First Report of Isolation and Genome Sequence of L. petauri Strain from a Rainbow Trout Lactococcosis Outbreak

Kotzamanidis, Christos; Malousi, Andigoni; Bitchava, Konstantina; Vafeas, George; Chatzidimitriou, Dimitrios; Skoura, Lemonia; Papadimitriou, E; Chatzopoulou, Fani; Ζdragas, Αntonios

doi:10.1007/s00284-020-01905-8

Cited by 25 publications

(18 citation statements)

References 28 publications

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“…The MLST analysis clustered 86 L. garvieae isolates into 32 distinct STs, with 5 CCs and 18 singletons, which is consistent with the population structure in isolates from other hosts ( 37, 38, 43 ), the environment, or foods ( 44 ). All strains, except Hebei-B-22, were new STs and phylogenetically close to each other.…”

Section: Discussionsupporting

confidence: 66%

Comparative genomic analyses of Lactococcus garvieae isolated from bovine mastitis in China

Lin

Han

Barkema

et al. 2022

Preprint

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Lactococcus garvieae is an emerging zoonotic pathogen, but there are few reports regarding bovine mastitis. The recent prevalence of L. garvieae poses an increasing disease threat and global public health risk. A total of 39 L. garvieae isolates were obtained from 2899 bovine clinical mastitis milk samples in 6 provinces of China from 2017 to 2021. Five clonal complexes were determined from 32 MLST types of L. garvieae; ST46 was the predominant sequence type and 13 novel MLST types were identified. All isolates were resistant to chloramphenicol and clindamycin, but susceptible to penicillin, ampicillin, amoxicillin-clavulanic acid, imipenem, ceftiofur, enrofloxacin, and marbofloxacin. Based on genomic analyses, L. garvieae had 6310 genes, including 1015, 3641 and 1654 core, accessory and unique genes. All isolates had virulence genes coding for collagenase, fibronectin-binding protein, Glyceraldehyde-3-phosphate dehydrogenase, superoxide dismutase and NADH oxidase. Most isolates had lsaD and mdtA AMR genes. Based on COG results, the functions of defense, transcription and replication, recombination and repair were enhanced in unique genes, whereas functions of translation, ribosomal structure and biogenesis were enhanced in core genes. The KEGG functional categories enriched in unique genes included human disease and membrane transport, whereas COG functional categories enriched in core genes included energy metabolism, nucleotide metabolism and translation. No gene was significantly associated with host specificity. In addition, core genome SNPs analysis suggested potential host adaptation of some isolates in several sequence types. Therefore, this study characterized L. garvieae isolated from mastitis and assessed host adaptation of L. garvieae to various hosts.

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Section: Discussionsupporting

confidence: 66%

Comparative genomic analyses of Lactococcus garvieae isolated from bovine mastitis in China

Lin

Han

Barkema

et al. 2022

Preprint

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“…Genomic analysis reassigned L. garvieae subgroup A strains to the recently described species L. petauri ( 3 ). Consequently, both species can be considered the etiological agents of lactococcosis in fish ( 4 , 5 ) and emerging opportunistic zoonotic pathogens ( 6 – 8 ). The isolation of both species from a variety of food ( 9 – 11 ) also implicates a contribution to the quality and typicity of various traditional food products ( 10 ).…”

Section: Announcementmentioning

confidence: 99%

Genome Sequences of Lactococcus garvieae and Lactococcus petauri Strains Isolated from Traditional Montenegrin Brine Cheeses

Martinović

Rosel

Nisic

et al. 2021

Microbiol Resour Announc

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“…Although the causative agent was initially identified as L. garvieae using phenotypic, biochemical and conventional L. garvieae PCR, one of the isolates was reclassified as L. petauri sp. nov in a retrospective study using comparative genomic and phylogenetic analysis (Kotzamanidis et al, 2020). In the United States, a recent outbreak of piscine lactococcosis caused by L. petauri resulted in >50% mortality in some systems and the culling of over 3.2 million of farmed rainbow trout in California (Shahin et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Development of a quantitative polymerase chain reaction assay for detection of the aetiological agents of piscine lactococcosis

Shahin

Mukkatira

Yazdi

et al. 2022

Journal of Fish Diseases

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Piscine lactococcosis is an emergent bacterial disease that is associated with high economic losses in many farmed and wild aquatic species worldwide. Early and accurate detection of the causative agent of piscine lactococcosis is essential for management of the disease in fish farms. In this study, a TaqMan quantitative polymerase chain reaction (qPCR) targeting the 16S-23S rRNA internal transcribed spacer region was developed and validated. Validation of the qPCR was performed with DNA of previously typed L. petauri and L. garvieae recovered from different aquatic hosts from distinct geographical locations, closely related bacterial species and common pathogens in trout aquaculture. Further diagnostic sensitivity and specificity was investigated by screening of fish, water and faecal samples. The developed qPCR assay showed high specificity, sensitivity and accuracy in detection of L. petauri and L. garvieae with lack of signals from non-target pathogens, and in screening of rainbow trout (Oncorhynchus mykiss) posterior kidney and environmental samples. The detection limit of the qPCR was four amplicon copies. Moreover, the sensitivity of the qPCR assay was not affected by presence of non-target DNA from either fish or environmental samples. The robustness, specificity and sensitivity of the developed qPCR will facilitate fast and accurate diagnosis of piscine lactococcosis to establish appropriate control measures in fish farms and aquaria.

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First Report of Isolation and Genome Sequence of L. petauri Strain from a Rainbow Trout Lactococcosis Outbreak

Cited by 25 publications

References 28 publications

Comparative genomic analyses of Lactococcus garvieae isolated from bovine mastitis in China

Comparative genomic analyses of Lactococcus garvieae isolated from bovine mastitis in China

Genome Sequences of Lactococcus garvieae and Lactococcus petauri Strains Isolated from Traditional Montenegrin Brine Cheeses

Development of a quantitative polymerase chain reaction assay for detection of the aetiological agents of piscine lactococcosis

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