First report of <i>Tomato leaf curl New Delhi virus</i> infecting courgette in France

Desbiez, Cécile; Gentit, Pascal; Cousseau-Suhard, Pascaline; Renaudin, I.; Verdin, Eric

doi:10.1002/ndr2.12006

Cited by 7 publications

(5 citation statements)

References 5 publications

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“…In Morocco, similar symptoms were observed in 2017, on zucchini crops grown in Agadir and Taroudant regions (Radouane et al, 2018). The virus was also reported in southern France in September 2020 on zucchini (EPPO, 2020;Desbiez et al, 2021). These reports suggest recent ToLCNDV introductions into North Africa and Southern Europe.…”

Section: Begomovirusessupporting

confidence: 57%

Viruses of cucurbit crops: current status in the Mediterranean Region

Radouane

Ezrari

Belabess

et al. 2021

Phytopathol. Mediterr.

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Cucurbits are among the most cultivated crops, and the most economically important species are melon (Cucumis melo L.), cucumber (Cucumis sativus L.), watermelon (Citrullus lanatus Thumb.), squash (Cucurbita pepo L.), and pumpkin (Cucurbita spp.). These crops have become important income sources providing export and local consumption commodities in many Mediterranean countries. Increased area of cucurbits has led to the emergence of several viral diseases, which can have impacts on crop production and threaten agricultural sustainability. An overview of the most damaging cucurbit viruses in the Mediterranean area is provided to improve understanding of the diseases they cause and to emphasize effective disease management strategies. An updating of the geographical distribution of these viruses, the symptoms they cause and their means of transmission is also provided. Disease management methods and measures by farmers and phytosanitary authorities to address the virus outbreaks are outlined, including diagnostics, use of tolerant cultivars, and chemical and biological vector control. Mediterranean region farmers have learned many lessons from the damaging pandemics caused by cucurbit viruses, through the extensive published research, and this review provides a basis for managing future outbreaks of newly emerging virus infections.

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Section: Begomovirusessupporting

confidence: 57%

Viruses of cucurbit crops: current status in the Mediterranean Region

Radouane

Ezrari

Belabess

et al. 2021

Phytopathol. Mediterr.

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“…Afterwards, it was detected in other countries on the Indian subcontinent (Bangladesh and Pakistan), in Southeast Asia (Philippines, Indonesia, Sri Lanka, Thailand, and Taiwan), and the Middle East (Iran) [ 7 ]. In the Mediterranean basin, ToLCNDV was detected in Spain, Italy, Tunisia, Morocco, Algeria, Greece, and France [ 8 , 9 , 10 , 11 , 12 , 13 , 14 ]. Regarding Italy, in October 2015, it was detected for the first time in the horticultural area of Trapani province (Sicily), where severe symptoms on zucchini squash in the open field were observed, not previously reported by growers [ 9 ]; subsequently, it was also found in Sardinia [ 15 ], Lazio [ 16 ], Calabria, Campania, and Apulia regions [ 2 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Development of an In-Field Real-Time LAMP Assay for Rapid Detection of Tomato Leaf Curl New Delhi Virus

Caruso

Ragona

Bertacca

et al. 2023

Plants

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Tomato leaf curl New Delhi virus (ToLCNDV) represents a threat to economically important horticultural crops. A real-time loop-mediated isothermal amplification (LAMP) assay for in-field ToLCNDV detection was developed, coupled to a rapid sample preparation method, and tested both in field and laboratory conditions on zucchini squash, tomato, and pepper samples. A set of six LAMP primers was designed for specific ToCLNDV detection, targeting a 218-nucleotide sequence within the AV1 gene. The sensitivity, specificity and accuracy of the real-time LAMP assay and comparison with canonical PCR were evaluated. The real-time LAMP assay developed was about one-thousand times more sensitive than the conventional PCR method, detecting a total of 4.41 × 102 genome copies as minimum target; no cross-reactivity was detected with the other geminiviruses used as the outgroup. The rapid sample preparation method allows for a reliable detection with a low reaction delay (≈2–3 min) compared to canonical DNA extraction, providing results in less than 45 min. Lastly, an increase in ToLCNDV-positive sample detection was observed compared to PCR, in particular for asymptomatic plants (85% and 71.6%, respectively). The real-time LAMP assay developed is a rapid, simple, specific, and sensitive technique for ToLCNDV detection, and it can be adopted as a routine test, for both in-field and laboratory conditions.

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“…Resistance to the geminivirus ToLCNDV has been investigated in cucurbits (Saez et al, 2021) since the virus is a major threat to these crops (Desbiez et al, 2021;Fortes et al, 2016;Juarez et al, 2014;Panno et al, 2016;Orfanidou et al, 2019;Zaidi et al, 2017). In melon (Cucumis melo) ToLCNDV resistance is attributed to two minor QTLs in chromosomes 2 and 12 and one major recessive QTL in chromosome 11: 30,112,563-30,725,907 nt (melonomics v.4) (Sa ́ez et al, 2017;Roman et al, 2019;Romay et al, 2019;Saez et al, 2022) whereas in Cucurbita moschata the same type of resistance was mapped in a major recessive QTL on chromosome 8 (Saez et al, 2016;Sa ́ez et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

DNA primase large subunit is an essential plant gene for geminiviruses, putatively priming viral ss-DNA replication

Siskos

Antoniou

Riado³

et al. 2023

Front. Plant Sci.

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The family of Geminiviridae consists of more than 500 circular single-stranded (ss) DNA viral species that can infect numerous dicot and monocot plants. Geminiviruses replicate their genome in the nucleus of a plant cell, taking advantage of the host’s DNA replication machinery. For converting their DNA into double-stranded DNA, and subsequent replication, these viruses rely on host DNA polymerases. However, the priming of the very first step of this process, i.e. the conversion of incoming circular ssDNA into a dsDNA molecule, has remained elusive for almost 30 years. In this study, sequencing of melon (Cucumis melo) accession K18 carrying the Tomato leaf curl New Delhi virus (ToLCNDV) recessive resistance quantitative trait locus (QTL) in chromosome 11, and analyses of DNA sequence data from 100 melon genomes, showed a conservation of a shared mutation in the DNA Primase Large subunit (PRiL) of all accessions that exhibited resistance upon a challenge with ToLCNDV. Silencing of (native) Nicotiana benthamiana PriL and subsequent challenging with three different geminiviruses showed a severe reduction in titers of all three viruses, altogether emphasizing an important role of PRiL in geminiviral replication. A model is presented explaining the role of PriL during initiation of geminiviral DNA replication, i.e. as a regulatory subunit of primase that generates an RNA primer at the onset of DNA replication in analogy to DNA Primase-mediated initiation of DNA replication in all living organisms.

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First report of Tomato leaf curl New Delhi virus infecting courgette in France

Cited by 7 publications

References 5 publications

Viruses of cucurbit crops: current status in the Mediterranean Region

Viruses of cucurbit crops: current status in the Mediterranean Region

Development of an In-Field Real-Time LAMP Assay for Rapid Detection of Tomato Leaf Curl New Delhi Virus

DNA primase large subunit is an essential plant gene for geminiviruses, putatively priming viral ss-DNA replication

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