First Report of <i>Pectobacterium carotovorum</i> subsp. <i>brasiliense</i> Causing Blackleg and Stem Rot Disease of Potato in Russia

Воронина, М. В.; Kabanova, Anastasia P.; Shneider, Mikhail M.; Korzhenkov, Aleksei A.; Toschakov, Stepan V; Miroshnikov, Kirill K.; Miroshnikov, Konstantin A.; Игнатов, А. Н.

doi:10.1094/pdis-03-18-0456-pdn

Cited by 23 publications

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“…On the basis of available studies reporting the presence of Pbr in the host plants, more than 50% of them have been associated with potato (this study, Table 1 ). In fact, Pbr has been reported in potato in Brazil since 2004 [ 11 ], followed by its detection in North America [ 66 , 67 , 68 ], Europe [ 12 , 13 , 14 , 69 , 70 ], Africa [ 7 , 9 , 71 , 72 ], Asia [ 62 , 73 , 74 , 75 , 76 ], and New Zealand [ 77 ]. Generally, Pbr causes blackleg and soft rot in potato leaves, stems, and tubers [ 8 , 10 , 66 , 68 , 74 ].…”

Section: Symptoms Host Range and Geographical Distributionmentioning

confidence: 99%

“…Pbr has been reported in Eurasia: from Western European countries to Russia and China [ 4 , 5 , 6 ], in Africa: South Africa, Morocco, Algeria and Kenya [ 7 , 8 , 9 , 10 ]. Pbr has been constantly reported since 2004 causing significant losses particularly of potato ( Solanum tuberosum L.) [ 11 , 12 , 13 , 14 ] and posing a threat to worldwide potato gross production value which is estimated to be $63914 million in 2018 [ 15 ]. Significant disease caused by Pbr has been pointed out in Belgium, the Netherlands and Switzerland: for instance, in 2015 in Switzerland prevalence of Pbr reached 70% of the samples collected from potato field outbreaks [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

“…Significant disease caused by Pbr has been pointed out in Belgium, the Netherlands and Switzerland: for instance, in 2015 in Switzerland prevalence of Pbr reached 70% of the samples collected from potato field outbreaks [ 13 ]. In Russia, in 2017 more than 20% soft rot disease was caused by Pbr and this species also has been reported as a predominant cause of blackleg of potato in the Moscow region in 2018 [ 14 ]. However, in some other countries such as the United Kingdom and Norway, so far Pbr is not considered as a problematic species in potato fields [ 16 , 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

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Pectobacterium brasiliense: Genomics, Host Range and Disease Management

et al. 2021

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Pectobacterium brasiliense (Pbr) is considered as one of the most virulent species among the Pectobacteriaceae. This species has a broad host range within horticulture crops and is well distributed elsewhere. It has been found to be pathogenic not only in the field causing blackleg and soft rot of potato, but it is also transmitted via storage causing soft rot of other vegetables. Genomic analysis and other cost-effective molecular detection methods such as a quantitative polymerase chain reaction (qPCR) are essential to investigate the ecology and pathogenesis of the Pbr. The lack of fast, field deployable point-of-care testing (POCT) methods, specific control strategies and current limited genomic knowledge make management of this species difficult. Thus far, no comprehensive review exists about Pbr, however there is an intense need to research the biology, detection, pathogenicity and management of Pbr, not only because of its fast distribution across Europe and other countries but also due to its increased survival to various climatic conditions. This review outlines the information available in peer-reviewed literature regarding host range, detection methods, genomics, geographical distribution, nomenclature and taxonomical evolution along with some of the possible management and control strategies. In summary, the conclusions and a further directions highlight the management of this species.

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Section: Symptoms Host Range and Geographical Distributionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Pectobacterium brasiliense: Genomics, Host Range and Disease Management

et al. 2021

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“…Of these, Pectobacterium carotovorum subsp. brasiliense is an emerging SRE with global distribution (van der Merwe et al, 2010; Panda et al, 2012; Onkendi and Moleleki, 2014; Moraes et al, 2017; Ma et al, 2018; Voronina et al, 2019). Previous studies have shown that Pcb strain PBR1692 (referred to henceforth as Pcb 1692) is able to inhibit growth of Pectobacterium carotovorum subsp.…”

Section: Introductionmentioning

confidence: 99%

The Impact of Type VI Secretion System, Bacteriocins and Antibiotics on Bacterial Competition of Pectobacterium carotovorum subsp. brasiliense and the Regulation of Carbapenem Biosynthesis by Iron and the Ferric-Uptake Regulator

et al. 2019

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The complexity of plant microbial communities provides a rich model for investigating biochemical and regulatory strategies involved in interbacterial competition. Within these niches, the soft rot Enterobacteriaceae (SRE) represents an emerging group of plant–pathogens causing soft rot/blackleg diseases resulting in economic losses worldwide in a variety of crops. A preliminary screening using next-generation sequencing of 16S rRNA comparatively analyzing healthy and diseased potato tubers, identified several taxa from Proteobacteria to Firmicutes as potential potato endophytes/plant pathogens. Subsequent to this, a range of molecular and computational techniques were used to determine the contribution of antimicrobial factors such as bacteriocins, carbapenem and type VI secretion system (T6SS), found in an aggressive SRE (Pectobacterium carotovorum subsp. brasiliense strain PBR1692 – Pcb1692) against these endophytes/plant pathogens. The results showed growth inhibition of several Proteobacteria by Pcb1692 depends either on carbapenem or pyocin production. Whereas for targeted Firmicutes, only the Pcb1692 pyocin seems to play a role in growth inhibition. Furthermore, production of carbapenem by Pcb1692 was observably dependent on the presence of environmental iron and oxygen. Additionally, upon deletion of fur, slyA and expI regulators, carbapenem production ceased, implying a complex regulatory mechanism involving these three genes. Finally, the results demonstrated that although T6SS confers no relevant advantage during in vitro competition, a significant attenuation in competition by the mutant strain lacking a functional T6SS was observed in planta.IMPORTANCESoft rot Enterobacteriaceae (SRE) represents important phytopathogens causing soft rot/blackleg diseases in a variety of crops leading to huge economic losses worldwide. These pathogens have been isolated alongside other bacteria from different environments such as potato tubers, stems, roots and from the soil. In these environments, SREs coexist with other bacteria where they have to compete for scarce nutrients and other resources. In this report, we show that Pectobacterium carotovorum subsp. brasiliense strain PBR1692 – Pcb1692, which represents one of the SREs, inhibits growth of several different bacteria by producing different antimicrobial compounds. These antimicrobial compounds can be secreted inside or outside the plant host, allowing Pcb1692 to effectively colonize different types of ecological niches. By analyzing the genome sequences of several SREs, we show that other SREs likely deploy similar antimicrobials to target other bacteria.

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“…Pectobacterium spp. are necrotrophic bacterial pathogens that cause a wide range of diseases such as soft rot, stem wilt, and blackleg on a variety of plant hosts all over the world (Charkowski 2018;Kim and Jeon 2016;Lu et al 2020;Voronina et al 2019;Zlatković et al 2019). Many species belong to the family Pectobacteriaceae.…”

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confidence: 99%

Complete Genome Sequence Resource for the Necrotrophic Plant-Pathogenic Bacterium Pectobacterium carotovorum WPP14

et al. 2021

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Pectobacterium spp. are a major cause of loss in vegetable and ornamental plant production. One of these species, Pectobacterium carotovorum, can cause soft rot disease on many plants, particularly potatoes. These diseases lead to significant economic loss and pose food security threats by reducing crop yields in the field, in transit, and during storage. The Gram-negative enterobacterium, P. carotovorum WPP14, is a particularly virulent strain for which there is no available closed genome, limiting the molecular research for this important pathogen. Here, we report a high-quality complete and annotated genome sequence of P. carotovorum WPP14. The 4,892,225 bp genome was assembled with Nanopore reads and polished with Illumina reads, yielding 394× and 164× coverage, respectively. This closed genome provides a resource for research on improved detection and biology of P. carotovorum, which could translate into improved disease management.

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First Report of Pectobacterium carotovorum subsp. brasiliense Causing Blackleg and Stem Rot Disease of Potato in Russia

Cited by 23 publications

References 3 publications

Pectobacterium brasiliense: Genomics, Host Range and Disease Management

Pectobacterium brasiliense: Genomics, Host Range and Disease Management

The Impact of Type VI Secretion System, Bacteriocins and Antibiotics on Bacterial Competition of Pectobacterium carotovorum subsp. brasiliense and the Regulation of Carbapenem Biosynthesis by Iron and the Ferric-Uptake Regulator

Complete Genome Sequence Resource for the Necrotrophic Plant-Pathogenic Bacterium Pectobacterium carotovorum WPP14

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