First Report of <i>Tomato severe rugose virus</i>, a Tomato-Infecting Begomovirus, in Soybean Plants in Brazil

Macedo, M. A.; Barreto, Sarah S.; Costa, Tairine Melo; Rocha, Geisiane Alves; Dianese, Érico de Campos; Gilbertson, R. L.; Inoue‐Nagata, Alice K.

doi:10.1094/pdis-05-17-0644-pdn

Cited by 19 publications

(14 citation statements)

References 5 publications

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“…These results support the hypothesis that soybean plants may play an important role as an amplifier host of ToSRV for tomato crops in the field, although this does not seem to be a frequent phenomenon. Successful amplification will depend on several factors, including: Macedo et al (2017b). However, plants of the same cultivar were not infected with the ToSRV isolate used in the present study.…”

Section: Discussionmentioning

confidence: 96%

“…The amplifier-host hypothesis (Figure 1) helps to explain the recurrent rapid epidemics that occur in Brazilian conditions, despite both the weak reservoir force of infection and the prevention of secondary spread by insecticide sprays for vector control. The insight to include amplifier hosts in the conceptual model of ToSRV/tomato epidemics was based on two recent surveys carried out in central Brazil, one reporting that 2.9% of asymptomatic common bean plants were infected with ToSRV (Macedo et al, 2017a), and the other reporting that 3.3% of asymptomatic soybean plants were infected with the same begomovirus (Macedo et al, 2017b). Furthermore, data collected in 2018 in the Sumaré region (state of São Paulo) in a senescent soybean crop near a recently transplanted tomato crop (sprayed with insecticide three times per week) showed a > 10% incidence of asymptomatic ToSRV-infected soybean plants and a 57-70% incidence of symptomatic ToSRV-infected tomato plants.…”

Section: Reservoir and Amplifier Hosts In Botanical Epidemiologymentioning

confidence: 99%

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Amplifier Hosts May Play an Essential Role in Tomato Begomovirus Epidemics in Brazil

et al. 2020

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Current control of tomato golden mosaic disease, caused in Brazil predominantly by tomato severe rugose virus (ToSRV), is dependent on both, planting resistant/tolerant hybrids and intensive insecticide sprays (two to three per week) for controlling Bemisia tabaci, the vector of ToSRV. Resistant hybrids only confer moderate resistance to infection by ToSRV and some tolerance to the disease. Insecticide sprays, although widely used, have failed in most tomato production areas in Brazil, as they are unable to reduce primary spread, i.e., infection caused by the influx of viruliferous whiteflies coming from external sources of inoculum. Severe epidemics are recurrently observed in some tomato fields in several Brazilian regions, which prompted us to postulate the existence in the agroecosystem, in some places and time, of amplifier hosts that provide the necessary force of infection for epidemics to occur, even in the absence of secondary spread in the target crop. Amplifier hosts are ideally asymptomatic, occur in high density near the target crop, and support growth of both virus and vector. Soybean and common bean are potential amplifier hosts for begomovirus in tomato crops. Our results support the hypothesis that soybean plants may play an important role as an amplifier host of ToSRV for tomato crops in the field, although this does not seem to be a frequent phenomenon. Successful amplification will depend on several factors, including the soybean cultivar, the soybean stage of development at the moment of infection, the ToSRV isolate, and the perfect synchrony between the beginning of a soybean field and the end of a ToSRV-infected crop, and, later, between the senescence of the ToSRVinfected soybean plants and the new tomato crop. The concept of amplifier hosts has been widely used in ecology of zoonoses but, to our knowledge, has never been used in botanical epidemiology.

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Section: Discussionmentioning

confidence: 96%

Section: Reservoir and Amplifier Hosts In Botanical Epidemiologymentioning

confidence: 99%

Amplifier Hosts May Play an Essential Role in Tomato Begomovirus Epidemics in Brazil

et al. 2020

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“…Begomovirus infecting pepper (chilli) and tomato plants have narrow host ranges and were shown to infect plants of other families, e.g., Fabaceae [common bean and soybean (Glycine max)] and Cucurbitaceae (20,21).…”

Section: Introductionmentioning

confidence: 99%

Detection of Begomovirus in Chilli and Tomato Plants Using Functionalized Gold Nanoparticles Authors: Lavanya. R and Arun. V

Ramesh

Viswanathan

2021

Preprint

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Begomoviruses are a major class of Geminiviruses that affects most dicotyledonous plants and causes heavy economic losses to farmers. Early detection of begomovirus is essential to control the spread of the disease and prevent loss. Many available detection methods like ELISA, immunosorbent electron microscopy, PCR or qPCR require expertise in handling sophisticated instruments, complex data interpretation and costlier chemicals, enzymes or antibodies. Hence there is a need for a simpler detection method, here we report the development of a visual detection method based on functionalized gold nanoparticles (AuNP probe assay). The assay was able to detect upto 500 ag/𝜇l of begomoviral DNA (pTZCCPp3, a clone carrying partial coat protein gene) suspended in MilliQ water. Screening of chilli plants for begomoviral infection by PCR (Deng primers) and AuNP assay showed that AuNP assay (77.7%) was better than PCR (49.4%). The AuNP assay with clccpi1 probe was able to detect begomoviral infection in chilli, tomato, common bean, green gram and black gram plants which proved the utility and versatility of the AuNP assay. The specificity of the assay was demonstrated by testing with total DNA from different plants that are not affected by begomoviruses.

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“…Additionally, this virus has also been found naturally infecting potatoes ( Solanum tuberosum ), which develop yellow mosaic symptoms; peppers ( Capsicum spp. ), which develop symptoms of chlorotic spots, rugosity, and mild leaf distortion; and soybeans and common beans, in which the virus is symptomless (Inoue‐Nagata et al , ; Macedo et al , , ). Moreover, several weeds are known hosts of ToSRV, such as Crotalaria sp., Datura stramonium , Euphorbia heterophylla , Sida spp., Nicandra physaloides , and Malva spp., developing symptoms of yellow mosaic, leaf distortion, and stunting (Barbosa et al , ; Barreto et al , ; Macedo et al , ).…”

Section: Introductionmentioning

confidence: 99%

“…However, the main strategy used today is based on vector control with insecticides (Gilbertson et al, 2011;Lapidot et al, 2014). The Brazilian state of Goiás has implemented a tomato-free period for managing B. tabaci and ToSRV spread, but this policy has not been efficient due to the capacity of ToSRV to infect other vegetable and weed species (Inoue-Nagata et al, 2016b;Macedo et al, 2017aMacedo et al, , 2017b.…”

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confidence: 99%

Evaluation of Datura stramonium and Nicandra physaloides as reservoirs of tomato severe rugose virus and whiteflies

et al. 2020

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Tomato severe rugose virus (ToSRV) is the most important begomovirus transmitted and spread by the whitefly Bemisia tabaci in tomato crops in Brazil. Cultural practices are being adopted, along with insecticides, for controlling this virus. However, little is known about the importance of weeds in the pathosystem, which can contribute to the failure of these practices. This work aimed to evaluate the role of Datura stramonium and Nicandra physaloides as alternative hosts of ToSRV and verify the viral influence on the biological performance of Bemisia tabaci Middle East‐Asia Minor 1 (MEAM1) and Mediterranean (MED) cryptic species. N. physaloides was a better alternative host for ToSRV when combined with MEAM1 whiteflies, while D. stramonium was mostly a good host for whitefly reproduction. Viral infection improved MEAM1 performance on both host plants but affected MED performance negatively. These data suggest that both weeds can be of some importance for the pathosystem, and their control should be included in management programmes.

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First Report of Tomato severe rugose virus, a Tomato-Infecting Begomovirus, in Soybean Plants in Brazil

Cited by 19 publications

References 5 publications

Amplifier Hosts May Play an Essential Role in Tomato Begomovirus Epidemics in Brazil

Amplifier Hosts May Play an Essential Role in Tomato Begomovirus Epidemics in Brazil

Detection of Begomovirus in Chilli and Tomato Plants Using Functionalized Gold Nanoparticles Authors: Lavanya. R and Arun. V

Evaluation of Datura stramonium and Nicandra physaloides as reservoirs of tomato severe rugose virus and whiteflies

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