First report of<i>Squash leaf curl china virus</i>causing mosaic symptoms on summer squash (<i>Cucurbita pepo</i>) grown in Varanasi district of India

Saritha, R. K.; Bag, T. K.; Loganathan, M.; Rai, Anurag; Rai, Mathura

doi:10.1080/03235400902952301

Cited by 9 publications

(6 citation statements)

References 7 publications

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“…The SLCCNV caused symptoms of leaf curling and blistering on wax gourd, symptoms of leaf curling and blistering on oriental melon, leaf curling, blistering and yellow spots on pumpkin and squash. Those symptoms are similar to what was previously described [13,14,16,18]. The SLCCNV-infected bottle gourd exhibited symptoms of leaf curling and yellowing, which are similar to those described previously as of chlorotic mottling, mild curling and serious stunting [15].…”

Section: Discussionsupporting

confidence: 89%

“…ToLCNDV have been recorded in India [29], Southeast Asia [3], Spain [30], the Mediterranean [31] and Taiwan [32]. SLCCNV is currently distributed in China [33], India [14][15][16][17] and Southeast Asia [12,13,34]. The SLCuPV was identified in the Philippines and Taiwan [5,19].…”

Section: Discussionmentioning

confidence: 99%

“…So far, ToLCNDV was identified on more than 15 cucurbit crops distributed in nine countries [10]. SLCCNV was identified on more than six cucurbit crops in eight countries [12][13][14][15][16][17][18]. The SLCuPV was first identified in the Philippines and most closely related to the SLCCNV (88% nucleotide identity of DNA-A) [5].…”

Section: Introductionmentioning

confidence: 99%

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Genetic Diversity, Pathogenicity and Pseudorecombination of Cucurbit-Infecting Begomoviruses in Malaysia

Chen

Lai

Lin

et al. 2021

Plants

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Cucurbits are important crops in the world. However, leaf curl disease constrains their production. Here, begomovirus diversity and pathogenicity associated with the disease in Malaysia were studied based on 49 begomovirus-detected out of 69 symptomatic plants from seven cucurbit crops in 15 locations during 2016 and 2017. The presence of Squash leaf curl China virus (SLCCNV) and Tomato leaf curl New Delhi virus (ToLCNDV) were confirmed by virus detection by polymerase chain reaction, viral DNA sequence analysis and specific detection of the viral components. ToLCNDV Malaysian isolates were further distinguished into strains A, B, C and D. Virus co-infection was detected in bitter gourd, bottle gourd and squash. Among them, eight bitter gourd samples were detected without SLCCNV DNA-A. However, one bottle gourd and five squash samples were without ToLCNDV DNA-B. Pseudorecombination of ToLCNDV DNA-A and SLCCNV DNA-B was detected in two bitter gourd samples. The pathogenic viruses and pseudorecombinants were confirmed by agroinoculation. The viral DNA-B influencing on symptomology and host range was also confirmed. The results strengthen the epidemic of cucurbit-infecting begomovirus in Malaysia as well as Southeast Asia. Especially, the natural pseudorecombinant of begomovirus that extends host range and causes severe symptom implies a threat to crops.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

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Genetic Diversity, Pathogenicity and Pseudorecombination of Cucurbit-Infecting Begomoviruses in Malaysia

Chen

Lai

Lin

et al. 2021

Plants

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“…SLCCV has been reported from different cucurbit hosts. SLCCV infecting squash (Cucurbita pepo) reduced production in India and Pakistan and resulted in huge economic losses (Saritha et al, 2011;Tahir et al, 2010b). SLCCV have also been reported from cucurbits like pumpkin (Vo et al, 2022), ash gourd (Riyaz et al, 2015) and melon (Wu et al, 2020).…”

Section: Resultsmentioning

confidence: 99%

Investigation on disease incidence, distribution and diversity of Begomoviruses infecting cucurbitaceous crops in North Bengal, India

Tamang,

Saha,

Saha

et al. 2024

JEB

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Aim: To study the disease incidence and diversity of Begomovirus infection and crop loss due to spread of virus across different districts of North Bengal. Methodology: Several fields in different districts of North Bengal were surveyed and potential begomoviral-infected samples were collected for further analysis. Total DNA extraction, PCR analysis, sequencing and phylogenetic analysis were conducted. Results: During 2019-2021, ten fields of each district were surveyed and begomoviral occurrence was observed in almost every field. The disease incidence in different districts varied from 15-45%. The highest disease (42.5%) incidence of was observed in the Darjeeling district while the lowest (15%) was recorded from Alipurduar district. The maximum crop loss due to Begomovirus was 64-100% in bitter gourd, 40-80% in cucumber and 20-30% in wax gourd. 153 samples from 6 different cucurbits were collected and analysed. PCR using AV494/AC1048 primers that amplified coat protein region (~550bp) showed 74% of the samples as positive. Upon sequencing and BLAST analysis, ToLCNDV, SLCCV and BGYVV were detected. In phylogenetic analysis, the isolates clustered in three separate clades based on Begomovirus species irrespective of the hosts. Present isolates showed more closeness to Indian and Bangladesh isolates. Interpretation: The documentation of disease severity caused by Begomovirus is of extreme importance for food security of the people. Present study revealed that Begomovirus is present in all the crop field studied. The results indicated an urgent need for the management of the viruses to save crops. Key words: Begomovirus, Cucurbitaceous crops, Disease incidence, North Bengal

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“…Begomoviruses possess either monopartite genome DNA A or bipartite genomes DNA A and B (Asigul et al, 2018). Squash leaf curl China virus (SLCCNV) belongs to the genus Begomovirus and is an important bipartite virus that causes severe economic losses in Cucurbitaceae crops such as wax gourd, squash, and pumpkin (Saritha et al, 2011;Riyaz et al, 2013;Maina et al, 2017). The disease incidence in ash gourds can reach 100% in the field (Riyaz et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

AC5 protein encoded by squash leaf curl China virus is an RNA silencing suppressor and a virulence determinant

Liu

Kang

et al. 2022

Front. Microbiol.

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Squash leaf curl China virus (SLCCNV) is a bipartite Begomovirus. The function of the protein AC5, which is encoded by SLCCNV, is unknown. Here, we confirmed that the 172-amino acids (aa) long AC5 protein of SLCCNV could suppress single-stranded RNA but not double-stranded RNA-induced post-transcriptional gene silencing (PTGS). Furthermore, we determined that the C-terminal domain (96–172 aa) of the AC5 protein was responsible for RNA silencing suppressor (RSS) activity via deletion mutant analysis. The AC5 protein can reverse GFP silencing and inhibit systemic silencing of GFP by interfering with the systemic spread of the GFP silencing signal. The SLCCNV AC5 protein was localized to both the nucleus and cytoplasm of Nicotiana benthamiana cells. Furthermore, deletion analysis showed that the putative nuclear localization signal (NLS, 102–155 aa) was crucial for the RNA silencing suppression activity of AC5. In addition, the AC5 protein elicited a hypersensitive response and enhanced potoao virus X (PVX) RNA accumulation in infected N. benthamiana plants. Using the infectious clones of the SLCCNV and SLCCNV-AC5 null mutants, mutational analysis confirmed that knockout of the AC5 gene abolished SLCCNV-induced leaf curl symptoms, showing SLCCNV AC5 is also a virulence determinant.

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First report ofSquash leaf curl china viruscausing mosaic symptoms on summer squash (Cucurbita pepo) grown in Varanasi district of India

Cited by 9 publications

References 7 publications

Genetic Diversity, Pathogenicity and Pseudorecombination of Cucurbit-Infecting Begomoviruses in Malaysia

Genetic Diversity, Pathogenicity and Pseudorecombination of Cucurbit-Infecting Begomoviruses in Malaysia

Investigation on disease incidence, distribution and diversity of Begomoviruses infecting cucurbitaceous crops in North Bengal, India

AC5 protein encoded by squash leaf curl China virus is an RNA silencing suppressor and a virulence determinant

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