First Report of<i>Xylella fastidiosa</i>in Coffee

Beretta, M. J. G.; Harakava, Ricardo; Chagas, C. M.; Derrick, K. S.; Barthe, G. A.; Ceccardi, Toni L.; Lee, R. F.; Paradela, Osvaldo; Sugimori, Mauro Hideo; Ribeiro, Isadora Almeida

doi:10.1094/pd-80-0821d

Cited by 39 publications

(14 citation statements)

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“…Indeed, Xy. fastidiosa strains isolated from these two hosts are considered closely related (Beretta et al, 1996;Lima et al, 1998;Paradela-Filho et al, 1997), but in our work, the f14 primers showed a differential screening among coffee strains. Three coffee-Xy.…”

Section: Sensitivity Of Detection Of the Primers F14contrasting

confidence: 48%

“…New diseases associated with Xy. fastidiosa have recently been described in orange (Chang et al, 1993;Hartung et al, 1994) and coffee (Beretta et al, 1996;Paradela-Filho et al, 1997;Lima et al, 1998) trees in Brazil; these diseases were designated citrus variegated chlorosis (CVC) and coffee leaf scald, respectively. A rapid dissemination of CVC is occurring in the major citrus-growing areas in Brazil, with associated large economic losses.…”

Section: H F E K R E I K a A N D O T H E R S ~-mentioning

confidence: 99%

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A simplified subtractive hybridization protocol used to isolate DNA sequences specific to Xylella fastidiosa

et al. 1999

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showing no hybridization to the driver DNA, was used as a probe specific to Xy. fastidiosa from citrus and oleander. This fragment was sequenced and the predicted protein showed 40% similarity to the central region of flagellin of Escherichia co/i serotypes H1 and H12. A pair of internal primers (f14-1 and f14-2) was designed for amplification of Xy. fastidiosa DNA. These primers detected Xy. fastidiosa strains isolated from citrus and oleander and yielded an amplification product of about 600 bp. They were also able to detect the bacteria in extracts from citrus plants with or without symptoms of disease. No amplification reaction was obtained using DNA extracted from other species and pathovars of Xanthomonas, Pseudomonas cichorii, Ennrinia carotovora, Agrobacterium tumefaciens and phytopathogens of citrus (Xanthomonas axonopodis pv. citri) and coffee (Burkholderia andropogonis, P. cichorii, Pseudomonas syringae pv. garcae). The isolation of a DNA fragment specific to Xy. fastidiosa from citrus showed that the simplified protocol of subtractive hybridization used in this work is potentially applicable to other microorganisms. I CTIODi

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Section: Sensitivity Of Detection Of the Primers F14contrasting

confidence: 48%

Section: H F E K R E I K a A N D O T H E R S ~-mentioning

confidence: 99%

A simplified subtractive hybridization protocol used to isolate DNA sequences specific to Xylella fastidiosa

et al. 1999

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“…pauca was originally unable to infect coffee plants, but that adaptation to this host plant became possible only after the introduction of novel genetic variation. The first description of CLS appears in 1995 (21). Even if the disease had been previously present but unidentified, it has been hypothesized that the recombination event leading to the host shift has been a relatively recent event (16).…”

Section: Discussionmentioning

confidence: 99%

“…Coffee leaf scorch (CLS), due to X. fastidiosa, was first identified in 1995 in Brazil (21) and later in Latin American coffeeproducing countries such as Costa Rica (22). Symptoms of CLS include drying of infected branches, shortening of internode regions, decreased fruit size, chlorosis, and early senescence of leaves.…”

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confidence: 99%

New Coffee Plant-Infecting Xylella fastidiosa Variants Derived via Homologous Recombination

Jacques

Denancé

Legendre³

et al. 2016

Appl Environ Microbiol

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Xylella fastidiosa is a xylem-limited phytopathogenic bacterium endemic to the Americas that has recently emerged in Asia and Europe. Although this bacterium is classified as a quarantine organism in the European Union, importation of plant material from contaminated areas and latent infection in asymptomatic plants have engendered its inevitable introduction. In 2012, four coffee plants (Coffea arabica and Coffea canephora) with leaf scorch symptoms growing in a confined greenhouse were detected and intercepted in France. After identification of the causal agent, this outbreak was eradicated. Three X. fastidiosa strains were isolated from these plants, confirming a preliminary identification based on immunology. The strains were characterized by multiplex PCR and by multilocus sequence analysis/typing (MLSA-MLST) based on seven housekeeping genes. One strain, CFBP 8073, isolated from C. canephora imported from Mexico, was assigned to X. fastidiosa subsp. fastidiosa/X. fastidiosa subsp. sandyi. This strain harbors a novel sequence type (ST) with novel alleles at two loci. The two other strains, CFBP 8072 and CFBP 8074, isolated from Coffea arabica imported from Ecuador, were allocated to X. fastidiosa subsp. pauca. These two strains shared a novel ST with novel alleles at two loci. These MLST profiles showed evidence of recombination events. We provide genome sequences for CFBP 8072 and CFBP 8073 strains. Comparative genomic analyses of these two genome sequences with publicly available X. fastidiosa genomes, including the Italian strain CoDiRO, confirmed these phylogenetic positions and provided candidate alleles for coffee plant adaptation. This study demonstrates the global diversity of X. fastidiosa and highlights the diversity of strains isolated from coffee plants.

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“…pauca CVC strain (9a5c), the first plant pathogenic bacterium to have its genome completely sequenced and annotated (44). It also infects coffee, another economically important plant in Brazil: coffee leaf scorch (CLS) was first documented in Brazil in 1995 (3). X. fastidiosa subsp.…”

mentioning

confidence: 99%

Detecting Genetic Introgression: High Levels of Intersubspecific Recombination Found in Xylella fastidiosa in Brazil

Nunney

Yuan

Bromley

et al. 2012

Appl Environ Microbiol

129

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b Documenting the role of novel mutation versus homologous recombination in bacterial evolution, and especially in the invasion of new hosts, is central to understanding the long-term dynamics of pathogenic bacteria. We used multilocus sequence typing (MLST) to study this issue in Xylella fastidiosa subsp. pauca from Brazil, a bacterium causing citrus variegated chlorosis (CVC) and coffee leaf scorch (CLS). All 55 citrus isolates typed (plus one coffee isolate) defined three similar sequence types (STs) dominated by ST11 (85%), while the remaining 22 coffee isolates defined two STs, mainly ST16 (74%). This low level of variation masked unusually large allelic differences (>1% divergence with no intermediates) at five loci (leuA, petC, malF, cysG, and holC). We developed an introgression test to detect whether these large differences were due to introgression via homologous recombination from another X. fastidiosa subspecies. Using additional sequencing around these loci, we established that the seven randomly chosen MLST targets contained seven regions of introgression totaling 2,172 bp of 4,161 bp (52%), only 409 bp (10%) of which were detected by other recombination tests. This high level of introgression suggests the hypothesis that X. fastidiosa subsp. pauca became pathogenic on citrus and coffee (crops cultivated in Brazil for several hundred years) only recently after it gained genetic variation via intersubspecific recombination, facilitating a switch from native hosts. A candidate donor is the subspecies infecting plum in the region since 1935 (possibly X. fastidiosa subsp. multiplex). This hypothesis predicts that nonrecombinant native X. fastidiosa subsp. pauca (not yet isolated) does not cause disease in citrus or coffee.

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First Report ofXylella fastidiosain Coffee

Cited by 39 publications

References 0 publications

A simplified subtractive hybridization protocol used to isolate DNA sequences specific to Xylella fastidiosa

A simplified subtractive hybridization protocol used to isolate DNA sequences specific to Xylella fastidiosa

New Coffee Plant-Infecting Xylella fastidiosa Variants Derived via Homologous Recombination

Detecting Genetic Introgression: High Levels of Intersubspecific Recombination Found in Xylella fastidiosa in Brazil

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