First Report of Strawberry latent ringspot virus in Vaccinium darrowii

Abstract

Strawberry latent ringspot virus (SLRSV) was detected in blueberry (Vaccinium darrowii), a host not previously reported. A total of 89 samples were obtained from one site in the North Island of New Zealand and tested by reverse transcription—polymerase chain reaction (RT‐PCR) assay. Both RT‐PCR and sequencing results showed the presence of SLRSV in four samples. Although SLRSV is known to have a wide host range, this appears to be the first report of SLRSV in blueberry.

Known and New Emerging Viruses Infecting Blueberry

“…Strawberry latent ringspot virus (SLRSV) had not been found in blueberry until recently in New Zealand in 2014, where it was discovered in V. darrowii, a new host of SLRSV [26]. SLRSV was previously discovered to naturally infect wild and cultivated rosaceous plants (e.g., black currant, cherry, plum, raspberry, and strawberry) in Scotland in 1964 [81], and has since been recorded in other continents around the world, including Asia, Oceania, and North America, in addition to European countries [82,83].…”

“…After being formerly assigned in the genus Nepovirus and Sadwavirus, SLRSV is currently placed in an unassigned genus of the family Secoviridae [71,84,85]. The transmission mode of SLRV in blueberry has yet to be determined, while mechanical inoculation of the virus into herbaceous hosts were not successful in previous study [26]. However, SLRSV is known to be transmitted by the nematodes X. diversicaudatum and X. coxi, and is seedborne in some plant species such as raspberry and celery [82].…”

Creation of a new genus in the family Secoviridae substantiated by sequence variation of newly identified strawberry latent ringspot virus isolates

“…The online version of this article (https ://doi.org/10.1007/s0070 5-019-04437 -0) contains supplementary material, which is available to authorized users. 1 3 from new hosts and geographic regions, revealed considerable diversity among isolates of this widespread virus [2,12,15,19,26].…”

“…Moreover, the reverse transcription polymerase chain reaction (RT-PCR) test described by Tang et al [19] failed to detect some isolates that tested positive in DAS-ELISA. This lack of concordance is most likely due to the diversity of the SLRSV isolates tested [26]. To gain more insight in the diversity of SLRSV sequences, high-throughput sequencing (HTS) was performed on eleven SLRSV isolates from different virus collections and also on a selection of isolates from diagnostic lily samples that reacted differently in serological and molecular tests.…”