First Report of <i>Pectobacterium punjabense</i> Causing Blackleg and Soft Rot on Potato in Hebei and Fujian Province, China

Handique, Utpal; Cao, Yaning; Wang, Dekang; Zhang, Ruofang; Li, Wensi; Sun, Qinghua; Feng, Zhiwen; Wu, Jian

doi:10.1094/pdis-12-21-2731-pdn

Cited by 9 publications

(15 citation statements)

References 8 publications

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“…Potato stem rot caused by P. versatile (Han et al, 2022) and P. parvum (Wang et al, 2022a) was reported in the Fengning region in the summer of 2020; therefore, seven Pectobacterium species have been identified in potato plants in Hebei Province to date. MLSA demonstrated that a greater diversity of Pectobacterium infects potatoes at the province scale than previously reported: P. carotovorum, P. atrosepticum, P. brasiliense, P. parmentieri, P. polaris and P. punjabense in Guangdong, Inner Mongolia and Sichuan (Zhang et al, 2012;She et al, 2013;Zhao et al, 2018;Jiang et al, 2019;Cao et al, 2021;Handique et al, 2022a;Handique et al, 2022b). However, the P. carotovorum complex has been revised several times, and it is possible that some earlier isolated P. carotovorum strains might have been misclassified.…”

Section: Discussionmentioning

confidence: 91%

“…Blackleg and aerial stem rot of potatoes caused by SRP have become more frequent in China in recent years (Zhao et al, 2018;Cao et al, 2021;Han et al, 2022;Handique et al, 2022a;Wang et al, 2022a;Handique et al, 2022b). Potato stem rot caused by P. versatile (Han et al, 2022) and P. parvum (Wang et al, 2022a) was reported in the Fengning region in the summer of 2020; therefore, seven Pectobacterium species have been identified in potato plants in Hebei Province to date.…”

Section: Discussionmentioning

confidence: 99%

“…P. atrosepticum and P. carotovorum were once regarded as the most frequently isolated pathogens that are associated with potato soft rot diseases in China (Zhang et al, 2012;She et al, 2013). In recent years, other Pectobacterium species, including P. brasiliense (Zhao et al, 2018;Jiang et al, 2019), P. parmentieri (Cao et al, 2021), P. versatile (Han et al, 2022), P. polaris (Handique et al, 2022a) and P. punjabense (Handique et al, 2022b), have also been reported in the primary potato growing areas in China. Two citrate negative isolates of P. polaris were reported to cause aerial stem rot in Hebei Province, and subsequent genome-based analysis confirmed that they belonged to the newly established species, P. parvum (Wang et al, 2022a;Wang et al, 2022b).…”

Section: Introductionmentioning

confidence: 99%

“…The incidence of blackleg and aerial stem rot of potatoes has increased over recent years in northern China, and SRP has caused significant yield loss in highly susceptible cultivars, including Xisen 6, Huangxin 226, Helan 15 (also known as Favorita), and others (Zhao et al, 2018;Cao et al, 2021;Han et al, 2022;Handique et al, 2022a;Wang et al, 2022a;Handique et al, 2022b). In 2021, potato blackleg and aerial stem rot were reported in the three primary production areas in Hebei Province.…”

Section: Introductionmentioning

confidence: 99%

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Identification and characterization of opportunistic pathogen Pectobacterium polonicum causing potato blackleg in China

Han

Wang²,

Pirhonen³

et al. 2023

Front. Plant Sci.

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Blackleg and aerial stem rot of potato (Solanum tuberosum L.), caused by soft rot enterobacteria of the genera Pectobacterium and Dickeya, has recently increased years in Hebei Province, China. Field surveys were performed during the 2021 potato growing season in Hebei to identify and characterize bacterial pathogens. Sixteen potato plants showing blackleg or aerial stem rot were collected from three potato-producing areas, and ten representative pectinolytic bacteria were isolated from symptomatic plants. 16S rDNA sequencing and multilocus sequence analysis were performed to determine the taxonomic position of the bacterial isolates. The isolates belonged to the genus Pectobacterium, including Pectobacterium atrosepticum, Pectobacterium carotovorum, Pectobacterium brasiliense, and Pectobacterium parmentieri. The exceptions were isolates BY21311 and BY21312, which belonged to a new species of Pectobacterium polonicum previously found in groundwater. The taxonomy of isolate BY21311 was confirmed using whole genome-based analysis. P. polonicum has only been identified in potato plants on one farm in Baoding region in China. Isolates BY21311 and BY21312 displayed similar physiological and biochemical traits to the type strain DPMP315T. Artificial inoculation assays revealed that isolate BY21311 fulfilled Koch’s postulates for potato blackleg. These findings represent the first time P. polonicum, a water-associated Pectobacterium species may be the cause of blackleg in the field. Interestingly, P. polonicum BY21311 has reduced ability to macerate potato tubers when compared to P. atrosepticum, P. brasiliense, P. versatile, and P. parvum, which is more virulent in tubers than the type strain DPMP315T. The host range of isolate BY21311 was determined by injection method, which can impregnate five plants. Although the genome of isolate BY21311 harbors gene clusters encoding a type III secretion system, it did not elicit a hypersensitive response (HR) in Nicotiana benthamiana or N. tabacum leaves. T3SS effector AvrE and T4SS effector PilN were obtained by predicting isolate BY21311 genome. P. polonicum appears to show significant variations in gene content between two genomes, and gene content varies between isolates BY21311 and DPMP315T, with strain specific-genes involved in many aspects, including lipopolysaccharide biosynthesis, substrate translocation, T4SS and T6SS among others, suggesting that isolates BY21311 and DPMP315T might represent distinct clades within the species.

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Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Identification and characterization of opportunistic pathogen Pectobacterium polonicum causing potato blackleg in China

Han

Wang²,

Pirhonen³

et al. 2023

Front. Plant Sci.

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show abstract

“…P. atrosepticum and P. carotovorum were once regarded as the most frequently isolated pathogens that are associated with potato soft rot diseases in China (Zhang et al, 2012;She et al, 2013). In recent years, other Pectobacterium species, including P. brasiliense Jiang et al, 2019), P. parmentieri (Cao et al, 2021), P. versatile (Han et al, 2022), P. polaris (Handique et al, 2022a) and P. punjabense (Handique et al, 2022b), have also been reported in the primary potato growing areas in China. Two citrate negative isolates of P. polaris were reported to cause aerial stem rot in Hebei Province, and subsequent genome-based analysis confirmed that they belonged to the newly established species, P. parvum (Wang et al, 2022a;Wang et al, 2022b).…”

Section: Introductionmentioning

confidence: 99%

Detection, characterization, and management of plant pathogens

2024

Frontiers Research Topics

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Frontiers is more than just an open access publisher of scholarly articles: it is a pioneering approach to the world of academia, radically improving the way scholarly research is managed. The grand vision of Frontiers is a world where all people have an equal opportunity to seek, share and generate knowledge. Frontiers provides immediate and permanent online open access to all its publications, but this alone is not enough to realize our grand goals. Frontiers journal seriesThe Frontiers journal series is a multi-tier and interdisciplinary set of openaccess, online journals, promising a paradigm shift from the current review, selection and dissemination processes in academic publishing. All Frontiers journals are driven by researchers for researchers; therefore, they constitute a service to the scholarly community. At the same time, the Frontiers journal series operates on a revolutionary invention, the tiered publishing system, initially addressing specific communities of scholars, and gradually climbing up to broader public understanding, thus serving the interests of the lay society, too. Dedication to qualityEach Frontiers article is a landmark of the highest quality, thanks to genuinely collaborative interactions between authors and review editors, who include some of the world's best academicians. Research must be certified by peers before entering a stream of knowledge that may eventually reach the public -and shape society; therefore, Frontiers only applies the most rigorous and unbiased reviews. Frontiers revolutionizes research publishing by freely delivering the most outstanding research, evaluated with no bias from both the academic and social point of view. By applying the most advanced information technologies, Frontiers is catapulting scholarly publishing into a new generation. What are Frontiers Research Topics?Frontiers Research Topics are very popular trademarks of the Frontiers journals series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area.

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Isolation and characterization of lytic bacteriophages infecting Pectobacterium atrosepticum

Wu,

Liu,

Liu

et al. 2024

Eur J Plant Pathol

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Pectobacterium spp. cause blackleg and soft rot on potato worldwide. Potato is a staple crop in China, and blackleg and soft rot diseases caused by Pectobacterium spp. have increased signi cantly as potato cultivation expanded rapidly in the last decade. This study was conducted to isolate and characterize the lytic bacteriophages infecting the major pathogen causing potato blackleg and soft rot, Pectobacterium atrosepticum (P. a.), in water and soil samples collected in the cities Wuhan and Datong in China. Three lytic bacteriophages were obtained and named Pectobacterium Phages ZRIMU1, 2, and 7. Transmission electron microscopic analysis revealed that all three phages were similar in size, with a smooth head (diameter ca. 40-50 nm) and without a neck or tail. The adsorption of phages PP1, PP2, and PP7 to P. a. after 18, 27, and 18 min was 98, 99 and 98%, respectively. The latent periods for PP1, PP2, and PP7 were 20, 40, and 50 min, respectively. In vitro bacterial challenge assays showed that these three phages were able to infect P. a. 1 h after coinoculation. The potential of these bacteriophages to protect potato seedlings was tested. After 27 days of inoculation, seedlings inoculated with water and sprayed with or without phages developed well, and no symptoms developed. Seedlings inoculated with P. a. only had a signi cantly lower survival rate than the controls, and seedlings coinoculated with P. a. and phages signi cantly improved the survival rate by 40%. Recovery of symptomatic plants was observed in groups that were treated with either a single phage or a mixture of phages. More investigations are needed to clarify the mechanism by which phages can be used to cure symptomatic potato plants.

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First Report of Pectobacterium punjabense Causing Blackleg and Soft Rot on Potato in Hebei and Fujian Province, China

Cited by 9 publications

References 8 publications

Identification and characterization of opportunistic pathogen Pectobacterium polonicum causing potato blackleg in China

Identification and characterization of opportunistic pathogen Pectobacterium polonicum causing potato blackleg in China

Detection, characterization, and management of plant pathogens

Isolation and characterization of lytic bacteriophages infecting Pectobacterium atrosepticum

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