First Report of <i>Plum bark necrosis stem pitting-associated virus</i> Infecting Flowering Cherry in Japan

Candresse, Thierry; Faure, Chantal; Theil, Sébastien; Marais, Armelle

doi:10.1094/pdis-09-16-1324-pdn

Cited by 10 publications

(6 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Of the viruses infecting plum trees, PBNSPaV was only identified in the EP plum cultivar (P. domestica), not the other P. salicina cultivars or P. cerasifera. By contrast, infection of PBNSPaV in P. cerasifera and P. serrulata has been previously reported in China (Cui et al, 2011) and Japan (Candresse et al, 2017). Moreover, a previous study identified two PBNSPaV isolates from P. salicina in China by the FLX454 system (Marais et al, 2014).…”

Section: Discussionmentioning

confidence: 91%

Identification of viruses infecting six plum cultivars in Korea by RNA-sequencing

Choi

Lian

et al. 2020

PeerJ

View full text Add to dashboard Cite

Background Plums are a kind of stone fruit, a category that includes peaches, cherries, apricots, and almonds. In Korea, Japanese plum trees are usually cultivated as they best suit the climate. To date, there have been few studies in Korea on viruses infecting plum trees compared to those infecting peach trees. Methods To identify viruses and viroids infecting plum trees, we collected leaf samples from six different plum cultivars and subjected them to RNA-sequencing (RNA-seq). Six different plum transcriptomes were de novo assembled using the Trinity assembler followed by BLAST searching against a viral reference database. Results We identified hop stunt viroid (HSVd) and six viruses, including apple chlorotic leaf spot virus (ACLSV), little cherry virus-1 (LChV-1), peach virus D (PeVD), peach leaf pitting-associated virus (PLPaV), plum bark necrosis stem pitting-associated virus (PBNSPaV), and prunus necrotic ringspot virus (PNRSV), from six plum cultivars by RNA-seq. RT-PCR confirmed the infection of HSVd and three viruses—ACLSV, PBNSPaV, and PNRSV—in plum trees. However, RT-PCR demonstrated that plum trees in this study were not infected by LChV-1, PeVD, or PLPaV. It is likely that the three viruses LChV-1, PeVD, and PLPaV as identified by RNA-seq were contaminants from other peach libraries caused by index misassignment, which suggests that careful confirmation by other methods should be carried out in next-generation sequencing (NGS)-based virus identification. Taken together, we identified a viroid and three viruses infecting plum trees in Korea.

show abstract

Section: Discussionmentioning

confidence: 91%

Identification of viruses infecting six plum cultivars in Korea by RNA-sequencing

Choi

Lian

et al. 2020

PeerJ

View full text Add to dashboard Cite

show abstract

“…in Dinuba, CA, U.S.A. [ 10 ]. Since then PBNSPaV has been shown to be worldwide distributed, affecting a broad range of cultivated and ornamental Prunus species and causes decline, gummosis, flattening of scaffold branches, and stem necrotic pits in some diseased trees [ 9 ]. Up to now, there have been no reports of PBNSPaV infecting grape in China.…”

Section: Discussionmentioning

confidence: 99%

“…1 ). PBNSPaV is a viral species that belongs to the genus Ampelovirus , family Closteroviridae and has been shown to be worldwide distributed, affecting a broad range of Prunus species [ 5 , 8 , 9 ]. To our knowledge, this is the first report of PBNSPaV infecting grapevine in China.…”

Section: Introductionmentioning

confidence: 99%

First report of Plum bark necrosis stem pitting-associated virus infecting grapevine in China

Yang

Sun

et al. 2020

Virol J

View full text Add to dashboard Cite

Background Virus disease is one of the main diseases in grapevine, and there has been no report on Plum bark necrosis and stem pitting-associated virus infecting grapevine in China. Objective The leaf samples of grapevine cultivar 'Cabernet Gernischt' were collected from Shandong province, which the leaves suffered from viral-like symptoms with spotting and crinkle. Methods Small RNA-seq combined with reverse transcription PCR (RT-PCR) were performed to detect the potential viruses in these field samples. Phylogenetic tree was constructed using the neighbor joining method in MEGA 5.1 Conclusions This is the first report of PBNSPaV infecting grapevine in China, contributing to a better understanding of the epidemiology and host range distribution of this pathogen.

show abstract

“…1). PBNSPaV is a viral species that belongs to the genus Ampelovirus, family Closteroviridae and has been shown to be worldwide distributed, affecting a broad range of Prunus species [5][6][7]. To our knowledge, this is the rst report of PBNSPaV infecting grapevine in China.…”

Section: Main Textmentioning

confidence: 99%

First Report of Plum bark necrosis stem pitting-associated virus Infecting Grapevine in China

Yang

Sun

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Virus disease is one of the main diseases in grapevine, and there has been no report on Plum bark necrosis and stem pitting-associated virus infecting grapevine in China.Objective: The leaf samples of grapevine cultivar 'Cabernet Gernischt' were collected from Shandong province, which the leaves suffered from viral-like symptoms with spotting and crinkle.Methods: Small RNA-seq combined with reverse transcription PCR (RT-PCR) were performed to detect the potential viruses in these field samples. Phylogenetic tree was constructed using the neighbor joining method in MEGA 5.1Conclusions: This is the first report of PBNSPaV infecting grapevine in China, contributing to a better understanding of the epidemiology and host range distribution of this pathogen.

show abstract

First Report of Plum bark necrosis stem pitting-associated virus Infecting Flowering Cherry in Japan

Cited by 10 publications

References 3 publications

Identification of viruses infecting six plum cultivars in Korea by RNA-sequencing

Identification of viruses infecting six plum cultivars in Korea by RNA-sequencing

First report of Plum bark necrosis stem pitting-associated virus infecting grapevine in China

First Report of Plum bark necrosis stem pitting-associated virus Infecting Grapevine in China

Contact Info

Product

Resources

About