. Trop. 63 (2): 479-489. Epub 2015 June 01.

show abstract

“…Fluctuations in the number of Lymnaea stagnalis haemocytes in the haemolymph during infection by Trichobilharzia ocellata have been observed ( VAN DER KNAAP et al, 1987;AMEN et al, 1991). Other factors that also affect the success of the intramollusc trematode are age, strain or geographical origin of the lymnaeids (GUTIÉRREZ et al, 2011;COELHO et al, 2009;DREYFUSS et al, 2012). When infected, lymnaeids change biology which may reflect on the survival rate (MENDES et al, 2008;VIGNOLES et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Dynamics of haemocytes from Pseudosuccinea columella circulating infected by Fasciola hepatica

Ribeiro

Coaglio

Oliveira

et al. 2017

Rev. Bras. Parasitol. Vet.

View full text Add to dashboard Cite

The lymnaeids are important in the epidemiology of Fasciola hepatica, a neglected and endemic zoonosis. The interaction between the internal defense system of Pseudosuccinea columella and F. hepatica has been little studied. In the present study the effect of infection by F. hepatica on P. columella circulating haemocytes was investigated. Changes in the average number of total circulating haemocytes have been observed at 30 minutes post-infection and 1, 7, 10, 14, 21, 28 and 50 days post-infection (dpi). Miracidia were observed head-foot and mantle at 30 minutes post-infection. Miracidia/Sporocysts in the mantle skirt 1 dpi, and fully formed sporocysts were observed in the head-foot at 7 dpi. Rediae became evident at 10 dpi and were located between the haemocoel and the muscles from 14 dpi; 50 dpi, the rediae in the digestive gland contained cercariae. The statistical analysis of the total haemocytes of P. columella infected by F. hepatica showed significant differences on the 30 minutes post-infection and 1, 14, 21, and 28 dpi in comparison to uninfected molluscs (0 dpi). Therefore, the interference observed on the internal defence system of P. columella may have direct association with the development of F. hepatica.Keywords: Fasciola hepatica, haemocytes, Pseudosuccinea columella. ResumoOs limnaeideos são importantes na epidemiologia de Fasciola hepatica, uma zoonose negligenciada e endêmica. A interação entre o sistema interno de defesa de Pseudosuccinea columella e F. hepatica tem sido pouco estudada. No presente estudo, investigou-se o efeito da infecção por F. hepatica nos hemócitos circulantes de P. columella. Alterações no número médio de hemócitos circulantes foram observadas aos 30 minutos e 1, 7, 10, 14, 21, 28 e 50 dias após a infecção (dpi). Miracídios foram observados na região cefalopodal e manto aos 30 minutos após a infecção. Miracídio/esporocistos foram observados no colar do manto ao 1 dpi, e esporocistos totalmente formados na região cefalopodal aos 7 dpi. Rédias tornam-se evidentes aos 10 dpi entre a hemocele e músculos a partir de 14 dpi; e rédias com cercárias próximas a glândula digestiva aos 50 dpi. A análise estatística dos hemócitos totais de P. columella infectados por F. hepatica demonstrou diferenças significativas nos 30 minutos pós-infecção e 1, 14, 21 e 28 dpi em comparação aos moluscos não infectados (0 dpi). Portanto, a interferência observada no sistema de defesa interna de P. columella pode ter associação direta com o desenvolvimento de F. hepatica.

show abstract

First report of larval stages ofFasciola hepaticain a wild population ofPseudosuccinea columellafrom Cuba and the Caribbean

Cited by 28 publications

References 17 publications

Natural prevalence in Cuban populations of the lymnaeid snail Galba cubensis infected with the liver fluke Fasciola hepatica: small values do matter

Natural prevalence in Cuban populations of the lymnaeid snail Galba cubensis infected with the liver fluke Fasciola hepatica: small values do matter

Efecto de la espirulina (Arthrospira platensis) (Oscillatoriales: Cyanobacteria) en la cría experimental de Pseudosuccinea columella (Basommatophora: Lymnaeidae)

Dynamics of haemocytes from Pseudosuccinea columella circulating infected by Fasciola hepatica

Contact Info

Product

Resources

About