First Report of <i>Phytophthora cinnamomi</i> Causing Root and Stem Rot of Blueberry (<i>Vaccinium corymbosum</i>) in China

Lan, Chengzhong; Ruan, Hongchun; Yao, Jinai

doi:10.1094/pdis-03-16-0345-pdn

Cited by 8 publications

(5 citation statements)

References 5 publications

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“…P. cinnamomi has led to significant declines of the agricultural livestock ecosystem in the Iberian Peninsula ( Rodriguez-Romero et al., 2021 ) and the production of highbush blueberry in Germany ( Nechwatal and Jung, 2021 ) and also led to root and stem rot in Vaccinium corymbosum L. ( Lan et al., 2016a ) and Castanea mollissima (Chinese Chestnut) ( Lan et al., 2016b ). In addition, P. cinnamomi has had severe impacts on kiwifruit production in China ( Bi et al., 2019 ) and has seriously affected the growth and yield of Carya cathayensis in Linan County, China ( Tong et al., 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Rapid detection of Phytophthora cinnamomi based on a new target gene Pcinn13739

Chen

Jiao

Zhou

et al. 2022

Front. Cell. Infect. Microbiol.

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Phytophthora cinnamomi causes crown and root wilting in more than 5,000 plant species and represents a significant threat to the health of natural ecosystems and horticultural crops. The early and accurate detection of P. cinnamomi is a fundamental step in disease prevention and appropriate management. In this study, based on public genomic sequence data and bioinformatic analysis of several Phytophthora, Phytopythium, and Pythium species, we have identified a new target gene, Pcinn13739; this allowed us to establish a recombinase polymerase amplification–lateral flow dipstick (RPA-LFD) assay for the detection of P. cinnamomi. Pcinn13739-RPA-LFD assay was highly specific to P. cinnamomi. Test results for 12 isolates of P. cinnamomi were positive, but negative for 50 isolates of 25 kinds of Phytophthora species, 13 isolates of 10 kinds of Phytopythium and Pythium species, 32 isolates of 26 kinds of fungi species, and 11 isolates of two kinds of Bursaphelenchus species. By detecting as little as 10 pg.µl−1 of genomic DNA from P. cinnamomi in a 50-µl reaction, the RPA-LFD assay was 100 times more sensitive than conventional PCR assays. By using RPA-LFD assay, P. cinnamomi was also detected on artificially inoculated fruit from Malus pumila, the leaves of Rhododendron pulchrum, the roots of sterile Lupinus polyphyllus, and the artificially inoculated soil. Results in this study indicated that this sensitive, specific, and rapid RPA-LFD assay has potentially significant applications to diagnosing P. cinnamomi, especially under time- and resource-limited conditions.

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Section: Discussionmentioning

confidence: 99%

Rapid detection of Phytophthora cinnamomi based on a new target gene Pcinn13739

Chen

Jiao

Zhou

et al. 2022

Front. Cell. Infect. Microbiol.

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“…Diseases of blueberry, in particular V. corymbosum, caused by P. cinnamomi have long been reported from all over the world. First reports from the U.S. date back to the 1970s (Sterne 1982), and the disease has since been reported from South America, Asia and Europe (Tamietti 2003;Larach et al 2009;Orlikowski et al 2015;Lan et al 2016;Huarhua et al 2018). While in 2010 P. × cambivora was isolated from a young plantation of V. ovata in Germany (Jung et al 2016), so far no incidence of Phytophthora spp.…”

Section: Discussionmentioning

confidence: 99%

“…Vaccinium corymbosum is a well-known host plant of several Phytophthora species, in particular the notorious P. cinnamomi, causing severe disease and declines all over the world (Sterne 1982;Tamietti 2003;Bryla et al 2008;Larach et al 2009;Orlikowski et al 2015;Lan et al 2016;Huarhua et al 2018). Therefore, an investigation was initiated into the potential involvement of Phytophthora spp.…”

Section: Introductionmentioning

confidence: 99%

A study of Phytophthora spp. in declining highbush blueberry in Germany reveals that P. cinnamomi can thrive under Central European outdoor conditions

Nechwatal

Jung

2021

J Plant Dis Prot

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Phytophthora cinnamomi and P. cactorum were detected in soil samples from a Vaccinium corymbosum stand in southern Germany. Both species were virulent in an underbark inoculation assay and have not been reported from highbush blueberry in Germany before. While P. cactorum is a widely distributed species in nurseries and (semi)natural habitats like forests, field reports of P. cinnamomi in Germany are rare due to its sensitivity to frost. Its occurrence in an outdoor blueberry stand indicates its potential to survive Central European winter periods that tend to be increasingly mild. Phytophthora cinnamomi was also found in the substrate of young V. corymbosum nursery plants, suggesting a high degree of contamination in commercial blueberry production and demonstrating the risk of spreading exotic invasive plant pathogens via planting of infested nursery stock.

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“…It can infect a variety of ornamental plants and woody trees, causing serious losses to agricultural and forestry crop production, and has been listed as a quarantine object by many countries [35]. P. cinnamomi has been found in Fujian [36][37][38], Jiangsu and Zhejiang [38], Hainan [39], Shanxi [40], Shanghai [41] and other provinces. It poses a threat to the production of chestnut, kiwifruit, blueberry, avocado, ornamental trees and the health of natural forests [42].…”

Section: Introductionmentioning

confidence: 99%

The New Report of Root Rot on Fatsia japonica Caused by Phytophthora nicotianae in China

Zhou

Tian

et al. 2023

Forests

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As an ornamental plant, Fatsia japonica has been widely used in gardens. From April 2021 to 2022, a disease that caused the wilting and root rot of F. japonica in a large area was observed, which eventually led to the plants wilting and dying, while the leaves did not fall off. This disease greatly reduced the landscape effect of plants. An oomycete species was isolated from the roots of the infected plants. This colony morphology was slightly radial to stellate, and the aerial mycelium was flocculent. Oval sporangia with papillae, apical chlamydospores and zoospores formed in sporangia were observed. The morphological characteristics were consistent with Phytophthora. For accurate identification, the internal transcribed spacer (ITS), cytochrome oxidase subunit II (COXII) and large ribosomal subunit (LSU) genes were amplified and sequenced. The species was identified as Phytophthora nicotianae using phylogenetic analysis. Finally, the disease was reproduced by inoculating healthy F. japonica with a zoospore suspension; the symptoms were consistent with those of natural infections, and the isolate obtained from artificially infected plants had the same morphological characteristics as the inoculated isolate. The results demonstrated that P. nicotianae is the pathogenic factor of root rot. of F. japonica. This is the first report of root rot on F. japonica caused by P. nicotianae in China.

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First Report of Phytophthora cinnamomi Causing Root and Stem Rot of Blueberry (Vaccinium corymbosum) in China

Cited by 8 publications

References 5 publications

Rapid detection of Phytophthora cinnamomi based on a new target gene Pcinn13739

Rapid detection of Phytophthora cinnamomi based on a new target gene Pcinn13739

A study of Phytophthora spp. in declining highbush blueberry in Germany reveals that P. cinnamomi can thrive under Central European outdoor conditions

The New Report of Root Rot on Fatsia japonica Caused by Phytophthora nicotianae in China

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